United States Biological Corporation

United States Biological CorporationSupplier Diversity Partner 5-FLOUROOROTIC ACID 10 GRAM DFS Item

Useful in the identification and selection of the strains of Saccharomyces cerevisiae that contain the mutant ura3- gene. 5-FOA is toxic to yeast cells that can synthesize the enzyme orotidine-5-phosphate decarboxylase and are therefore unable to grow on 5-FOA-containing media. Alternate Nomenclature: FOA; 5-FOA; 5-Fluoroorotate; 5-FLUORO-4-PYRIMIDINECARBOXYLIC ACID; 5-FLUORO-1,2,3,6-TETRAHYDRO-2,6-DIOXO-(9CI); 1,2,3,6-TETRAHYDRO-2,6-DIOXO-5-FLUORO-4- PYRIMIDINECARBOXYLIC ACID; 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid; 5-Fluorouracil-4-carboxylic acid. ura3 Strain Selection Assay: 5-FOA is tested for the ability to inhibit growth of ura3+ strains of Saccharomyces cerevisiae. Appearance: Off-white to light yellow powder. Melting Point: ~258 �C FOA is stable at high heat (i.e. boiling and autoclave conditions.) Refer to United States Biological reference article on stability testing data. Useful in the identification and selection of the strains of Saccharomyces cerevisiae that contain the mutant ura3- gene. 5-FOA is toxic to yeast cells that can synthesize the enzyme orotidine-5-phosphate decarboxylase and are therefore unable to grow on 5-FOA-containing media. Alternate Nomenclature: FOA; 5-FOA; 5-Fluoroorotate; 5-FLUORO-4-PYRIMIDINECARBOXYLIC ACID; 5-FLUORO-1,2,3,6-TETRAHYDRO-2,6-DIOXO-(9CI); 1,2,3,6-TETRAHYDRO-2,6-DIOXO-5-FLUORO-4- PYRIMIDINECARBOXYLIC ACID; 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid; 5-Fluorouracil-4-carboxylic acid. ura3 Strain Selection Assay: 5-FOA is tested for the ability to inhibit growth of ura3+ strains of Saccharomyces cerevisiae. Appearance: Off-white to light yellow powder. Melting Point: ~258 �C FOA is stable at high heat (i.e. boiling and autoclave conditions.) Refer to United States Biological reference article on stability testing data.

United States Biological CorporationSupplier Diversity Partner NP40 SUBSTITUTE 100ML DFS Item

Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25C): ~246 Surface Tension (0.1% aqueous, 25C): ~30 Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25C): ~246 Surface Tension (0.1% aqueous, 25C): ~30

United States Biological CorporationSupplier Diversity Partner RAFFINOSE LOW GLUCOSE 500G DFS Item

Used as an alternate carbon source for wild-type yeast. Appearance: White, crystalline powder Solubility: Colorless, clear, complete after autoclaving Loss on Drying: 14-16% Optical Rotation (C=10, H2O): +105±2.0° Ash: ≤0.1% Glucose: ≤0.02% Microbiology: Total Bacteria: ≤1000cfu/g Yeast, Mold and Fungi: ≤100cfu/g Salmonella: Negative E. coli: Negative Stapylococcus: Negative Melting Point: 78-80°C Arsenic: ≤ 0.0001% Iron: ≤ 0.0005% Heavy Metals (Pb): ≤ 0.0005% Used as an alternate carbon source for wild-type yeast. Appearance: White, crystalline powder Solubility: Colorless, clear, complete after autoclaving Loss on Drying: 14-16% Optical Rotation (C=10, H2O): +105±2.0° Ash: ≤0.1% Glucose: ≤0.02% Microbiology: Total Bacteria: ≤1000cfu/g Yeast, Mold and Fungi: ≤100cfu/g Salmonella: Negative E. coli: Negative Stapylococcus: Negative Melting Point: 78-80°C Arsenic: ≤ 0.0001% Iron: ≤ 0.0005% Heavy Metals (Pb): ≤ 0.0005%

United States Biological CorporationSupplier Diversity Partner ANTI HEPARAN SULFATE 50UG DFS Item

Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) �-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS- (1->4)-�-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc (1->4)-�-D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region. Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) �-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS- (1->4)-�-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc (1->4)-�-D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region.

