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United States Biological CorporationSupplier Diversity Partner Erythromycin A 9,11-Imino Ether, 161193-44-8 DFS Item

Molecular Formula C37H66N2O12, Purity ≥95%, Molecular Weight 730.93, Melting Point >134ºC (dec.), Solubility: Chloroform (slightly), DMSO (Slightly) Methanol (slightly). Erythromycin A 9,11-Imino Ether is an impurity in the synthesis of Erythromycin, a macrolide antibiotic with broad spectrum of antibacterial activity. Molecular Formula C37H66N2O12, Purity ≥95%, Molecular Weight 730.93, Melting Point >134ºC (dec.), Solubility: Chloroform (slightly), DMSO (Slightly) Methanol (slightly). Erythromycin A 9,11-Imino Ether is an impurity in the synthesis of Erythromycin, a macrolide antibiotic with broad spectrum of antibacterial activity.

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United States Biological CorporationSupplier Diversity Partner NP40 SUBSTITUTE 100ML DFS Item

Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25C): ~246 Surface Tension (0.1% aqueous, 25C): ~30 Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25C): ~246 Surface Tension (0.1% aqueous, 25C): ~30

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United States Biological CorporationSupplier Diversity Partner SERUM, GOAT , STERILE 100ML DFS Item

SERUM, GOAT , STERILE 100ML SERUM, GOAT , STERILE 100ML

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United States Biological CorporationSupplier Diversity Partner DMEM F12 W/OUT GLUCOSE 10L D DFS Item

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells. Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

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United States Biological CorporationSupplier Diversity Partner FATTY ACID LOW FREE BSA 250G DFS Item

Albumin, Bovine Serum, Fatty Acid Free, is our highest purity albumin specifically designed for use in all applications, including Immunology, Tissue Culture and Microbiology. Produced in the USA. Applications include Immunohematology, Serology, RIA, Blood Banking, ELISA Blocking and Protein Control formulation. Appearance: White to tan powder Albumin: 98% Fatty Acid: 0.05% IgG: None Detected pH: 6.7-7.3 Moisture: 5% Protease: None Detected Endotoxin: 3EU/mg Conductivity (10%): 3000uS Sterility: Filtered before lyophilization through a 0.2um membrane filter. Sodium (10%): As reported Chloride (10%): As reported Calcium (10%): As reported Iron (10%): As reported Vesicular Stomatitis Virus: Negative Bluetongue Virus: Negative Microbial Growth: 3CFU/ml Country of Origin: United States Certificate of Origin: All raw material used in the manufacturing of Bovine Serum Albumin, Fatty Acid Free is of United States origin and was manufactured in USDA-registered plants. Albumin, Bovine Serum, Fatty Acid Free, is our highest purity albumin specifically designed for use in all applications, including Immunology, Tissue Culture and Microbiology. Produced in the USA. Applications include Immunohematology, Serology, RIA, Blood Banking, ELISA Blocking and Protein Control formulation. Appearance: White to tan powder Albumin: 98% Fatty Acid: 0.05% IgG: None Detected pH: 6.7-7.3 Moisture: 5% Protease: None Detected Endotoxin: 3EU/mg Conductivity (10%): 3000uS Sterility: Filtered before lyophilization through a 0.2um membrane filter. Sodium (10%): As reported Chloride (10%): As reported Calcium (10%): As reported Iron (10%): As reported Vesicular Stomatitis Virus: Negative Bluetongue Virus: Negative Microbial Growth: 3CFU/ml Country of Origin: United States Certificate of Origin: All raw material used in the manufacturing of Bovine Serum Albumin, Fatty Acid Free is of United States origin and was manufactured in USDA-registered plants.

