Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25C): ~246 Surface Tension (0.1% aqueous, 25C): ~30 Nonidet-P40 is an anhydrous liquid nonionic surface- active agent produced by the reaction of octyl phenol with 8.5-9.5 moles of ethylene oxide. Nonidet-P40 (NP-40) is a nonionic surfactant used in the isolation of membrane complexes. This product has been reformulated to be eco-friendly. The only observable differences are that the viscosity and handling characteristics are somewhat modified. Due to its nonionic structure, this product is compatible with anionic surfactants and is stable in the presence of acids, bases, and salts. It should not be mixed with concentrated oxidizing or reducing agents since the mixture of these compounds with organic compounds could form a potentially explosive mixture. Nonidet-P40 is an effective emulsifier for solvents such as xylene. General Specifications: Appearance: Colorless to pale yellow, clear, viscous liquid pH (1% aqueous): 5-7 Water: 0.50% Specific Gravity (25C): 1.065 Viscosity (cP, 25C): ~246 Surface Tension (0.1% aqueous, 25C): ~30

Useful in the identification and selection of the strains of Saccharomyces cerevisiae that contain the mutant ura3- gene. 5-FOA is toxic to yeast cells that can synthesize the enzyme orotidine-5-phosphate decarboxylase and are therefore unable to grow on 5-FOA-containing media. Alternate Nomenclature: FOA; 5-FOA; 5-Fluoroorotate; 5-FLUORO-4-PYRIMIDINECARBOXYLIC ACID; 5-FLUORO-1,2,3,6-TETRAHYDRO-2,6-DIOXO-(9CI); 1,2,3,6-TETRAHYDRO-2,6-DIOXO-5-FLUORO-4- PYRIMIDINECARBOXYLIC ACID; 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid; 5-Fluorouracil-4-carboxylic acid. ura3 Strain Selection Assay: 5-FOA is tested for the ability to inhibit growth of ura3+ strains of Saccharomyces cerevisiae. Appearance: Off-white to light yellow powder. Melting Point: ~258 �C FOA is stable at high heat (i.e. boiling and autoclave conditions.) Refer to United States Biological reference article on stability testing data. Useful in the identification and selection of the strains of Saccharomyces cerevisiae that contain the mutant ura3- gene. 5-FOA is toxic to yeast cells that can synthesize the enzyme orotidine-5-phosphate decarboxylase and are therefore unable to grow on 5-FOA-containing media. Alternate Nomenclature: FOA; 5-FOA; 5-Fluoroorotate; 5-FLUORO-4-PYRIMIDINECARBOXYLIC ACID; 5-FLUORO-1,2,3,6-TETRAHYDRO-2,6-DIOXO-(9CI); 1,2,3,6-TETRAHYDRO-2,6-DIOXO-5-FLUORO-4- PYRIMIDINECARBOXYLIC ACID; 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid; 5-Fluorouracil-4-carboxylic acid. ura3 Strain Selection Assay: 5-FOA is tested for the ability to inhibit growth of ura3+ strains of Saccharomyces cerevisiae. Appearance: Off-white to light yellow powder. Melting Point: ~258 �C FOA is stable at high heat (i.e. boiling and autoclave conditions.) Refer to United States Biological reference article on stability testing data.

