Substrates
Bioworld BCIP/INT, 100 mL
BCIP/INT A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. Used for in situ hybridizations, when tissues will be counterstained with hematoxylin. The brick-red end product is clearly visible against a blue hematoxylin background. Technical Information:Ready to use straight from the bottle.MF: C8H6BrCINO4P C7H9NCAS: 6578-06-9MW: 433.62Ultra Pure GradePurity (enzymatic): >99.0%Purity (UV): >99.0%Inhibitors (enzymatic): <0.1%Solubility (0.1% phosphate buffer): Clear and haze-freeBlank (A260, 1mg/ml, DMF): 0.010Solutions are stable for 6 months after opening when stored cold.Storage: -15 to -22 CRef: Unger, et al., Am. J. Surg. Pathol., 10(1): 1-8, 1986.
Sino Biological Peptide Substrates: S6K Substrate
The S6K sub peptide sequence (KRRRLASLR) is based on human 40S ribosomal protein S6 (amino acid 230-238).
Bachem Suc-Ala-Phe-Pro-Phe-pNA
Suc-Ala-Phe-Pro-Phe-pNA, 128802-73-3, C36H40N6O9, Mr 700.75, 50mg. Substrate for FK-506 binding proteins (FKBPs, also called macrophilins) and cyclophilins, which belong to the group of peptidyl prolyl cis-trans isomerases (PPIases). Suc-AFPF-pNA has been used for an uncoupled protease-free assay of PPIase activity.
Medchemexpress LLC Ac-YEVD-AMC | 1631716-11-4 | 99.6% | 723.73 | 10 MG
Ac-YEVD-AMC is a fluorescent substrate of caspase-1/11. It releases amino-4-methylcoumarin (AMC) for fluorescence detection when treated with cell lysate.
- Fluorescent dye
- Releases amino-4-methylcoumarin (AMC) for fluorescence detection
Abcam Magenta-Gal, Chromogenic beta-galactosidase substrate, 100MG
MW 408.63 g/mol. Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product. Used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.
The product is subject to the following: Abcam Restricted Use Statement
Echelon Biosciences Research Labs Autotaxin Inhibitor Screening Kit
The Autotaxin Inhibitor Screening Kit utilizes purified autotaxin (ATX) and the autotaxin substrate FS-3, which fluoresces upon cleavage. The assay is performed in a 96 well plate and quantifies the autotaxin activity in the presence of the users samples by measuring changes in fluorescence over time.Sample Type: Inhibitors against ATXSample Volume: 10 L/ well of your sample compoundsSample Considerations: Some organic solvents interfere with the assay (see technical data sheet). This item is non returnable
Apexbio Technology LLC Luminescent ATP Detection Assay Kit(Synonyms: ATP Luminescence Assay Kit, ATP Bioluminescent Assay Kit, ATP Determination Kit), 200T.
Luminescent ATP Detection Assay Kit employs firefly luciferase-mediated bioluminescence for quantitative analysis of ATP concentration Luciferase catalyzes oxidation of the substrate D-luciferin in the presence of ATP resulting in emission of luminescence with intensity directly proportional to ATP levels This assay enables sensitive measurement within a linear detection range between 1 nM and 10 M ATP applicable for solution cell and tissue samples without complex extraction The detection procedure maintains stable luminescent signals for up to 30 min and samples remain compatible for further downstream analyses such as protein quantification SDS-PAGE and Western blotting
Medchemexpress LLC Anti-Human Rat HER2 5mg | 5mg
Anti-Human/Rat HER2 (neu) Antibody (7 16 4) is an anti-mouse HER2 IgG2a monoclonal antibody Anti-Human/Rat HER2 (neu) Antibody (7 16 4) reduces tumor cell proliferation by inhibiting the HER2 signaling pathway Anti-Human/Rat HER2 (neu) Antibody (7 16 4) can activate CD8 T cells Anti-Human/Rat HER2 (neu) Antibody (7 16 4) can be used for research on cancer such as breast cancer[1][2]
Medchemexpress LLC D-luciferin sodium | 103404-75-7 | MFCD00044938 | >95.0% | 302.3 g/mol | C11H7N2NaO3S2 | 100MG
D-luciferin sodium is the water-soluble sodium salt of firefly luciferin used as a chemiluminescent substrate for luciferase assays in vitro and in vivo. Supplied as a powder, it dissolves readily in aqueous or organic solvents to prepare assay solutions for reporter gene assays, bioluminescence imaging, and ATP-dependent measurements.
- Water-soluble sodium salt suitable for luciferase-based bioluminescence assays.
- High purity (>95%) for reliable assay performance.
- Soluble in water and DMSO (solubility reported to ~25 mM) for easy preparation.
- Provided as a lyophilized powder for accurate dosing and storage.
- Stable when stored desiccated at -20°C and protected from light.
Medchemexpress LLC Z-Gly-Pro-AMC | 68542-93-8 | 99.5% | 463.48 | 1 MG
Z-Gly-Pro-AMC is a fluorogenic substrate that is hydrolyzed by prolyl endopeptidase to produce highly fluorescent 7-Amino-4-methylcoumarin.
- Hydrolyzed by prolyl endopeptidase.
- Generates highly fluorescent 7-Amino-4-methylcoumarin.
Medchemexpress LLC HY-15935 50mg Medchemexpress, X-Gluc (Dicyclohexylamine) CAS:18656-96-7 Purity:>98%
Medchemexpress, HY-15935 50mg X-Gluc (Dicyclohexylamine) CAS:18656-96-7 Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Medchemexpress LLC Carboxybenzoyl-leucyl-arginine 7-amido-4-methylcoumarin | 156192-32-4 | 99.2% | 578.66 g/mol | C30H38N6O6 | 5 MG
Z-Leu-Arg-AMC is a fluorogenic peptide substrate for assaying cysteine proteases such as cathepsin K. It consists of a carboxybenzoyl (Z)-protected leucyl-arginine linked to 7-amido-4-methylcoumarin (AMC); enzymatic cleavage releases fluorescent AMC for sensitive kinetic measurements. Supplied as a solid or as a 10 mM solution in DMSO for research use only.
- Sensitive fluorogenic reporter released on protease-mediated cleavage.
- Suitable for cathepsin K and other cysteine protease activity assays.
- High reported purity for consistent, reproducible results.
- Available as solid or pre-prepared DMSO solution for flexible use.
- Soluble in DMSO; handle and store according to standard reagent recommendations.
Medchemexpress LLC Glp-Asn-Pro-AMC | 291752-43-7 | 99.3% | 497.50 | 25 MG
Glp-Asn-Pro-AMC is identified as an inhibitor of thyrotropin-releasing hormone degrading enzyme (TRH-DE), exhibiting a Ki value of 0.97 μM.
- Inhibitor of thyrotropin-releasing hormone degrading enzyme (TRH-DE)
Selleck Chemical LLC D-Luciferin
D-Luciferin is a popular bioluminescent substrate of luciferase in the presence of ATP used in luciferase-based bioluminescence imaging and cell-based high-throughput screening applications In an immunocompetent mouse model of ovarian cancer the use of D-luciferin substrate and firefly luciferase preserves tumour-host immune interactions since bioluminescent imaging is a more sensitive indication of tumour growth than weight gain
