United States Biological Corporation

United States Biological CorporationSupplier Diversity Partner 5-FLOUROOROTIC ACID 10 GRAM DFS Item

Useful in the identification and selection of the strains of Saccharomyces cerevisiae that contain the mutant ura3- gene. 5-FOA is toxic to yeast cells that can synthesize the enzyme orotidine-5-phosphate decarboxylase and are therefore unable to grow on 5-FOA-containing media. Alternate Nomenclature: FOA; 5-FOA; 5-Fluoroorotate; 5-FLUORO-4-PYRIMIDINECARBOXYLIC ACID; 5-FLUORO-1,2,3,6-TETRAHYDRO-2,6-DIOXO-(9CI); 1,2,3,6-TETRAHYDRO-2,6-DIOXO-5-FLUORO-4- PYRIMIDINECARBOXYLIC ACID; 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid; 5-Fluorouracil-4-carboxylic acid. ura3 Strain Selection Assay: 5-FOA is tested for the ability to inhibit growth of ura3+ strains of Saccharomyces cerevisiae. Appearance: Off-white to light yellow powder. Melting Point: ~258 �C FOA is stable at high heat (i.e. boiling and autoclave conditions.) Refer to United States Biological reference article on stability testing data. Useful in the identification and selection of the strains of Saccharomyces cerevisiae that contain the mutant ura3- gene. 5-FOA is toxic to yeast cells that can synthesize the enzyme orotidine-5-phosphate decarboxylase and are therefore unable to grow on 5-FOA-containing media. Alternate Nomenclature: FOA; 5-FOA; 5-Fluoroorotate; 5-FLUORO-4-PYRIMIDINECARBOXYLIC ACID; 5-FLUORO-1,2,3,6-TETRAHYDRO-2,6-DIOXO-(9CI); 1,2,3,6-TETRAHYDRO-2,6-DIOXO-5-FLUORO-4- PYRIMIDINECARBOXYLIC ACID; 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid; 5-Fluorouracil-4-carboxylic acid. ura3 Strain Selection Assay: 5-FOA is tested for the ability to inhibit growth of ura3+ strains of Saccharomyces cerevisiae. Appearance: Off-white to light yellow powder. Melting Point: ~258 �C FOA is stable at high heat (i.e. boiling and autoclave conditions.) Refer to United States Biological reference article on stability testing data.

United States Biological CorporationSupplier Diversity Partner NP40 SUBSTITUTE 100ML DFS Item

Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25C): ~246 Surface Tension (0.1% aqueous, 25C): ~30 Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25C): ~246 Surface Tension (0.1% aqueous, 25C): ~30

United States Biological CorporationSupplier Diversity Partner DROSOPHILA GRADE AGAR 2.5KG DFS Item

Gelidium based Drosophila agar is an inexpensive growth medium especially suited to the growth and nutrient requirements of Drosophila without the expense of bacteriological grades. This is a food grade agar which offers excellent clarity. Appearance: White to light tan, homogenous, free flowing powder. Melting Point (1.5%): 82-88°C pH (1.5%): 6.0-8.0 Water: ≤11% Ash: ≤6.5% Gel Point (1.5%): 34-38°C Gel Strength (1.5%): 600-1100g/cm2 Identification A: Passes Identification B: Passes Foreign Insoluble Matter: ≤10% Foreign Organic Matter: ≤1% Foreign Starch: None Detected Gelatin: None Detected Water Absorption: ≤75ml Acid-insoluble Ash: ≤0.5% Arsenic: ≤0.0003% Lead: ≤0.001% Heavy Metals (Pb): ≤0.004% Microbiological Analysis: Total Plate Count: ≤500cfu/g Coliforms: ≤3cfu/g E. coli: 0cfu/g Salmonella: 0cfu/g Yeast and Mold: ≤200cfu/g Gelidium based Drosophila agar is an inexpensive growth medium especially suited to the growth and nutrient requirements of Drosophila without the expense of bacteriological grades. This is a food grade agar which offers excellent clarity. Appearance: White to light tan, homogenous, free flowing powder. Melting Point (1.5%): 82-88°C pH (1.5%): 6.0-8.0 Water: ≤11% Ash: ≤6.5% Gel Point (1.5%): 34-38°C Gel Strength (1.5%): 600-1100g/cm2 Identification A: Passes Identification B: Passes Foreign Insoluble Matter: ≤10% Foreign Organic Matter: ≤1% Foreign Starch: None Detected Gelatin: None Detected Water Absorption: ≤75ml Acid-insoluble Ash: ≤0.5% Arsenic: ≤0.0003% Lead: ≤0.001% Heavy Metals (Pb): ≤0.004% Microbiological Analysis: Total Plate Count: ≤500cfu/g Coliforms: ≤3cfu/g E. coli: 0cfu/g Salmonella: 0cfu/g Yeast and Mold: ≤200cfu/g