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United States Biological CorporationSupplier Diversity Partner Bacteriological agar, 2.5kg DFS Item

Bacteriological agar is a highly purified extract of select Gelidium marine algae, forming part of the cell wall, that gives excellent growth characteristics used in a wide variety of microbiological applications. Agar is a phycocolloid, a complex mixture of several sugar compounds that retains a gelatinous structure at RT after boiling. Bacteriological agar is purified by successive washings to remove or extensively reduce chemical contaminants, color and salts. After autoclaving, agar is light amber and free of particulates, solidifying into a clear, firm gel. Agar is suitable for all molecular biology applications (i.e. bacterial and phage growth, M13 and Lambda). It is superior to standard microbiology grade agars. Appearance: White to light tan, homogenous, free flowing powder. Solubility: Light tan, clear, complete after autoclaving Moisture: 15% Ash: 6% pH (1.5%, post autoclaving): 7.2 1.5 Gel Strength (1.5%): 600-1200g/cm2 Gel Point (1.5%): 34-38 C Heavy Metals. Bacteriological agar is a highly purified extract of select Gelidium marine algae, forming part of the cell wall, that gives excellent growth characteristics used in a wide variety of microbiological applications. Agar is a phycocolloid, a complex mixture of several sugar compounds that retains a gelatinous structure at RT after boiling. Bacteriological agar is purified by successive washings to remove or extensively reduce chemical contaminants, color and salts. After autoclaving, agar is light amber and free of particulates, solidifying into a clear, firm gel. Agar is suitable for all molecular biology applications (i.e. bacterial and phage growth, M13 and Lambda). It is superior to standard microbiology grade agars. Appearance: White to light tan, homogenous, free flowing powder. Solubility: Light tan, clear, complete after autoclaving Moisture: 15% Ash: 6% pH (1.5%, post autoclaving): 7.2 1.5 Gel Strength (1.5%): 600-1200g/cm2 Gel Point (1.5%): 34-38 C Heavy Metals.

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United States Biological CorporationSupplier Diversity Partner NGM POWDER 1 KG DFS Item

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58�C water bath. N1000 solution needs to completely cool to the desired temperature (58�C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58�C, place flask(s) onto stir plate(s). Maintain temperature at 58�C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution. A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58�C water bath. N1000 solution needs to completely cool to the desired temperature (58�C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58�C, place flask(s) onto stir plate(s). Maintain temperature at 58�C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

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United States Biological CorporationSupplier Diversity Partner DROP OUT MIX - URACIL DFS Item

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder) Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)

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United States Biological CorporationSupplier Diversity Partner LB BROTH LENNOX (POWDER) 500G DFS Item

LB Broth Lennox (Powder) 500g LB Broth Lennox (Powder) 500g

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United States Biological CorporationSupplier Diversity Partner DROP OUT BASE/GALACTUSE/100GR DFS Item

Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (DO, Dropout, Powder) Drop-out Mix Synthetic Minus Tryptophan w/o Yeast Nitrogen Base (DO, Dropout, Powder)

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United States Biological CorporationSupplier Diversity Partner L BROTH 'LURIA' BROTH 500G DFS Item

L-Broth (Luria Broth) is the standard nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, plasmid propagation, and protein expression. LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950s (1,2,3). �These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (4,5). �The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. �The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin. L-Broth (Luria Broth) is the standard nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, plasmid propagation, and protein expression. LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950s (1,2,3). �These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (4,5). �The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. �The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin.

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United States Biological CorporationSupplier Diversity Partner X/GAL 40MG PER ML/DMF 10ML EA DFS Item

Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white. Gene Cloning: In gene cloning, X-gal is used as a visual indication of whether a cell expresses a functional ²-galactosidase enzyme in a technique called blue/white screening. This method of screening is a convenient way of distinguishing a successful cloning product from other unsuccessful ones. The blue/white screening method relies on the principle of ±-complementation of the ²-galactosidase gene, where a fragment of the lacZ gene (lacZ±) in the plasmid can complement another mutant lacZ gene (lacZ”M15) in the cell. Both genes by themselves produce non-functional peptides, however, when expressed together, as when a plasmid containing lacZ± is transformed into a lacZ”M15 cells, they form a functional ²-galactosidase. Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white. Gene Cloning: In gene cloning, X-gal is used as a visual indication of whether a cell expresses a functional ²-galactosidase enzyme in a technique called blue/white screening. This method of screening is a convenient way of distinguishing a successful cloning product from other unsuccessful ones. The blue/white screening method relies on the principle of ±-complementation of the ²-galactosidase gene, where a fragment of the lacZ gene (lacZ±) in the plasmid can complement another mutant lacZ gene (lacZ”M15) in the cell. Both genes by themselves produce non-functional peptides, however, when expressed together, as when a plasmid containing lacZ± is transformed into a lacZ”M15 cells, they form a functional ²-galactosidase.