Gelidium based Drosophila agar is an inexpensive growth medium especially suited to the growth and nutrient requirements of Drosophila without the expense of bacteriological grades. This is a food grade agar which offers excellent clarity. Appearance: White to light tan, homogenous, free flowing powder. Melting Point (1.5%): 82-88°C pH (1.5%): 6.0-8.0 Water: ≤11% Ash: ≤6.5% Gel Point (1.5%): 34-38°C Gel Strength (1.5%): 600-1100g/cm2 Identification A: Passes Identification B: Passes Foreign Insoluble Matter: ≤10% Foreign Organic Matter: ≤1% Foreign Starch: None Detected Gelatin: None Detected Water Absorption: ≤75ml Acid-insoluble Ash: ≤0.5% Arsenic: ≤0.0003% Lead: ≤0.001% Heavy Metals (Pb): ≤0.004% Microbiological Analysis: Total Plate Count: ≤500cfu/g Coliforms: ≤3cfu/g E. coli: 0cfu/g Salmonella: 0cfu/g Yeast and Mold: ≤200cfu/g Gelidium based Drosophila agar is an inexpensive growth medium especially suited to the growth and nutrient requirements of Drosophila without the expense of bacteriological grades. This is a food grade agar which offers excellent clarity. Appearance: White to light tan, homogenous, free flowing powder. Melting Point (1.5%): 82-88°C pH (1.5%): 6.0-8.0 Water: ≤11% Ash: ≤6.5% Gel Point (1.5%): 34-38°C Gel Strength (1.5%): 600-1100g/cm2 Identification A: Passes Identification B: Passes Foreign Insoluble Matter: ≤10% Foreign Organic Matter: ≤1% Foreign Starch: None Detected Gelatin: None Detected Water Absorption: ≤75ml Acid-insoluble Ash: ≤0.5% Arsenic: ≤0.0003% Lead: ≤0.001% Heavy Metals (Pb): ≤0.004% Microbiological Analysis: Total Plate Count: ≤500cfu/g Coliforms: ≤3cfu/g E. coli: 0cfu/g Salmonella: 0cfu/g Yeast and Mold: ≤200cfu/g

Casamino Acid; Light Tan; Homogenous; Free Flowing Powder; 1kg; (6%) 6 to 7 pH Casamino Acid; Light Tan; Homogenous; Free Flowing Powder; 1kg; (6%) 6 to 7 pH

Used as an alternate carbon source for wild-type yeast. Appearance: White, crystalline powder Solubility: Colorless, clear, complete after autoclaving Loss on Drying: 14-16% Optical Rotation (C=10, H2O): +105±2.0° Ash: ≤0.1% Glucose: ≤0.02% Microbiology: Total Bacteria: ≤1000cfu/g Yeast, Mold and Fungi: ≤100cfu/g Salmonella: Negative E. coli: Negative Stapylococcus: Negative Melting Point: 78-80°C Arsenic: ≤ 0.0001% Iron: ≤ 0.0005% Heavy Metals (Pb): ≤ 0.0005% Used as an alternate carbon source for wild-type yeast. Appearance: White, crystalline powder Solubility: Colorless, clear, complete after autoclaving Loss on Drying: 14-16% Optical Rotation (C=10, H2O): +105±2.0° Ash: ≤0.1% Glucose: ≤0.02% Microbiology: Total Bacteria: ≤1000cfu/g Yeast, Mold and Fungi: ≤100cfu/g Salmonella: Negative E. coli: Negative Stapylococcus: Negative Melting Point: 78-80°C Arsenic: ≤ 0.0001% Iron: ≤ 0.0005% Heavy Metals (Pb): ≤ 0.0005%

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 100U/mg

NC0764714 RAFFINOSE PENT LOW GLUC 2.5KG

Glycine is used in Tris-Glycine electrophoresis buffer formulations. This is a highly purified grade suitable for use in peptide synthesis. Special grade of Glycine used specifically for cell culture and Molecular Biology applications. Solubility: Complete solubility in water; slight solubility in alcohol and ether. pH (1%): 5.9-6.5 Residue on Ignition: ≤ 0.1% Heavy Metals (Pb): ≤ 0.002% Chloride: ≤ 0.007% Sulfate: ≤ 0.0065% Identity (IR): Conforms to reference USP Loss on Drying (Dry at 105°C for 2 hrs.): ≤ 0.2% RNase: None Detected DNase: None Detected Protease: None Detected Storage and Stability: Glycine is stable at RT. Powder may be stored at 4°C for long term storage. Stable for 12 months after receipt at 4°C. Glycine is used in Tris-Glycine electrophoresis buffer formulations. This is a highly purified grade suitable for use in peptide synthesis. Special grade of Glycine used specifically for cell culture and Molecular Biology applications. Solubility: Complete solubility in water; slight solubility in alcohol and ether. pH (1%): 5.9-6.5 Residue on Ignition: ≤ 0.1% Heavy Metals (Pb): ≤ 0.002% Chloride: ≤ 0.007% Sulfate: ≤ 0.0065% Identity (IR): Conforms to reference USP Loss on Drying (Dry at 105°C for 2 hrs.): ≤ 0.2% RNase: None Detected DNase: None Detected Protease: None Detected Storage and Stability: Glycine is stable at RT. Powder may be stored at 4°C for long term storage. Stable for 12 months after receipt at 4°C.

Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) �-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS- (1->4)-�-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc (1->4)-�-D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region. Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) �-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS- (1->4)-�-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc (1->4)-�-D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region.

Notch 1 is a transmembrane protein functioning in the development and determination of cell-fate (1). During maturation, the Notch molecule is cleaved by furin-like convertase at the extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the C-terminal Notch 1 fragment dissociates from its N-terminal counterpart and is further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus and activates Notch-related transcription. Applications: Suitable for use in Western Blot, Immunoprecipitation, and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4�C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4�C for short-term only. Notch 1 is a transmembrane protein functioning in the development and determination of cell-fate (1). During maturation, the Notch molecule is cleaved by furin-like convertase at the extracellular domain (2). Upon binding to a ligand such as Delta1, or upon extracellular calcium depletion, the C-terminal Notch 1 fragment dissociates from its N-terminal counterpart and is further cleaved between Gly1743 and Val1744 (3,4). The resulting activated cytosolic fragment translocates to the nucleus and activates Notch-related transcription. Applications: Suitable for use in Western Blot, Immunoprecipitation, and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4�C with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4�C for short-term only.

NC1202490 CHICK EMBRYO EXTRACT 20 ML

NC1249150 DEL/BODY TO ATRIAL NATURET/DEL

Used as a carbohydrate source in cell culture and molecular biology. Appearance: White, crystalline powder Purity: ≥99.5% Identity: To pass USP Specific Rotation: +52.6° to +53.2° Loss on Drying: ≤0.2% Residue on Ignition: ≤0.1% Acidity, ml: To pass USP Starch: To pass USP Dextrin: To pass USP Chloride: ≤0.018% Sulfate: ≤0.025% Arsenic: ≤0.0001% Heavy Metals (Pb): ≤ 0.0005% Meets or exceeds USP specifications Certificate of Origin: USA Used as a carbohydrate source in cell culture and molecular biology. Appearance: White, crystalline powder Purity: ≥99.5% Identity: To pass USP Specific Rotation: +52.6° to +53.2° Loss on Drying: ≤0.2% Residue on Ignition: ≤0.1% Acidity, ml: To pass USP Starch: To pass USP Dextrin: To pass USP Chloride: ≤0.018% Sulfate: ≤0.025% Arsenic: ≤0.0001% Heavy Metals (Pb): ≤ 0.0005% Meets or exceeds USP specifications Certificate of Origin: USA

Albumin, Bovine Serum, Fatty Acid Free, is our highest purity albumin specifically designed for use in all applications, including Immunology, Tissue Culture and Microbiology. Produced in the USA. Applications include Immunohematology, Serology, RIA, Blood Banking, ELISA Blocking and Protein Control formulation. Appearance: White to tan powder Albumin: 98% Fatty Acid: 0.05% IgG: None Detected pH: 6.7-7.3 Moisture: 5% Protease: None Detected Endotoxin: 3EU/mg Conductivity (10%): 3000uS Sterility: Filtered before lyophilization through a 0.2um membrane filter. Sodium (10%): As reported Chloride (10%): As reported Calcium (10%): As reported Iron (10%): As reported Vesicular Stomatitis Virus: Negative Bluetongue Virus: Negative Microbial Growth: 3CFU/ml Country of Origin: United States Certificate of Origin: All raw material used in the manufacturing of Bovine Serum Albumin, Fatty Acid Free is of United States origin and was manufactured in USDA-registered plants. Albumin, Bovine Serum, Fatty Acid Free, is our highest purity albumin specifically designed for use in all applications, including Immunology, Tissue Culture and Microbiology. Produced in the USA. Applications include Immunohematology, Serology, RIA, Blood Banking, ELISA Blocking and Protein Control formulation. Appearance: White to tan powder Albumin: 98% Fatty Acid: 0.05% IgG: None Detected pH: 6.7-7.3 Moisture: 5% Protease: None Detected Endotoxin: 3EU/mg Conductivity (10%): 3000uS Sterility: Filtered before lyophilization through a 0.2um membrane filter. Sodium (10%): As reported Chloride (10%): As reported Calcium (10%): As reported Iron (10%): As reported Vesicular Stomatitis Virus: Negative Bluetongue Virus: Negative Microbial Growth: 3CFU/ml Country of Origin: United States Certificate of Origin: All raw material used in the manufacturing of Bovine Serum Albumin, Fatty Acid Free is of United States origin and was manufactured in USDA-registered plants.

Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Yeast Nitrogen Base without amino acids (YNB w/o AA), Y2025, is used for selecting yeasts based on amino acid and carbohydrate requirements. In Y2025, Ammonium Sulfate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells. Compared to full YNB medium, Y2025 lacks Histidine, Methionine and Trypthophan. In combination with €œDrop Out € medium supplement mixtures, Y2025 provides an excellent medium to cultivate and select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins. Yeast Nitrogen Base (YNB) media have been prepared according the formulae of Wickerham (1943-51) and Burkholder (1943). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces cerevisiae. Yeast Nitrogen Base without amino acids (YNB w/o AA), Y2025, is used for selecting yeasts based on amino acid and carbohydrate requirements. In Y2025, Ammonium Sulfate is included as a readily available nitrogen source for nitrogen assimilation. The medium includes all other vitamins, mineral salts and trace elements required for a vigorous growth of yeast cells. Compared to full YNB medium, Y2025 lacks Histidine, Methionine and Trypthophan. In combination with €œDrop Out € medium supplement mixtures, Y2025 provides an excellent medium to cultivate and select auxothophic strains of yeast that requires the addition of essential nutrients like amino acids and/or vitamins.

RPMI-1640 was developed by Moore, et al., at Roswell Park Memorial Institute, hence the acronym RPMI.�The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.�RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes.�RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay. RPMI-1640 was developed by Moore, et al., at Roswell Park Memorial Institute, hence the acronym RPMI.�The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.�RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes.�RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

Peptone is an enzymatic digest of protein used to cultivate non-fastidious organisms, producing low-level growth. Deficient in carbohydrates, cystine and tryptophan. Appearance: Light tan, homogenous, free flowing powder pH: 7.0 �0.5 Amino Nitrogen: 6% Solubility (2%): Light yellow, clear, complete Total Nitrogen: 10% Reducing Sugars: Negative Undigested Protein: Negative Proteoses: Negative Nitrites: Negative Tryptophan: Negative Ash: 10% Moisture: 10% Typical Amino Acid Profile: Ala 8.5%, Arg 7.8%, Asp 6.2%, Cys 0.2%, Glu 9.8%, Gly 19.4%, His 0.8%, Ile 1.4%, Leu 2.9%, Lys 3.8%, Met 1.0%, Phe 1.9%, Pro 12.2%, Ser 3.7%, Thr 1.8%, Trp 0.05%, Tyr 0.7%, Val 2.2% Growth Supporting Properties (Agar/ Broth Formulations): Satisfactory Certificate of Origin: The raw animal products and enzymes, porcine tissue, used in the manufacturing of Peptone is derived from meat materials originating in the USA. During processing of the product, a batch is heated to a minimum of 80 �C. Peptone is an enzymatic digest of protein used to cultivate non-fastidious organisms, producing low-level growth. Deficient in carbohydrates, cystine and tryptophan. Appearance: Light tan, homogenous, free flowing powder pH: 7.0 �0.5 Amino Nitrogen: 6% Solubility (2%): Light yellow, clear, complete Total Nitrogen: 10% Reducing Sugars: Negative Undigested Protein: Negative Proteoses: Negative Nitrites: Negative Tryptophan: Negative Ash: 10% Moisture: 10% Typical Amino Acid Profile: Ala 8.5%, Arg 7.8%, Asp 6.2%, Cys 0.2%, Glu 9.8%, Gly 19.4%, His 0.8%, Ile 1.4%, Leu 2.9%, Lys 3.8%, Met 1.0%, Phe 1.9%, Pro 12.2%, Ser 3.7%, Thr 1.8%, Trp 0.05%, Tyr 0.7%, Val 2.2% Growth Supporting Properties (Agar/ Broth Formulations): Satisfactory Certificate of Origin: The raw animal products and enzymes, porcine tissue, used in the manufacturing of Peptone is derived from meat materials originating in the USA. During processing of the product, a batch is heated to a minimum of 80 �C.