United States Biological CorporationSupplier Diversity Partner IPTG DIOXINE FREE 10G DFS Item

IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli. Solubility: Colorless, clear, complete Identity (IR): Conforms to structure pH (5%): 5.0-7.0 Dioxane: Not Detected Specific Rotation (C=1,H2O): -29° to -35° Melting Point: 109 -114.0°C Water (KF): ≤1.0% Blue-White Assay: Induces b-galactosidase in a pUC 18-containing strain. Storage and Stability: Powder is very stable at RT. Stable for 12 months after receipt. For long-term storage of reconstituted product, aliquot and store at -20°C. Further dilutions can be made in assay buffer. IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli. Solubility: Colorless, clear, complete Identity (IR): Conforms to structure pH (5%): 5.0-7.0 Dioxane: Not Detected Specific Rotation (C=1,H2O): -29° to -35° Melting Point: 109 -114.0°C Water (KF): ≤1.0% Blue-White Assay: Induces b-galactosidase in a pUC 18-containing strain. Storage and Stability: Powder is very stable at RT. Stable for 12 months after receipt. For long-term storage of reconstituted product, aliquot and store at -20°C. Further dilutions can be made in assay buffer.

United States Biological CorporationSupplier Diversity Partner CASAMINO ACID 1KG DFS Item

Casamino Acid; Light Tan; Homogenous; Free Flowing Powder; 1kg; (6%) 6 to 7 pH Casamino Acid; Light Tan; Homogenous; Free Flowing Powder; 1kg; (6%) 6 to 7 pH

United States Biological CorporationSupplier Diversity Partner 5 KG GLYCINE ULTRAPURE DFS Item

Glycine is used in Tris-Glycine electrophoresis buffer formulations. This is a highly purified grade suitable for use in peptide synthesis. Special grade of Glycine used specifically for cell culture and Molecular Biology applications. Solubility: Complete solubility in water; slight solubility in alcohol and ether. pH (1%): 5.9-6.5 Residue on Ignition: ≤ 0.1% Heavy Metals (Pb): ≤ 0.002% Chloride: ≤ 0.007% Sulfate: ≤ 0.0065% Identity (IR): Conforms to reference USP Loss on Drying (Dry at 105°C for 2 hrs.): ≤ 0.2% RNase: None Detected DNase: None Detected Protease: None Detected Storage and Stability: Glycine is stable at RT. Powder may be stored at 4°C for long term storage. Stable for 12 months after receipt at 4°C. Glycine is used in Tris-Glycine electrophoresis buffer formulations. This is a highly purified grade suitable for use in peptide synthesis. Special grade of Glycine used specifically for cell culture and Molecular Biology applications. Solubility: Complete solubility in water; slight solubility in alcohol and ether. pH (1%): 5.9-6.5 Residue on Ignition: ≤ 0.1% Heavy Metals (Pb): ≤ 0.002% Chloride: ≤ 0.007% Sulfate: ≤ 0.0065% Identity (IR): Conforms to reference USP Loss on Drying (Dry at 105°C for 2 hrs.): ≤ 0.2% RNase: None Detected DNase: None Detected Protease: None Detected Storage and Stability: Glycine is stable at RT. Powder may be stored at 4°C for long term storage. Stable for 12 months after receipt at 4°C.

United States Biological CorporationSupplier Diversity Partner NOURSEOTHRICIN (CLONAT) 1G DFS Item

Notch 1 is a transmembrane protein functioning in the development and determination of cell-fate (1). During maturation, the Notch molecule is cleaved by furin-like convertase at the extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the C-terminal Notch 1 fragment dissociates from its N-terminal counterpart and is further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus and activates Notch-related transcription. Applications: Suitable for use in Western Blot, Immunoprecipitation, and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4�C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4�C for short-term only. Notch 1 is a transmembrane protein functioning in the development and determination of cell-fate (1). During maturation, the Notch molecule is cleaved by furin-like convertase at the extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the C-terminal Notch 1 fragment dissociates from its N-terminal counterpart and is further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus and activates Notch-related transcription. Applications: Suitable for use in Western Blot, Immunoprecipitation, and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4�C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4�C for short-term only.