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United States Biological CorporationSupplier Diversity Partner ZYMOLYASE 100T 50MG DFS Item

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

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United States Biological CorporationSupplier Diversity Partner ZYMOLYASE 20T 1G DFS Item

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Source: Arthrobactor luteus Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 20u/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Source: Arthrobactor luteus Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 20u/mg

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United States Biological CorporationSupplier Diversity Partner RPMI 1640 MED POWDER 10L DFS Item

RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et. al. at Roswell Park Memorial Institute. Appearance: Pink, homogeneous, free flowing powder Solubility: Magenta, clear, complete pH: As reported Endotoxin: ≤1EU/ml RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et. al. at Roswell Park Memorial Institute. Appearance: Pink, homogeneous, free flowing powder Solubility: Magenta, clear, complete pH: As reported Endotoxin: ≤1EU/ml

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United States Biological CorporationSupplier Diversity Partner YEAST NITROGEN BASE 500G DFS Item

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Y2030 has the same formulation as YNB without Amino Acids except that ammonium sulfate as a source of nitrogen has been omitted to test the ability of yeast cells to assimilate different nitrogen sources (i.e. Potassium Nitrate). Application: For carbon assimilation tests in the characterization and identification of yeasts. Note: The addition of a carbon source like glucose, galactose or raffinose is required. Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Y2030 has the same formulation as YNB without Amino Acids except that ammonium sulfate as a source of nitrogen has been omitted to test the ability of yeast cells to assimilate different nitrogen sources (i.e. Potassium Nitrate). Application: For carbon assimilation tests in the characterization and identification of yeasts. Note: The addition of a carbon source like glucose, galactose or raffinose is required.

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United States Biological CorporationSupplier Diversity Partner NEMATODE GROWTH MEDIA 500G DFS Item

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58�C water bath. N1000 solution needs to completely cool to the desired temperature (58�C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58�C, place flask(s) onto stir plate(s). Maintain temperature at 58�C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution. A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58�C water bath. N1000 solution needs to completely cool to the desired temperature (58�C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58�C, place flask(s) onto stir plate(s). Maintain temperature at 58�C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

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United States Biological CorporationSupplier Diversity Partner ZYMOLYASE 100T 25MG DFS Item

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

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United States Biological CorporationSupplier Diversity Partner ZYMOLYASE 20T DFS Item

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Source: Arthrobactor luteus Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 20u/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Source: Arthrobactor luteus Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 20u/mg

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United States Biological CorporationSupplier Diversity Partner BSA 100G DFS Item