United States Biological CorporationSupplier Diversity Partner IPTG DIOXINE FREE 10G DFS Item

IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli. Solubility: Colorless, clear, complete Identity (IR): Conforms to structure pH (5%): 5.0-7.0 Dioxane: Not Detected Specific Rotation (C=1,H2O): -29° to -35° Melting Point: 109 -114.0°C Water (KF): ≤1.0% Blue-White Assay: Induces b-galactosidase in a pUC 18-containing strain. Storage and Stability: Powder is very stable at RT. Stable for 12 months after receipt. For long-term storage of reconstituted product, aliquot and store at -20°C. Further dilutions can be made in assay buffer. IPTG is a carbohydrate used to induce b-galactosidase for the selection of recombinant plasmids. Used to select for lac Y mutants and to induce the lac operon in E. coli. IPTG will also induce the cellular content of lactose permease. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in E. coli. Solubility: Colorless, clear, complete Identity (IR): Conforms to structure pH (5%): 5.0-7.0 Dioxane: Not Detected Specific Rotation (C=1,H2O): -29° to -35° Melting Point: 109 -114.0°C Water (KF): ≤1.0% Blue-White Assay: Induces b-galactosidase in a pUC 18-containing strain. Storage and Stability: Powder is very stable at RT. Stable for 12 months after receipt. For long-term storage of reconstituted product, aliquot and store at -20°C. Further dilutions can be made in assay buffer.

United States Biological CorporationSupplier Diversity Partner ANTI HEPARAN SULFATE 50UG DFS Item

Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) �-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS- (1->4)-�-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc (1->4)-�-D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region. Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) �-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS- (1->4)-�-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc (1->4)-�-D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region.

United States Biological CorporationSupplier Diversity Partner RAFFINOSE LOW GLUCOSE 500G DFS Item

Used as an alternate carbon source for wild-type yeast. Appearance: White, crystalline powder Solubility: Colorless, clear, complete after autoclaving Loss on Drying: 14-16% Optical Rotation (C=10, H2O): +105±2.0° Ash: ≤0.1% Glucose: ≤0.02% Microbiology: Total Bacteria: ≤1000cfu/g Yeast, Mold and Fungi: ≤100cfu/g Salmonella: Negative E. coli: Negative Stapylococcus: Negative Melting Point: 78-80°C Arsenic: ≤ 0.0001% Iron: ≤ 0.0005% Heavy Metals (Pb): ≤ 0.0005% Used as an alternate carbon source for wild-type yeast. Appearance: White, crystalline powder Solubility: Colorless, clear, complete after autoclaving Loss on Drying: 14-16% Optical Rotation (C=10, H2O): +105±2.0° Ash: ≤0.1% Glucose: ≤0.02% Microbiology: Total Bacteria: ≤1000cfu/g Yeast, Mold and Fungi: ≤100cfu/g Salmonella: Negative E. coli: Negative Stapylococcus: Negative Melting Point: 78-80°C Arsenic: ≤ 0.0001% Iron: ≤ 0.0005% Heavy Metals (Pb): ≤ 0.0005%

United States Biological CorporationSupplier Diversity Partner ZYMOLYASE 100T 500MG DFS Item

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological CorporationSupplier Diversity Partner 5 KG GLYCINE ULTRAPURE DFS Item

Glycine is used in Tris-Glycine electrophoresis buffer formulations. This is a highly purified grade suitable for use in peptide synthesis. Special grade of Glycine used specifically for cell culture and Molecular Biology applications. Solubility: Complete solubility in water; slight solubility in alcohol and ether. pH (1%): 5.9-6.5 Residue on Ignition: ≤ 0.1% Heavy Metals (Pb): ≤ 0.002% Chloride: ≤ 0.007% Sulfate: ≤ 0.0065% Identity (IR): Conforms to reference USP Loss on Drying (Dry at 105°C for 2 hrs.): ≤ 0.2% RNase: None Detected DNase: None Detected Protease: None Detected Storage and Stability: Glycine is stable at RT. Powder may be stored at 4°C for long term storage. Stable for 12 months after receipt at 4°C. Glycine is used in Tris-Glycine electrophoresis buffer formulations. This is a highly purified grade suitable for use in peptide synthesis. Special grade of Glycine used specifically for cell culture and Molecular Biology applications. Solubility: Complete solubility in water; slight solubility in alcohol and ether. pH (1%): 5.9-6.5 Residue on Ignition: ≤ 0.1% Heavy Metals (Pb): ≤ 0.002% Chloride: ≤ 0.007% Sulfate: ≤ 0.0065% Identity (IR): Conforms to reference USP Loss on Drying (Dry at 105°C for 2 hrs.): ≤ 0.2% RNase: None Detected DNase: None Detected Protease: None Detected Storage and Stability: Glycine is stable at RT. Powder may be stored at 4°C for long term storage. Stable for 12 months after receipt at 4°C.

United States Biological CorporationSupplier Diversity Partner PROTEOSE PEPTONENO3(PWDER)500G DFS Item

Proteose Peptone, 3 Agar; United States Biological Corporation; Light beige, homogeneous, free-flowing powder, Solubility: Clear to sl. hazy, amber, complete w/heating; Moisture: 5% Ash: 12% Amino Nitrogen: 6% Total Nitrogen: 15% pH Proteose Peptone, 3 Agar; United States Biological Corporation; Light beige, homogeneous, free-flowing powder, Solubility: Clear to sl. hazy, amber, complete w/heating; Moisture: 5% Ash: 12% Amino Nitrogen: 6% Total Nitrogen: 15% pH

United States Biological CorporationSupplier Diversity Partner TRIS BASE, ULTRAPURE, 5KG DFS Item

Tris Base Ultrapure (Encompass) Tris Base Ultrapure (Encompass)

United States Biological CorporationSupplier Diversity Partner RPMI 1640MED W/O AM 10L DFS Item

RPMI-1640 was developed by Moore, et al., at Roswell Park Memorial Institute, hence the acronym RPMI.�The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.�RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes.�RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay. RPMI-1640 was developed by Moore, et al., at Roswell Park Memorial Institute, hence the acronym RPMI.�The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.�RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes.�RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

United States Biological CorporationSupplier Diversity Partner ZYMOLYASE 20T 1G DFS Item

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Source: Arthrobactor luteus Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 20u/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Source: Arthrobactor luteus Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 20u/mg

United States Biological CorporationSupplier Diversity Partner D+ GLUCOSE ANHYDROUS DFS Item

Used as a carbohydrate source in cell culture and molecular biology. Appearance: White, crystalline powder Purity: ≥99.5% Identity: To pass USP Specific Rotation: +52.6° to +53.2° Loss on Drying: ≤0.2% Residue on Ignition: ≤0.1% Acidity, ml: To pass USP Starch: To pass USP Dextrin: To pass USP Chloride: ≤0.018% Sulfate: ≤0.025% Arsenic: ≤0.0001% Heavy Metals (Pb): ≤ 0.0005% Meets or exceeds USP specifications Certificate of Origin: USA Used as a carbohydrate source in cell culture and molecular biology. Appearance: White, crystalline powder Purity: ≥99.5% Identity: To pass USP Specific Rotation: +52.6° to +53.2° Loss on Drying: ≤0.2% Residue on Ignition: ≤0.1% Acidity, ml: To pass USP Starch: To pass USP Dextrin: To pass USP Chloride: ≤0.018% Sulfate: ≤0.025% Arsenic: ≤0.0001% Heavy Metals (Pb): ≤ 0.0005% Meets or exceeds USP specifications Certificate of Origin: USA