NC1103677 DROPOUT MIX LYSINE

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.� Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME).�Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells.�MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.� Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.� Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal Medium (BME).�Subsequent studies using these and other cells in culture indicated that additions to BME could be made to aid growth of a wider variety of fastidious cells.�MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers.� Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.

A modified preparation of NGM for use in Nematode growth studies. Appearance: Light tan, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH (after autoclaving): 5.5-6.5 A modified preparation of NGM for use in Nematode growth studies. Appearance: Light tan, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH (after autoclaving): 5.5-6.5

NC9707806 PEPTONE 1 KG

Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Source: Arthrobactor luteus Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 20u/mg Zymolyase, produced by a submerged culture of Arthrobacter luteus (1), is an enzyme preparation which effectively lyses cell walls of viable yeast cells. An essential enzyme responsible for lysis of viable yeast cells in this preparation is b-1, 3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with b-1,3-linkages and releases laminaripentaose specifically as the main and minimum product unit (4,5,10,11). This lytic activity releases spheroplasts and protoplasts in the preparation of yeast DNA prior to restriction enzyme digestion and Southern Blot analysis. The extent of lysis of yeast cells by Zymolyase varies with yeast strain, growth stage of yeast and cultural condition (6-8). Further information related to Zymolyase is obtained in the references below. Source: Arthrobactor luteus Applications: Protoplast/Spheroplast Preparation Yeast Cell Fusion Yeast Cell Transformation Appearance: Lyophilized powder Activity: 20u/mg

Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells. Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

NC1081252 WILLIAMS MEDIA W/GLT 1L

Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder) Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)

A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58�C water bath. N1000 solution needs to completely cool to the desired temperature (58�C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58�C, place flask(s) onto stir plate(s). Maintain temperature at 58�C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution. A standard preparation for use in Nematode growth studies. Appearance: Light tan to pale yellow, homogeneous, free flowing powder Solubility without autoclaving: Light tan, incomplete Solubility with autoclaving: Light tan, clear, complete pH with autoclaving: As Reported Protocol: Cooling the N1000 solution after autoclaving: Place the sterile N1000 solution into a 58�C water bath. N1000 solution needs to completely cool to the desired temperature (58�C). If the medium is not properly cooled when the CaCl2, MgSO4 and K2HPO4 are added, crystals will form in the agar. Further preparing the medium: Once the N1000 solution has cooled to 58�C, place flask(s) onto stir plate(s). Maintain temperature at 58�C. Add the following: a) Sterile 1M Phosphate buffer*, pH 6.0: 25ml/liter N1000 solution b)Sterile 1M CaCl2: 1ml/liter N1000 solution c) Sterile 1M MgSO4: 1ml/liter N1000 solution.

NC0582690 LB BROTH 2.5KG

Tris Base Ultrapure (Encompass) Tris Base Ultrapure (Encompass)

LB Broth Lennox (Powder) 500g LB Broth Lennox (Powder) 500g

L-Broth (Luria Broth) is the standard nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, plasmid propagation, and protein expression. LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950s (1,2,3). �These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (4,5). �The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. �The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin. L-Broth (Luria Broth) is the standard nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, plasmid propagation, and protein expression. LB Broth media formulations have been industry standards for the cultivation of Escherichia coli since the 1950s (1,2,3). �These media have been widely used in molecular microbiology applications for the preparation of plasmid DNA and recombinant proteins (4,5). �The media are nutrient-rich formulations which provide peptides and peptones, vitamins, and trace elements. �The three formulations (Lennox, Miller and Luria) differ in the amount of sodium chloride, thus providing selection of the appropriate osmotic conditions for the particular bacterial strain and desired culture conditions. The low salt formulations, Lennox and Luria, are ideal for cultures requiring salt-sensitive antibiotics such as Zeocin.