Substrates
Gold Biotechnology Inc D-Luciferin, Potassium Salt (Proven and Published™) 300 mg
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
AdipoGen L-Leucine-7-amido-4-methylcoumarin hydrochloride
L-Leucine-7-amido-4-methylcoumarin hydrochloride, AdipoGen Life Sciences. Chemical. CAS: 62480-44-8. Formula: C16H20N2O3 . HCl. MW: 324.8. Synthetic. Fluorogenic substrate for leucine aminopeptidase. Sensitivity of fluorescence detection is greatly increased over other methods such as 4-Methoxy-naphthylamide (pNA) assays. 62480-44-8
Sino Biological Peptide Substrates: IKKtide
The IKKtide peptide sequence (KKKKERLLDDRHDSG-LDSMKDEE) is derived from human IkBA (amino acids 21-41) and is suitable as a substrate for IKK alpha and IKK beta.
Gold Biotechnology Inc D Luciferin Potassium Salt 10g
L uciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2 as cofactors to emit a characteristic yellow-green emission in the presence of oxygen which shifts to red light in vivo at 37 C Through the utilization of ATP the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain
Research Products International Corp IPTG, 200 G
IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.
Dioxane-Free
Water Content: <0.49%
Revvity Health Sciences Inc Tag-lite CLIP-Lumi-Tb Labeling Reagent, 20 nmoles
Tag-lite CLIP-Lumi-Tb Labeling Reagent, 20 nmoles
Ubpbio SUC-LLVY-AMC, 5MG
Fluorescent Peptides/Protein Substrates – SUC-LLVY-AMC (UBPBio catalog# G1100, G1101) – Suc-LLVY-AMC is a fluorogenic substrate for the chymotrypsin – like activity of the 20S and 26S proteasomes. Working concentrations of this substrate is 50-200 µM. The released AMC can be detected by a fluorimeter or plate reader at excitation/emission wavelengths of 380 nm/460 nm, respectively. When used to determine proteasome activity in cell lysates, cell lysates that are pre-treated with a proteasome inhibitor such as MG132, PS341 or epoxomicin can be used to determine the fluorescence contributed by other cellular proteases that cleave this substrate. Readings from proteasome inhibitor-treated lysates should be subtracted.
Enzo Life Sciences Ac-IEPD-pNA (5mg)
Colorimetric granzyme B substrate. Combinatorial and kinetic studies have found the IEPD sequence to be optimal for cleavage of a tetrapeptide substrate by recombinant granzyme B, with a Km for Ac-IEPD-pNA of 57 µM. Cleavage of the substrate by granzyme B releases pNA, which can be detected by its absorbance at 405 nm. Alternative name: Caspase 8 substrate (chromogenic), Granzyme B substrate (chromogenic). Purity: ≥95%. Solubility: Soluble to at least 100mM in DMSO. Long Term Storage: -20°C.
Enzo Life Sciences TACE substrate II (fluorogenic) (100µg)
Sensitive fluorogenic substrate for TACE (TNFα converting enzyme, ADAM17). Also cleaved by ADAM10 and likely other ADAMs and MMPs. The kcat/Km of TACE for this substrate is 7-fold higher than for the same peptide with the Mca-Dpa fluorophore-quencher pair. FAM fluorescence is quenched by the TAMRA group until cleavage separates them. Ex.: 485nm, Em.: 535nm, although the following Ex/Em can also be used: 490,494/515,520nm. This substrate is useful for inhibitor screening, kinetic analysis, and cellular activity assay. Alternative name: ADAM17 substrate 2 (fluorogenic). Purity: ≥95% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Enzo Life Sciences OMNICATHEPSIN fluorogenic substrate (50mg). CAS: 65147-22-0
Fluorogenic substrate cleaved by plasma kallikrein and kallikrein 8, but not plasmin; by papain; and by most cathepsins (cathepsins B, C/DPP-I, F, K/O2, L, L2/V, O, S, and X/Z). Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144). Alternative name: Z-FR-AMC, Z-Phe-Arg-AMC. Purity: ≥99%. Long Term Storage: -20°C.
Medchemexpress LLC HY-15921 5g , IPTG Isopropyl β-D-thiogalactoside CAS:367-93-1 Purity:98%
Medchemexpress, HY-15921 5g IPTG Isopropyl β-D-thiogalactoside CAS:367-93-1 Formula:C9H18O5S Purity:98% IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Sigma Aldrich Fine Chemicals Biosciences SIGMAFAST(TM) OPD tablet | 50SET
SIGMAFAST(TM) OPD tablet | 50SET
Abcam AEC Substrate Kit, 125ML
AEC substrate kit for use in IHC using HRP / peroxidase detection.
The product is subject to the following: Abcam Restricted Use Statement
AG Scientific Inc IPTG, 10 G
IPTG known as Isopropyl-Beta-D-Thiogalactopyranoside functions as an inducer of galactosidase activity by binding to and inhibiting the repressor.It is utilized for the induction of expression from the lac promoter and derivates. IPTG is also used to differentiate recombinants from non-recombinants in cloning strategies using vectors containing the Z or Z peptide gene.CAS Number: 367-93-1Molecular Weight: 238.3 DaChemical Formula: C9H18O5SSolubility: Soluble in water (5%), renders clear colorless solutionStorage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.