United States Biological CorporationSupplier Diversity Partner DMEM F12 W/OUT GLUCOSE 10L D DFS Item

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells. Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

United States Biological CorporationSupplier Diversity Partner DROP OUT BASE/GALACTUSE/100GR DFS Item

Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (DO, Dropout, Powder) Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (DO, Dropout, Powder)

United States Biological CorporationSupplier Diversity Partner NP40 500ML DFS Item

Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25): ~246 Surface Tension (0.1% aqueous, 25C): ~30 Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25): ~246 Surface Tension (0.1% aqueous, 25C): ~30

United States Biological CorporationSupplier Diversity Partner PROTEOSE PEPTONENO3(PWDER)500G DFS Item

Proteose Peptone, 3 Agar; United States Biological Corporation; Light beige, homogeneous, free-flowing powder, Solubility: Clear to sl. hazy, amber, complete w/heating; Moisture: 5% Ash: 12% Amino Nitrogen: 6% Total Nitrogen: 15% pH Proteose Peptone, 3 Agar; United States Biological Corporation; Light beige, homogeneous, free-flowing powder, Solubility: Clear to sl. hazy, amber, complete w/heating; Moisture: 5% Ash: 12% Amino Nitrogen: 6% Total Nitrogen: 15% pH

United States Biological CorporationSupplier Diversity Partner ZYMOLYASE 100T 25MG DFS Item

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological CorporationSupplier Diversity Partner BACTO YEAST EXTRACT 500G DFS Item

Aqueous extract of brewers yeast with an exceptionally high vitamin content. Selected for greater solubility and clarity. Free amino acid content of approximately 25%. Yeast Extract is produced from autolyzed yeast cells and is very soluble in water. It is used as an enrichment in a large number of culture media for general bacteriology and in media for sterility according to the USP. Because of its high content of carbohydrates, Yeast Extract should not be used in fermentation studies. Typical Amino Acid Profile: Ala 42%, Arg 27%, Asp 59%, Cys 5%, Glu 105%, Gly 27%, His 12%, Ile 28%, Leu 42%, Lys 45%, Met 9%, Phe 24%, Pro 21%, Ser 27%, Thr 28%, Trp 6%, Tyr 21%, Val 36% Appearance: Light yellow to medium tan, homogeneous, free flowing powder Solubility: Light yellow to amber, clear, complete pH: 6.5 � 0.5 Ash: 17% Salt: 0.5% Nitrogen (Amino): 5% Nitrogen (AN/TN): 36% Microbiological Testing: Salmonella, Standard Plate Count, Coliform (MPN/g), Viable Yeast & Mold. Aqueous extract of brewers yeast with an exceptionally high vitamin content. Selected for greater solubility and clarity. Free amino acid content of approximately 25%. Yeast Extract is produced from autolyzed yeast cells and is very soluble in water. It is used as an enrichment in a large number of culture media for general bacteriology and in media for sterility according to the USP. Because of its high content of carbohydrates, Yeast Extract should not be used in fermentation studies. Typical Amino Acid Profile: Ala 42%, Arg 27%, Asp 59%, Cys 5%, Glu 105%, Gly 27%, His 12%, Ile 28%, Leu 42%, Lys 45%, Met 9%, Phe 24%, Pro 21%, Ser 27%, Thr 28%, Trp 6%, Tyr 21%, Val 36% Appearance: Light yellow to medium tan, homogeneous, free flowing powder Solubility: Light yellow to amber, clear, complete pH: 6.5 � 0.5 Ash: 17% Salt: 0.5% Nitrogen (Amino): 5% Nitrogen (AN/TN): 36% Microbiological Testing: Salmonella, Standard Plate Count, Coliform (MPN/g), Viable Yeast & Mold.

