Bioassay Systems

Bioassay Systems QuantiQuik™ Urea (BUN) Quick Test Strips. Direct determination of urea concentrations in food and beverage samples as well as biological samples.

For rapid, direct determination of urea concentrations in food and beverage samples as well as biological samples. Key Features: Fast and sensitive. Use of 20 µL sample. Semi-quantitative measurement between 0-1500 mg/L (undiluted) urea. Convenient. No expensive plate or cuvette readers needed. Sample trea™ent and assay can be performed in under 15 minutes. Method: Visual. Samples: Serum, plasma, urine, etc. Species: All. Procedure: Assay takes approximately 15 min. Kit size: 10 tests.

Bioassay Systems QuantiChrom™ Protein Assay Kit. For quantitative determination of total protein.

For quantitative determination of total protein. Key Features: Sensitive and accurate. Use 10 mL samples. Detection range 0.06 - 1.0 mg /mL protein in 96-well plate assay. Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Low interference. Glucose, Tris, vitamins, and amino acids, DNA, RNA, salts, EDTA (< 12 mM), phenol (< 50 mM), urea (< 0.6 M), Triton (<0.1%) and SDS (< 0.1% SDS) do not interfere in the assay. Versatility: assays can be executed in 96-well plate or cuvet. Method: OD595nm (Bradford). Samples: Biological, food, agriculture etc. Species: All. Procedure: Assay takes 2 min. Kit size: 500 tests. Detection limit: 60 µg/mL.

Bioassay Systems EnzyChrom™ AF HDL and LDL/VLDL Assay Kit. For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism.

For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism. Key Features: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. Method: OD570nm, or FL530/585nm. Samples: Serum. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: OD, FL: 1, 0.2 mg/dL.

Bioassay Systems EnzyChrom™ Histamine Assay Kit. For quantitative determination of histamine in food, beverage and agricultural products.

For quantitative determination of histamine in food, beverage and agricultural products. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.0025 - 1 mM (0.3 - 111 ppm) histamine in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Food, beverage and agricultural products. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2.5 µM.

Bioassay Systems QuantiChrom™ Copper Assay Kit. For quantitative determination of copper(II) ion and evaluation of drug effects on copper metabolism.

For quantitative determination of copper(II) ion and evaluation of drug effects on Cu metabolism. Key Features: Sensitive and accurate. Linear detection range 7 µg/dL (1.0 µM) to 300 µg/dL (47 µM) copper in 96-well plate assay. Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Method: OD359nm. Samples: Biological, environment, food and beverage. Species: All. Procedure: Assay takes 10 min. Kit size: 250 tests. Detection limit: 7 µg/dL (1.0 µM).

Bioassay Systems EnzyChrom™ L-Amino Acid Assay Kit. Quantitative determination of L-Amino Acids in biological and other samples.

For quantitative determination of L-Amino Acids in biological and other samples. Key Features: Fast and sensitive. Linear detection range: 3.3 to 500 µM (colorimetric assay) and 0.13 to 50 µM (fluorimetric assay) for 60 min reaction. Convenient. The procedure involves adding a single working reagent and reading after 60 minutes. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated to process thousands of samples per day. Method: OD570nm or FL530/585nm. Samples: Serum, culture media, tissue homogenates, cell lysates, urine, food samples, etc. Species: All. Procedure: Assay takes 60 min. Kit size: 100 tests. Detection limit: 3.3 µM (colorimetric assay), 0.13 µM (fluorimetric assay).

Bioassay Systems EnzyChrom™ Malate Dehydrogenase Assay Kit. For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism.

For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 65 U/L for 20 min reaction at 37°C. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD565nm. Samples: Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 U/L.

Bioassay Systems EnzyChrom™ Monoamine Oxidase Assay Kit. For rapid, quantitative determination of monoamine oxidase activity and MAO inhibitor screen.

For rapid, quantitative determination of monoamine oxidase activity and MAO inhibitor screen. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L MAO activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: MAO enzyme preparations. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.01 U/L.

Bioassay Systems EnzyFluo™ Acetaldehyde Assay Kit. For quantitatve determination of acetaldehyde and evaluations of drug effects on its metabolism.

For quantitatve determination of acetaldehyde and evaluations of drug effects on its metabolism. Key Features: Fast and sensitive. Linear detection range (50µL sample): 0.5 µM to 60 µM acetaldehyde in 96-well plate assay. High-throughput. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. Convenient. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL530/585nm. Samples: Biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice). Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.5 µM.

Bioassay Systems EnzyLight™ ADP Assay Kit. For rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of drug effects on ADP metabolism.

For rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of drug effects on ADP metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ADP can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence; Samples: Cells etc; Size: 100 tests.

Bioassay Systems EnzyChrom™ Adipolysis Assay Kit. For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.

For quantitative assay of adipolysis and evaluation of drug effects on adipolysis. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.92 to 100 µg/mL (10 to 1000 µM) glycerol for colorimetric assays and 0.2 to 5 µg/mL for fluorimetric assays. Rapid and convenient.The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates. Method: OD570nm, or FL530/585nm. Samples: Cell culture media. Species: All. Procedure: Assay takes 20 min. Kit size: 200 tests. Detection limit: 0.92 µg/mL.

Bioassay Systems EnzyChrom™ Kinase Assay Kit. Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors.

Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.01 U/L kinase can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature. Robust and amenable to HTS: Assay can tolerate up to 300 µM ATP and 10% dimethylsulfoxide (DMSO). Z factors of > 0.6 are routinely observed in 96/384-well plates. Can be readily automated on HTS liquid handling systems for tens of thousands of assays per day. Method: FL530/585nm. Samples: All kinases. Species: All. Procedure: Assay takes 10 min. Kit size: 400 tests. Detection limit: 0.01 U/L.

Bioassay Systems QuantiChrom™ Peroxidase Assay Kit. For quantitative determination of peroxidase enzyme activity.

For quantitative determination of peroxidase enzyme activity. Key Features: Fast and sensitive. Use as little as 10 µL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase. Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: OD, FL: 4, 0.8 U/L.
PEROXIDASES (EC number 1.11.1.x) catalyze the following oxidation-reduction reactions:
ROOR + electron donor (2 e-) + 2H+ → ROH + R'OH.

Bioassay Systems EnzyChrom™ Xanthine Oxidase Assay Kit. For quantitative determination of xanthine oxidase enzyme activity.

For quantitative determination of xanthine oxidase enzyme activity. Key Features: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 20 minute incubation: 0.03 to 25 U/L xanthine oxidase for colorimetric assays and 0.01 to 2.5 U/L for fluorimetric assays. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Method: OD570nm, FL530/585nm; Samples: Cell lysate, serum, and other biological samples; Size: 100 tests.

Bioassay Systems QuantiFluo™ Acid Phosphatase Assay Kit. Acid Phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples.)

For quantitative determination of acid phosphatase activity in biological samples. Key Features: Fast and sensitive. Linear detection range (20 µL sample): 0.008 to 10 U/L for a 30 minute reaction. High-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: FL360/450. Samples: Biological (e.g. serum) samples. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.008 U/L.