Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This antibody can be used to monitor the expression of the human Liver/Bone/Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by Flow Cytometry.
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Synnuclein family of proteins is abundantly expressed in neuronal cytosol and presynaptic terminals. In vertebrates they are encoded by three different genes. Synucleins have been specifically implemented in three major diseases: Alzheimers (AD), Parkinsons (PD) and breast cancer. In AD, a peptide derived from alpha-synuclein forms an intrinsic component of plaque amyloid. In PD, an alpha-synuclein accumulates in Lewy bodies. An allele of alpha-synuclein has been linked to many familial cases of PD. In breast cancer increased expression of gamma synuclein correlates with the disease progression. Synucleins appear to be involved in the membrane plasticity in developing song control system of songbirds. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (frozen/ paraffin): 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 �C.
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Blue substrate used in the detection of b-galactosidase in bacteria or phage as a selection agent for cloning experiments utilizing the lacZ vector. Colonies expressing b-galactosidase will appear blue in the presence of XGAL. Others will appear white. Gene Cloning: In gene cloning, X-gal is used as a visual indication of whether a cell expresses a functional ²-galactosidase enzyme in a technique called blue/white screening. This method of screening is a convenient way of distinguishing a successful cloning product from other unsuccessful ones. The blue/white screening method relies on the principle of ±-complementation of the ²-galactosidase gene, where a fragment of the lacZ gene (lacZ±) in the plasmid can complement another mutant lacZ gene (lacZ”M15) in the cell. Both genes by themselves produce non-functional peptides, however, when expressed together, as when a plasmid containing lacZ± is transformed into a lacZ”M15 cells, they form a functional ²-galactosidase.
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Molecular Formula C37H66N2O12, Purity ≥95%, Molecular Weight 730.93, Melting Point >134ºC (dec.), Solubility: Chloroform (slightly), DMSO (Slightly) Methanol (slightly). Erythromycin A 9,11-Imino Ether is an impurity in the synthesis of Erythromycin, a macrolide antibiotic with broad spectrum of antibacterial activity.
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Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
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Drop-out Mix Complete (DOC) is used as a supplement to Drop-out Base for making synthetic, complete media formulations in accordance with Methods in Yeast Genetics. All lots are quality control tested using the appropriate yeast strains and transformants. Convenient Powders Useful for One-Hybrid and Two-Hybrid Systems Competitively Priced...! Dust-Free: Specially milled powder blends are designed to eliminate or greatly reduce airborne particulates Homogeneous: Proprietary blending technology ensures complete mixing of components Companion Products: D9500: Drop-out Base With Glucose (Powder) D9501: Drop-out Base With Raffinose (Powder) D9502: Drop-out Base With Galactose (Powder) D9510: Drop-out Base With Glucose and Agar (Powder) D9511: Drop-out Base With Raffinose and Agar (Powder) D9512: Drop-out Base With Galactose and Agar (Powder) Y2020: Yeast Nitrogen Base w/ AA & w/ AS, w/o Carbohydrate (YNB) (Powder)
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RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et. al. at Roswell Park Memorial Institute. Appearance: Pink, homogeneous, free flowing powder Solubility: Magenta, clear, complete pH: As reported Endotoxin: 1EU/ml
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Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity. Purity: ≥99.9% Appearance: White, crystalline powder pH (1M): 10.5-11.5 pKa: 8.0-8.4 Absorbance (260nm): ≤0.06 Absorbance (280nm): ≤0.05 Absorbance (290nm): ≤0.2 Solubility (1M): Colorless, clear, complete Loss on Drying: ≤0.2% Melting Point: 168-172°C Heavy Metals (Pb): ≤0.0001% Arsenic: ≤0.0001% Calcium: ≤0.0001% Copper: ≤0.0001% Iron: ≤0.0005% Magnesium: ≤0.0001% Insoluble Matter: ≤0.005% Residue on Ignition: ≤0.1% RNase: DNase: Protease: Meets specifications.
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Molecular Formula C₄H₁₁NO₃, Purity ≥99.9%, Molecular Biology Grade, Molecular Weight 121.14, Tris is one of the most widely used buffers in molecular biology and cell culture due to its low toxicity, stability and buffering capacity.
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