United States Biological CorporationSupplier Diversity Partner NUCLEASE P-1 100 UNITS DFS Item

Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase) from Penicillium citrinum, a zinc dependent glyco-enzyme consisting of 270 amino acid residues, hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. Nuclease P1 is capable of hydrolyzing single stranded DNA and RNA completely to the level of mononucleoside 5-monophosphates. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0. Activity: > 300U/mg protein using RNA substrate Unit Definition: One unit of nuclease activity is defined as the amount of enzyme that produces 1umole of acid soluble nucleotides from RNA based on E = 10,600 for RNA hydrolysates per minute at 37 �C, pH 5.3. Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase) from Penicillium citrinum, a zinc dependent glyco-enzyme consisting of 270 amino acid residues, hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. Nuclease P1 is capable of hydrolyzing single stranded DNA and RNA completely to the level of mononucleoside 5-monophosphates. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0. Activity: > 300U/mg protein using RNA substrate Unit Definition: One unit of nuclease activity is defined as the amount of enzyme that produces 1umole of acid soluble nucleotides from RNA based on E = 10,600 for RNA hydrolysates per minute at 37 �C, pH 5.3.

United States Biological CorporationSupplier Diversity Partner NEMATODE GROWTH MEDIA 500G DFS Item

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58�C water bath. N1000 solution needs to completely cool to the desired temperature (58�C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58�C, place flask(s) onto stir plate(s). Maintain temperature at 58�C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution. A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58�C water bath. N1000 solution needs to completely cool to the desired temperature (58�C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58�C, place flask(s) onto stir plate(s). Maintain temperature at 58�C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

United States Biological CorporationSupplier Diversity Partner PEPTONE 2.5 KG DFS Item

Peptone is an enzymatic digest of protein used to cultivate non-fastidious organisms, producing low-level growth. Deficient in carbohydrates, cystine and tryptophan. Appearance: Light tan, homogenous, free flowing powder pH: 7.0 �0.5 Amino Nitrogen: 6% Solubility (2%): Light yellow, clear, complete Total Nitrogen: 10% Reducing Sugars: Negative Undigested Protein: Negative Proteoses: Negative Nitrites: Negative Tryptophan: Negative Ash: 10% Moisture: 10% Typical Amino Acid Profile: Ala 8.5%, Arg 7.8%, Asp 6.2%, Cys 0.2%, Glu 9.8%, Gly 19.4%, His 0.8%, Ile 1.4%, Leu 2.9%, Lys 3.8%, Met 1.0%, Phe 1.9%, Pro 12.2%, Ser 3.7%, Thr 1.8%, Trp 0.05%, Tyr 0.7%, Val 2.2% Growth Supporting Properties (Agar/ Broth Formulations): Satisfactory Certificate of Origin: The raw animal products and enzymes, porcine tissue, used in the manufacturing of Peptone is derived from meat materials originating in the USA. During processing of the product, a batch is heated to a minimum of 80 �C. Peptone is an enzymatic digest of protein used to cultivate non-fastidious organisms, producing low-level growth. Deficient in carbohydrates, cystine and tryptophan. Appearance: Light tan, homogenous, free flowing powder pH: 7.0 �0.5 Amino Nitrogen: 6% Solubility (2%): Light yellow, clear, complete Total Nitrogen: 10% Reducing Sugars: Negative Undigested Protein: Negative Proteoses: Negative Nitrites: Negative Tryptophan: Negative Ash: 10% Moisture: 10% Typical Amino Acid Profile: Ala 8.5%, Arg 7.8%, Asp 6.2%, Cys 0.2%, Glu 9.8%, Gly 19.4%, His 0.8%, Ile 1.4%, Leu 2.9%, Lys 3.8%, Met 1.0%, Phe 1.9%, Pro 12.2%, Ser 3.7%, Thr 1.8%, Trp 0.05%, Tyr 0.7%, Val 2.2% Growth Supporting Properties (Agar/ Broth Formulations): Satisfactory Certificate of Origin: The raw animal products and enzymes, porcine tissue, used in the manufacturing of Peptone is derived from meat materials originating in the USA. During processing of the product, a batch is heated to a minimum of 80 �C.

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