United States Biological Corporation

United States Biological Corporation Nematode Growth Medium (NGM), Powder, 500g

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58C water bath. N1000 solution needs to completely cool to the desired temperature (58C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58C, place flask(s) onto stir plate(s). Maintain temperature at 58C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

United States Biological Corporation Chick Embryo Extract, Ultrafiltrate, Liquid, 20 mL

Chick Embryo Extract, Ultrafiltrate is used as a supplement in some growth media formulations. It is prepared by blending 11-14 day old chick embryos in a balanced salt solution (3X embryo volume). The solution undergoes centrifugation to remove larger particles and debris. The supernatant is subjected to an ultrafiltration step with a 10kD MW cutoff to remove protein from the solution, producing a clear, amber liquid. This liquid is sterile-filtered.

United States Biological Corporation N1010 Nematode Growth Medium, 100X (Trace Metals Mix) (Powder)

N1010 Nematode Growth Medium, 100X (Trace Metals Mix) (Powder)

United States Biological Corporation Zymolyase 100T (Lyticase, Yeast Lytic Enzyme), 37340-57-1, 100mg

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological Corporation Nematode Growth Medium, Lite (NGM Lite), Powder, 500g

A modified preparation of NGM for use in Nematode growth studies. Appearance: Light tan, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH (after autoclaving): 5.5-6.5

United States Biological Corporation X/GAL 40MG PER ML/DMF 10ML EA

Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white. Gene Cloning: In gene cloning, X-gal is used as a visual indication of whether a cell expresses a functional ²-galactosidase enzyme in a technique called blue/white screening. This method of screening is a convenient way of distinguishing a successful cloning product from other unsuccessful ones. The blue/white screening method relies on the principle of ±-complementation of the ²-galactosidase gene, where a fragment of the lacZ gene (lacZ±) in the plasmid can complement another mutant lacZ gene (lacZ”M15) in the cell. Both genes by themselves produce non-functional peptides, however, when expressed together, as when a plasmid containing lacZ± is transformed into a lacZ”M15 cells, they form a functional ²-galactosidase.

United States Biological Corporation BACTO YEAST EXTRACT 500G

Aqueous extract of brewers yeast with an exceptionally high vitamin content. Selected for greater solubility and clarity. Free amino acid content of approximately 25%. Yeast Extract is produced from autolyzed yeast cells and is very soluble in water. It is used as an enrichment in a large number of culture media for general bacteriology and in media for sterility according to the USP. Because of its high content of carbohydrates, Yeast Extract should not be used in fermentation studies. Typical Amino Acid Profile: Ala 42%, Arg 27%, Asp 59%, Cys 5%, Glu 105%, Gly 27%, His 12%, Ile 28%, Leu 42%, Lys 45%, Met 9%, Phe 24%, Pro 21%, Ser 27%, Thr 28%, Trp 6%, Tyr 21%, Val 36% Appearance: Light yellow to medium tan, homogeneous, free flowing powder Solubility: Light yellow to amber, clear, complete pH: 6.5 � 0.5 Ash: 17% Salt: 0.5% Nitrogen (Amino): 5% Nitrogen (AN/TN): 36% Microbiological Testing: Salmonella, Standard Plate Count, Coliform (MPN/g), Viable Yeast & Mold.

United States Biological Corporation ZYMOLYASE 100T 25MG

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

United States Biological Corporation DROP-OUT MIX COMP W/O YNB 100G

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)

United States Biological Corporation 5-Fluoroorotic Acid Monohydrate, 207291-81-4,220141-70-8,703-95-7,

Molecular Formula C5H3FN2O4.H2O, Molecular Biology Grade, Molecular Weight 192.1, Synonyms: FOA, 5-FOA, 5-Fluoroorotate, 5-fluoro-4-pyrimidinecarboxylic acid, 5-fluoro-1,2,3,6-tetrahydro-2,6-dioxo-(9CI), 1,2,3,6-tetrahydro-2,6-dioxo-5-fluoro-4-pyrimidinecarboxylic acid, 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid, 5-Fluorouracil-4-carboxylic acid. Useful in the identification and selection of the strains of Saccharomyces cerevisiae that contain the mutant ura3- gene. 5-FOA is toxic to yeast cells that can synthesize the enzyme orotidine-5’-phosphate decarboxylase and are therefore unable to grow on 5-FOA-containing media.

United States Biological Corporation Bacteriological agar, 2.5kg

Bacteriological agar is a highly purified extract of select Gelidium marine algae, forming part of the cell wall, that gives excellent growth characteristics used in a wide variety of microbiological applications. Agar is a phycocolloid, a complex mixture of several sugar compounds that retains a gelatinous structure at RT after boiling. Bacteriological agar is purified by successive washings to remove or extensively reduce chemical contaminants, color and salts. After autoclaving, agar is light amber and free of particulates, solidifying into a clear, firm gel. Agar is suitable for all molecular biology applications (i.e. bacterial and phage growth, M13 and Lambda). It is superior to standard microbiology grade agars. Appearance: White to light tan, homogenous, free flowing powder. Solubility: Light tan, clear, complete after autoclaving Moisture: 15% Ash: 6% pH (1.5%, post autoclaving): 7.2 1.5 Gel Strength (1.5%): 600-1200g/cm2 Gel Point (1.5%): 34-38 C Heavy Metals.

United States Biological Corporation Dulbecco’s MEM (DMEM) F-12 w/o Tryptophan (Powder) 10L

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

United States Biological Corporation 037167-PE Rabbit Anti-ITPRIPL1, NT (ITPRIPL1, KIAA1754L, Inositol 1,4,5-trisphosphate receptor-interacting protein-like 1) (PE)

ITPRIPL1 (inositol 1,4,5-triphosphate receptor-interacting protein-like 1), also known as KIAA1754L, is a 555 amino acid protein belonging to the ITPRIP family. ITPRIPL1 is a single-pass type I membrane protein expressed as two isoforms produced by alternative splicing events.

United States Biological Corporation Mouse Anti-CD209 (CD209 Antigen, C-type Lectin Domain Family 4 Member L, Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1, DC-SIGN, DC-SIGN1, CLEC4L, MGC129965) (MaxLight 490), 100ul

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

United States Biological Corporation L BROTH 'LURIA' BROTH 500G

L-Broth (Luria Broth) is the standard nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, plasmid propagation, and protein expression. LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950s (1,2,3). �These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (4,5). �The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. �The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin.