Bioassay Systems

Bioassay Systems Phosphatase Inhibitor. Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems vanadate reagent is activated for maximum inhibition. Uses: to preserve protein phosphorylation state in cells and lysates.

Sodium Orthovanadate is a general inhibitor for protein phosphotyrosyl phosphatases. BioAssay Systems vanadate reagent is activated for maximum inhibition. Example of uses: preserve protein phosphorylation state in cells and lysates, and use as a standard phosphatase inhibitor in phosphatase assays. Key Features: Ready-to-use phosphatase inhibitor. Fully activated for maximum inhibition. Kit size: 1 mL 100 mM. Sodium Orthovanadate (CAS Number 13721-39-6, Na3VO4) is a general inhibitor for protein phosphotyrosyl phosphatases (PTPs). Inhibition of vanadate is competitive and can be reversed by addition of EDTA or by dilution. Vanadate is commonly used to preserve the protein tyrosyl phosphorylation state in cells and lysates. BioAssay Systems Vanadate reagent is activated for maximum inhibition. Suggested Working Concentration: 1-10 mM

Bioassay Systems QuantiQuik™ Urea (BUN) Quick Test Strips. Direct determination of urea concentrations in food and beverage samples as well as biological samples.

For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

Bioassay Systems QuantiChrom™ Glucose Dehydrogenase Assay Kit. For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism.

For quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 200 U/L for 15 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, tissue and culture media). Species: All. Procedure: Assay takes 15 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

Bioassay Systems EnzyLight™ Cytotoxicity Assay Kit. For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxicity and high-throughput screen for anticancer agents.

For rapid, bioluminescent luciferin/luciferase based assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 50 cells can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.6 to 0.7 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence. Samples: Cells. Species: All. Procedure: Assay takes 2 min. Kit size: 100 tests. Detection limit: 50 cells.

Bioassay Systems EnzyChrom™ Nitric Oxide Synthase Inhibitor Screening Kit. HTS for inhibitor screening and evaluation of nitric oxide synthase inhibitors.

For evaluation and high-throughput screen (HTS) of nitric oxide synthase (NOS) modulators. Key Features: High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system. Rapid and reliable. Can be completed in less than 3 hours if assay performed at 37°C. Method: OD540nm. Samples: Nitric Oxide Synthase. Species: All. Procedure: Assay takes 2-3 hours. Kit size: 100 tests.

Bioassay Systems LEUCINE AMINOPEPTIDASE ASSAY

NC3397363 LEUCINE AMINOPEPTIDASE ASSAY

Bioassay Systems EnzyChrom™ Aspartate Assay Kit. For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.

For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism. Key Features: Sensitive and accurate. Linear detection range: 2 to 400 µM aspartate for colorimetric assays and 1 to 50 µM for fluorimetric assays. Method: OD570nm, or FL530/585nm. Samples: Plasma, serum, tissue, culture media etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 2, 1 µM.

Bioassay Systems EnzyChrom™ Invertase Assay Kit. For quantitative determination of invertase/sucrase activity.

For quantitative determination of invertase/sucrase enzyme activity. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological, environment (soil) etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.007 U/L.

Bioassay Systems QuantiFluo™ Chymotrypsin Inhibitor Assay Kit, For evaluation of drugs and screening of potential inhibitors to chymotrypsin proteases.

CHYMOTRYPSIN (EC 3.4.21.1) is a digestive, serine protease that hydrolyzes dietary proteins in many eukaryotic and prokaryotic organisms. Chymotrypsin predominantly cleaves peptide chains at the carboxyl side of the aromatic phenylalanine, tryptophan, and tyrosine amino acids. BioAssay System’s QuantiFluo™ Chymotrypsin Inhibitor Assay Kit uses a fluorescein isothiocyanate (FITC)-labeled synthetic substrate. The fluorescein label is highly quenched. Upon digestion by chymotrypsin present in the sample, the substrate is cleaved into smaller peptides, which abolishes the quenching of the fluorescence label. The fluorescence or fluorescence polarization (FP) of the FITC-labeled fragments is measured at λex/em = 485/530 nm. Inhibition is determined by the decrease in fluorescence.

