United States Biological Corporation

United States Biological Corporation DROP OUT MIX - URACIL

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)

United States Biological Corporation Nematode Growth Medium (NGM), Powder, 500g

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58C water bath. N1000 solution needs to completely cool to the desired temperature (58C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58C, place flask(s) onto stir plate(s). Maintain temperature at 58C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

United States Biological Corporation ZYMOLYASE 100T 25MG

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological Corporation Chick Embryo Extract, Ultrafiltrate, Liquid, 20 mL

Chick Embryo Extract, Ultrafiltrate is used as a supplement in some growth media formulations. It is prepared by blending 11-14 day old chick embryos in a balanced salt solution (3X embryo volume). The solution undergoes centrifugation to remove larger particles and debris. The supernatant is subjected to an ultrafiltration step with a 10kD MW cutoff to remove protein from the solution, producing a clear, amber liquid. This liquid is sterile-filtered.

United States Biological Corporation N1010 Nematode Growth Medium, 100X (Trace Metals Mix) (Powder)

N1010 Nematode Growth Medium, 100X (Trace Metals Mix) (Powder)

United States Biological Corporation Zymolyase 100T (Lyticase, Yeast Lytic Enzyme), 37340-57-1, 100mg

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological Corporation LB Broth Lennox, Powder, 2.5 kg

LB Lennox, a nutritionally rich medium, was developed by Lennox for the growth and maintenance of pure cultures of recombinant strains of E. coli. These strains are generally derived from E. coli K12 which are deficient in B vitamin production. LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950’s. These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins.

United States Biological Corporation MOU IMMUNOGLOB 100TEST

I7502-70B Mouse Anti-Immunoglobulin-Like Transcript 2 (ILT2, CD85j) (PE)The immunoglobulin-like transcript (ILT) family of activating and inhibitory type immunoreceptors are expressed on many leukocyte subsets and function in the regulation of immune responses (1-3). This family was also named leukocyte Ig-like receptors (LIR) and monocyte/macrophage Ig-like receptors (MIR). ILTs share significant homology with killer cell Ig-like receptors (KIR). The ILT genes are located on human chromosome 19q13.4 in the leukocyte receptor complex that also includes the genes encoding KIRs (4). With the exception of ILT6, which is a soluble molecule, all ILT family members are type I transmembrane proteins having two or four extracellular Ig-like domains (2, 3).

United States Biological Corporation ERTHRMYCN A9 11IMINO ETHR 1MG

Purity: ≥95% | Mol Wt: 730.93 | 161193-44-8 | 1 mg

United States Biological Corporation Mouse Anti-Phosphatase, Alkaline, Liver, Bone, Kidney (Akp2, ALPI, ALPL) 100ug

Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This antibody can be used to monitor the expression of the human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by Flow Cytometry.

United States Biological Corporation Mouse Anti-Streptavidin, Monoclonal, Mouse, IgG1, 1mg

Suitable for use in Immunohistochemistry, Flow Cytometry, ELISA, Immunocytochemistry and Western Blot. Other applications have not been tested.

United States Biological Corporation Nematode Growth Medium (NGM), Powder, 1 Kg

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58C water bath. N1000 solution needs to completely cool to the desired temperature (58C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58C, place flask(s) onto stir plate(s). Maintain temperature at 58C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

United States Biological Corporation YEAST NITROGEN BASE 500G

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Y2030 has the same formulation as YNB without Amino Acids except that ammonium sulfate as a source of nitrogen has been omitted to test the ability of yeast cells to assimilate different nitrogen sources (i.e. Potassium Nitrate). Application: For carbon assimilation tests in the characterization and identification of yeasts. Note: The addition of a carbon source like glucose, galactose or raffinose is required.

United States Biological Corporation RAFFINOSE LOW GLUCOSE 500G

Used as an alternate carbon source for wild-type yeast. Appearance: White, crystalline powder Solubility: Colorless, clear, complete after autoclaving Loss on Drying: 14-16% Optical Rotation (C=10, H2O): +105±2.0° Ash: ≤0.1% Glucose: ≤0.02% Microbiology: Total Bacteria: ≤1000cfu/g Yeast, Mold and Fungi: ≤100cfu/g Salmonella: Negative E. coli: Negative Stapylococcus: Negative Melting Point: 78-80°C Arsenic: ≤ 0.0001% Iron: ≤ 0.0005% Heavy Metals (Pb): ≤ 0.0005%

United States Biological Corporation NP40 SUBSTITUTE 100ML

Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25C): ~246 Surface Tension (0.1% aqueous, 25C): ~30