Albumin, Bovine Serum, Fatty Acid Free, is our highest purity albumin specifically designed for use in all applications, including Immunology, Tissue Culture and Microbiology. Produced in the USA. Applications include Immunohematology, Serology, RIA, Blood Banking, ELISA Blocking and Protein Control formulation. Appearance: White to tan powder Albumin: 98% Fatty Acid: 0.05% IgG: None Detected pH: 6.7-7.3 Moisture: 5% Protease: None Detected Endotoxin: 3EU/mg Conductivity (10%): 3000uS Sterility: Filtered before lyophilization through a 0.2um membrane filter. Sodium (10%): As reported Chloride (10%): As reported Calcium (10%): As reported Iron (10%): As reported Vesicular Stomatitis Virus: Negative Bluetongue Virus: Negative Microbial Growth: 3CFU/ml Country of Origin: United States Certificate of Origin: All raw material used in the manufacturing of Bovine Serum Albumin, Fatty Acid Free is of United States origin and was manufactured in USDA-registered plants. Albumin, Bovine Serum, Fatty Acid Free, is our highest purity albumin specifically designed for use in all applications, including Immunology, Tissue Culture and Microbiology. Produced in the USA. Applications include Immunohematology, Serology, RIA, Blood Banking, ELISA Blocking and Protein Control formulation. Appearance: White to tan powder Albumin: 98% Fatty Acid: 0.05% IgG: None Detected pH: 6.7-7.3 Moisture: 5% Protease: None Detected Endotoxin: 3EU/mg Conductivity (10%): 3000uS Sterility: Filtered before lyophilization through a 0.2um membrane filter. Sodium (10%): As reported Chloride (10%): As reported Calcium (10%): As reported Iron (10%): As reported Vesicular Stomatitis Virus: Negative Bluetongue Virus: Negative Microbial Growth: 3CFU/ml Country of Origin: United States Certificate of Origin: All raw material used in the manufacturing of Bovine Serum Albumin, Fatty Acid Free is of United States origin and was manufactured in USDA-registered plants.

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United States Biological CorporationSupplier Diversity Partner E2205 EDINBURGH MIN MEDIA 500G DFS Item

Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. EMM2 is used for vegetative growth. Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. EMM2 is used for vegetative growth.

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United States Biological CorporationSupplier Diversity Partner PEPTONE 2.5 KG DFS Item

Peptone is an enzymatic digest of protein used to cultivate non-fastidious organisms, producing low-level growth. Deficient in carbohydrates, cystine and tryptophan. Appearance: Light tan, homogenous, free flowing powder pH: 7.0 �0.5 Amino Nitrogen: 6% Solubility (2%): Light yellow, clear, complete Total Nitrogen: 10% Reducing Sugars: Negative Undigested Protein: Negative Proteoses: Negative Nitrites: Negative Tryptophan: Negative Ash: 10% Moisture: 10% Typical Amino Acid Profile: Ala 8.5%, Arg 7.8%, Asp 6.2%, Cys 0.2%, Glu 9.8%, Gly 19.4%, His 0.8%, Ile 1.4%, Leu 2.9%, Lys 3.8%, Met 1.0%, Phe 1.9%, Pro 12.2%, Ser 3.7%, Thr 1.8%, Trp 0.05%, Tyr 0.7%, Val 2.2% Growth Supporting Properties (Agar/ Broth Formulations): Satisfactory Certificate of Origin: The raw animal products and enzymes, porcine tissue, used in the manufacturing of Peptone is derived from meat materials originating in the USA. During processing of the product, a batch is heated to a minimum of 80 �C. Peptone is an enzymatic digest of protein used to cultivate non-fastidious organisms, producing low-level growth. Deficient in carbohydrates, cystine and tryptophan. Appearance: Light tan, homogenous, free flowing powder pH: 7.0 �0.5 Amino Nitrogen: 6% Solubility (2%): Light yellow, clear, complete Total Nitrogen: 10% Reducing Sugars: Negative Undigested Protein: Negative Proteoses: Negative Nitrites: Negative Tryptophan: Negative Ash: 10% Moisture: 10% Typical Amino Acid Profile: Ala 8.5%, Arg 7.8%, Asp 6.2%, Cys 0.2%, Glu 9.8%, Gly 19.4%, His 0.8%, Ile 1.4%, Leu 2.9%, Lys 3.8%, Met 1.0%, Phe 1.9%, Pro 12.2%, Ser 3.7%, Thr 1.8%, Trp 0.05%, Tyr 0.7%, Val 2.2% Growth Supporting Properties (Agar/ Broth Formulations): Satisfactory Certificate of Origin: The raw animal products and enzymes, porcine tissue, used in the manufacturing of Peptone is derived from meat materials originating in the USA. During processing of the product, a batch is heated to a minimum of 80 �C.

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