United States Biological Corporation

United States Biological Corporation Nematode Growth Medium (NGM), Powder, 500g

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58C water bath. N1000 solution needs to completely cool to the desired temperature (58C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58C, place flask(s) onto stir plate(s). Maintain temperature at 58C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

United States Biological Corporation DROP OUT MIX - URACIL

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)

United States Biological Corporation Ames Medium w/L-Glutamine, Powder, 50 L

Ames' medium was formulated to support retinal tissue in relatively short-term culture. Rabbit retina has been incubated in Ames' medium for over 2 days with its metabolism and electrical responses to light stimuli well maintained. This mixture is a medium of choice for maintaining central nervous system tissue in vitro.

United States Biological Corporation ZYMOLYASE 100T 25MG

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological Corporation Erythromycin A 9,11-Imino Ether | 161193-44-8 | 5 mg

Erythromycin A 9,11-Imino Ether | Purity: ≥95% | Mol Wt: 730.93 | 161193-44-8 | 5 mg

United States Biological Corporation Zymolyase 100T Concentrate, 1 mL

Zymolyase, produced by a submerged culture of Arthrobacter luteus, is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase.

United States Biological Corporation Ames Medium w/L-Glutamine (Powder), 100 L

Ames' medium was formulated to support retinal tissue in relatively short-term culture. Rabbit retina has been incubated in Ames' medium for over 2 days with its metabolism and electrical responses to light stimuli well maintained. This mixture is a medium of choice for maintaining central nervous system tissue in vitro.

United States Biological Corporation Dulbecco’s MEM Powder, 10 L

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

United States Biological Corporation Nematode Growth Medium, Lite (NGM Lite), Powder, 500g

A modified preparation of NGM for use in Nematode growth studies. Appearance: Light tan, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH (after autoclaving): 5.5-6.5

United States Biological Corporation Glycine Ultrapure, 56-40-6, MFCD00008131, 5kg

Molecular Formula C2H5NO2, Purity ≥99%, Molecular Weight 75.07, Solubility: Complete solubility in water; slight solubility in alcohol and ether. Synonyms: Aminoacetic acid; Glycocol; Aminoethanoic acid. Glycine is used in Tris-Glycine electrophoresis buffer formulations. This is a highly purified grade suitable for use in peptide synthesis. Special grade of Glycine used specifically for cell culture and Molecular Biology applications.

United States Biological Corporation PEPTONE 2.5 KG

Peptone is an enzymatic digest of protein used to cultivate non-fastidious organisms, producing low-level growth. Deficient in carbohydrates, cystine and tryptophan. Appearance: Light tan, homogenous, free flowing powder pH: 7.0 �0.5 Amino Nitrogen: 6% Solubility (2%): Light yellow, clear, complete Total Nitrogen: 10% Reducing Sugars: Negative Undigested Protein: Negative Proteoses: Negative Nitrites: Negative Tryptophan: Negative Ash: 10% Moisture: 10% Typical Amino Acid Profile: Ala 8.5%, Arg 7.8%, Asp 6.2%, Cys 0.2%, Glu 9.8%, Gly 19.4%, His 0.8%, Ile 1.4%, Leu 2.9%, Lys 3.8%, Met 1.0%, Phe 1.9%, Pro 12.2%, Ser 3.7%, Thr 1.8%, Trp 0.05%, Tyr 0.7%, Val 2.2% Growth Supporting Properties (Agar/ Broth Formulations): Satisfactory Certificate of Origin: The raw animal products and enzymes, porcine tissue, used in the manufacturing of Peptone is derived from meat materials originating in the USA. During processing of the product, a batch is heated to a minimum of 80 �C.

United States Biological Corporation YEAST NITRGEN BSE W/O AA 500GR

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Yeast Nitrogen Base without amino acids (YNB w/o AA), Y2025, is used for selecting yeasts based on amino acid and carbohydrate requirements. In Y2025, Ammonium Sulfate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells. Compared to full YNB medium, Y2025 lacks Histidine, Methionine and Trypthophan. In combination with €œDrop Out € medium supplement mixtures, Y2025 provides an excellent medium to cultivate and select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins.

United States Biological Corporation Nonidet-P40 substitute, 9036-19-5, 500mL

Reagent Grade, Molecular Weight 603, Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene.

United States Biological Corporation Erythromycin A 9,11-Imino Ether, 161193-44-8

Molecular Formula C37H66N2O12, Purity ≥95%, Molecular Weight 730.93, Melting Point >134ºC (dec.), Solubility: Chloroform (slightly), DMSO (Slightly) Methanol (slightly). Erythromycin A 9,11-Imino Ether is an impurity in the synthesis of Erythromycin, a macrolide antibiotic with broad spectrum of antibacterial activity.

United States Biological Corporation DMEM F12 W/OUT GLUCOSE 10L D

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.