United States Biological CorporationSupplier Diversity Partner ZYMOLYASE 100T 100MG DFS Item

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological CorporationSupplier Diversity Partner YEAST NITRGEN BSE W/O AA 500GR DFS Item

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Yeast Nitrogen Base without amino acids (YNB w/o AA), Y2025, is used for selecting yeasts based on amino acid and carbohydrate requirements. In Y2025, Ammonium Sulfate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells. Compared to full YNB medium, Y2025 lacks Histidine, Methionine and Trypthophan. In combination with €œDrop Out € medium supplement mixtures, Y2025 provides an excellent medium to cultivate and select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins. Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Yeast Nitrogen Base without amino acids (YNB w/o AA), Y2025, is used for selecting yeasts based on amino acid and carbohydrate requirements. In Y2025, Ammonium Sulfate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells. Compared to full YNB medium, Y2025 lacks Histidine, Methionine and Trypthophan. In combination with €œDrop Out € medium supplement mixtures, Y2025 provides an excellent medium to cultivate and select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins.

United States Biological CorporationSupplier Diversity Partner ZYMOLYASE 100T 25MG DFS Item

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological CorporationSupplier Diversity Partner FATTY ACID LOW FREE BSA 250G DFS Item

Albumin, Bovine Serum, Fatty Acid Free, is our highest purity albumin specifically designed for use in all applications, including Immunology, Tissue Culture and Microbiology. Produced in the USA. Applications include Immunohematology, Serology, RIA, Blood Banking, ELISA Blocking and Protein Control formulation. Appearance: White to tan powder Albumin: 98% Fatty Acid: 0.05% IgG: None Detected pH: 6.7-7.3 Moisture: 5% Protease: None Detected Endotoxin: 3EU/mg Conductivity (10%): 3000uS Sterility: Filtered before lyophilization through a 0.2um membrane filter. Sodium (10%): As reported Chloride (10%): As reported Calcium (10%): As reported Iron (10%): As reported Vesicular Stomatitis Virus: Negative Bluetongue Virus: Negative Microbial Growth: 3CFU/ml Country of Origin: United States Certificate of Origin: All raw material used in the manufacturing of Bovine Serum Albumin, Fatty Acid Free is of United States origin and was manufactured in USDA-registered plants. Albumin, Bovine Serum, Fatty Acid Free, is our highest purity albumin specifically designed for use in all applications, including Immunology, Tissue Culture and Microbiology. Produced in the USA. Applications include Immunohematology, Serology, RIA, Blood Banking, ELISA Blocking and Protein Control formulation. Appearance: White to tan powder Albumin: 98% Fatty Acid: 0.05% IgG: None Detected pH: 6.7-7.3 Moisture: 5% Protease: None Detected Endotoxin: 3EU/mg Conductivity (10%): 3000uS Sterility: Filtered before lyophilization through a 0.2um membrane filter. Sodium (10%): As reported Chloride (10%): As reported Calcium (10%): As reported Iron (10%): As reported Vesicular Stomatitis Virus: Negative Bluetongue Virus: Negative Microbial Growth: 3CFU/ml Country of Origin: United States Certificate of Origin: All raw material used in the manufacturing of Bovine Serum Albumin, Fatty Acid Free is of United States origin and was manufactured in USDA-registered plants.

United States Biological CorporationSupplier Diversity Partner NGM POWDER 1 KG DFS Item

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58�C water bath. N1000 solution needs to completely cool to the desired temperature (58�C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58�C, place flask(s) onto stir plate(s). Maintain temperature at 58�C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution. A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58�C water bath. N1000 solution needs to completely cool to the desired temperature (58�C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58�C, place flask(s) onto stir plate(s). Maintain temperature at 58�C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

United States Biological CorporationSupplier Diversity Partner DROP OUT MIX - URACIL DFS Item

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder) Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)

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