United States Biological Corporation

United States Biological Corporation MYELIN BASIC PROTEIN 1MG DFS Item

NC0431731 Myelin Basic Protein, Human Brain, lyophilized (MBP) E Myelin Basic Protein, Human Brain, lyophilized (MBP) E

United States Biological Corporation NUCLEASE P-1 100 UNITS DFS Item

NC9308158 Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase) from Penicillium citrinum, a zinc dependent glyco-enzyme consisting of 270 amino acid residues, hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. Nuclease P1 is capable of hydrolyzing single stranded DNA and RNA completely to the level of mononucleoside 5-monophosphates. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0. Activity: > 300U/mg protein using RNA substrate Unit Definition: One unit of nuclease activity is defined as the amount of enzyme that produces 1umole of acid soluble nucleotides from RNA based on E = 10,600 for RNA hydrolysates per minute at 37 �C, pH 5.3. Nuclease P1 (Nuclease 5'-Nucleotidehydrolase, 3'-Phosphohydrolase) from Penicillium citrinum, a zinc dependent glyco-enzyme consisting of 270 amino acid residues, hydrolyzes both 3'-5'-phosphodiester bonds in RNA and heat denatured DNA and 3'-phosphomonoester bonds in mono- and oligonucleotides terminated by 3'-phosphate without base specificity. Nuclease P1 is capable of hydrolyzing single stranded DNA and RNA completely to the level of mononucleoside 5-monophosphates. The enzyme does not attack double-stranded nucleic acids, especially in the presence of more than 400mM of sodium chloride at pH 6.0. Activity: > 300U/mg protein using RNA substrate Unit Definition: One unit of nuclease activity is defined as the amount of enzyme that produces 1umole of acid soluble nucleotides from RNA based on E = 10,600 for RNA hydrolysates per minute at 37 �C, pH 5.3.

United States Biological Corporation NOURSEOTHRICIN (CLONAT) 1G DFS Item

NC0861695 Notch 1 is a transmembrane protein functioning in the development and determination of cell-fate (1). During maturation, the Notch molecule is cleaved by furin-like convertase at the extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the C-terminal Notch 1 fragment dissociates from its N-terminal counterpart and is further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus and activates Notch-related transcription. Applications: Suitable for use in Western Blot, Immunoprecipitation, and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4�C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4�C for short-term only. Notch 1 is a transmembrane protein functioning in the development and determination of cell-fate (1). During maturation, the Notch molecule is cleaved by furin-like convertase at the extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the C-terminal Notch 1 fragment dissociates from its N-terminal counterpart and is further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus and activates Notch-related transcription. Applications: Suitable for use in Western Blot, Immunoprecipitation, and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4�C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4�C for short-term only.

United States Biological Corporation DMEM F-12 W/O AMINO ACIDS DFS Item

NC1301018 DMEM F-12 W/O AMINO ACIDS DMEM F-12 W/O AMINO ACIDS

United States Biological Corporation AGAR, USP 2.5KG DFS Item

NC0014269 Bacteriological agar is a highly purified extract of select Gelidium marine algae, forming part of the cell wall, that gives excellent growth characteristics used in a wide variety of microbiological applications. Agar is a phycocolloid, a complex mixture of several sugar compounds that retains a gelatinous structure at RT after boiling. Bacteriological agar is purified by successive washings to remove or extensively reduce chemical contaminants, color and salts. After autoclaving, agar is light amber and free of particulates, solidifying into a clear, firm gel. Agar is suitable for all molecular biology applications (i.e. bacterial and phage growth, M13 and Lambda). It is superior to standard microbiology grade agars. Appearance: White to light tan, homogenous, free flowing powder. Solubility: Light tan, clear, complete after autoclaving Moisture: 15% Ash: 6% pH (1.5%, post autoclaving): 7.2 � 1.5 Gel Strength (1.5%): 600-1200g/cm2 Gel Point (1.5%): 34-38 �C Heavy Metals. Bacteriological agar is a highly purified extract of select Gelidium marine algae, forming part of the cell wall, that gives excellent growth characteristics used in a wide variety of microbiological applications. Agar is a phycocolloid, a complex mixture of several sugar compounds that retains a gelatinous structure at RT after boiling. Bacteriological agar is purified by successive washings to remove or extensively reduce chemical contaminants, color and salts. After autoclaving, agar is light amber and free of particulates, solidifying into a clear, firm gel. Agar is suitable for all molecular biology applications (i.e. bacterial and phage growth, M13 and Lambda). It is superior to standard microbiology grade agars. Appearance: White to light tan, homogenous, free flowing powder. Solubility: Light tan, clear, complete after autoclaving Moisture: 15% Ash: 6% pH (1.5%, post autoclaving): 7.2 � 1.5 Gel Strength (1.5%): 600-1200g/cm2 Gel Point (1.5%): 34-38 �C Heavy Metals.

United States Biological Corporation ANTI-GLYCERALDEHYDE-3PHOSPHATE DFS Item

NC0356794 Glyceraldehyde 3-Phosphate Dehydrogenase (GAPD, GAPDH) (Glycerol Free) Mab Mo xRb E B IC IF Glyceraldehyde 3-Phosphate Dehydrogenase (GAPD, GAPDH) (Glycerol Free) Mab Mo xRb E B IC IF

United States Biological Corporation DMEM F12 W/OUT GLUCOSE 25L D DFS Item

NC0202555 Dulbecco's MEM (DMEM) F-12 w/o Glucose Dulbecco's MEM (DMEM) F-12 w/o Glucose

United States Biological Corporation ANTI -SPINT2 HAI2 HAI-2 100UG DFS Item

NC0912920 Anti -SPINT2 (HAI2, HAI-2, Hepatocyte Growth Factor Activator Inhibitor Type 2) 100 ug. Anti -SPINT2 (HAI2, HAI-2, Hepatocyte Growth Factor Activator Inhibitor Type 2) 100 ug.

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