Bioassay Systems QuantiFluo™ Pepsin Inhibitor Assay Kit, For evaluation of drugs and screening of potential inhibitors to pepsin proteases.

PEPSIN (EC 3.4.23.1) is a digestive, serine protease that hydrolyzes dietary proteins in many eukaryotic and prokaryotic organisms. Pepsin predominantly cleaves peptide chains at the amino side of the aromatic phenylalanine, tryptophan, and tyrosine amino acids. BioAssay Systems’ QuantiFluo™ Pepsin Inhibitor Assay Kit uses a fluorescein isothiocyanate (FITC)-labeled synthetic substrate. The fluorescein label is highly quenched. Upon digestion by pepsin present in the sample, the substrate is cleaved into smaller peptides, which abolishes the quenching of the fluorescence label. The fluorescence or fluorescence polarization (FP) of the FITC-labeled fragments is measured at λex/em = 485/530 nm. Inhibition is determined by the decrease in fluorescence.

Bioassay Systems QuantiChrom™ Peroxide Assay Kit II, Direct Assay of H2O2 and organic peroxides in biological samples (e.g. serum, citrate-plasma, urine, cell lysate, culture medium) and the determination of the effects of drugs on peroxide metabolism.

Peroxide is one of the key reactive oxygen species formed under oxidative stress conditions. High levels of peroxide formation have been linked to pathological conditions such as aging, asthma, diabetes, atherosclerosis, cataracts, inflammatory arthritis and neurodegenerative diseases. Simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. BioAssay Systems’ improved peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe3+-xylenol orange reaction, in which a purple complex is formed when Fe2+ provided in the reagent is oxidized to Fe3+ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Bioassay Systems EnzyChrom™ Aconitase Assay Kit. For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.

For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handlingsystems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

Bioassay Systems EnzyFluo™ Uricase Assay Kit, Uricase activity in biological samples such as cell culture, non-human biofluids, cell extracts, and plant tissue extracts.

URICASE or URATE OXIDASE (E.C. 1.7.3.3) initiates the oxidation of uric acid, eventually yielding allantoin. Uricase activity is found throughout plants, animals, and bacteria, but it is not found in hominids. As a result, humans develop gout when the levels of uric acid become too high. BioAssay Systems’ method provides a simple and high-throughput assay for measuring uricase enzyme activity. In this assay, uric acid is enzymatically converted to allantoin, releasing H2O2. The resulting H2O2 reacts with a specific dye to form a pink-colored product. The change in fluorescence intensity at 530/585 nm is directly proportional to the uricase activity in the sample.

Bioassay Systems QuantiChrom™ Pectinase Assay Kit. For quantitative determination of pectinase activity determination in biological samples.

For quantitative determination of pectinase activity determination in biological samples. Key Features: Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay. Simple and convenient 40 minute "Add-Mix-Measure" type assay. High-throughput format and compatible with laboratory liquid handling systems. No heating required. The kit does not use toxic materials. Refer to the previously established DNS method. Method: OD600nm. Samples: Enzyme extracts, agriculture and biological samples. Species: Plant, fungal tissues, juice, bacteria, etc. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 9.2 U/L.

Bioassay Systems EnzyFluo™ AMPK Phosphorylation Assay Kit. For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells.

For quantitative fluorescent immunoenzymatic assay of AMPK phosphorylation status in cultured cells. Key Features: Sensitive. Can measure pAMPK modulation in as little as 500 cells/well. New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL360/450nm, 530/585nm. Samples: Cells. Species: Human, mouse, rat. Procedure: Assay takes Assay takes 6.5 hrs, hands-on time 2.5 hrs. Kit size: 100 tests.