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The system is a higher-throughput platform that leverages BD proprietary, gentle, robust microwell-based single-cell partitioning technology to perform single cell analysis. The system is capable of single-cell capture and barcoding enabling capture of more than 640,000 cells per cartridge.
The BD Rhapsody™ ATAC-Seq Assays enable you to either generate highly reproducible open chromatin profiling data from single cells or perform a multiomic analysis of open chromatin accessibility and transcriptome of single cells in one experiment using the BD Rhapsody™ Single-Cell Analysis System.
The BD Rhapsody™ Scanner is designed to visualize all steps in the single-cell capture workflow and provide detailed analysis metrics at every step, enabling the user to make key decisions throughout the workflow.
The next generation of BD Rhapsody™ TCR/BCR Multiomic Assays reveal immune repertoire richness with great sensitivity alongside simultaneous single-cell gene and protein expression analyses.
The BD Rhapsody™ Intracellular AbSeq Buffer Kit allows users to stain for intracellular antigens using intracellular BD™ AbSeq Antibody-Oligos for profiling with the BD Rhapsody™ System.
BD AbSeq Immune Discovery Panel is developed using BD antibody-oligo based technology and consists of 30 different specificities against major human immune markers lyophilized in a single tube.
The SeqPlex-I WTA kit allows amplification of small quantities of reverse transcribed RNA or degraded RNA for direct input onto Illumina(R) next-generation sequencing (NGS) flow cells. The SeqPlex-i process is comprised of three steps Pre-amplification/Library Synthesis Amplification 1 and Amplification 2Step 1 In the Pre-amplification/Library Synthesis step using the (Library Preparation Reagents) the template RNA is reverse transcribed using primers composed of a semi-degenerate 3- and universal 5-ends. As polymerization proceeds displaced and RNaseH generated single strands serve as new templates for additional primer annealing and extension producing random overlapping cDNAs flanked by a universal primer (5) and primer complement (3) sequence.Step 2 In the Amplified Library Synthesis step (using the Amplification 1 Reagents) products from pre-amplification/library synthesis are amplified by single primer PCR via the universal end sequence. These amplification products typicall
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