Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Boston Bioproducts Inc EDTA (0.5 M, pH 7.4, pH Adjusted with KOH) - 250ml
EDTA [Ethylenediaminetetraacetic acid] solution is commonly used in various molecular biology and protein biochemistry applications that require the chelation of divalent metal cations, such as calcium (Ca2+) and magnesium (Mg2+). EDTA is used extensively as an anticoagulant in blood samples as it chelates the calcium present, preventing coagulation and preserving blood cell morphology. It is also used in cell lysis buffers. This solution is adjusted with KOH.
Newcomer Supply Phosphate Buffer 0.1M, pH 7.2 to 7.4, 1 gallon
Use: IHC wash and rinse. Shelf Life is 1 year from date of manufacture. Store at 2-8°C.
MOLTOX Molecular Toxicology Inc 0.1M Tris-HCL Buffer, pH 8.0, 1000 mL/bottle, Store at Room Temperature
MOLTOX manufactures a wide variety of buffers for your laboratory needs. All MOLTOX reagents are accompanied by a GLP-compliant Formulation and Quality Control statement.
Boston Bioproducts Inc Paraformaldehyde (4%, PBS with Mg & EGTA) - 250ml
Paraformaldehyde (4% PBS with Mg and EGTA) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffered Saline (2X) - 500ml
HEPES Buffered Saline (2X) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Potassium Phosphate Buffer (0.2 M, pH 7.4) - 1L
Potassium Phosphate Buffer (0 2 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc 50 mM Sodium Phosphate, 2 mM EDTA (pH 7.0) - 2x1L
Bioprocess Grade - 50 mM Sodium Phosphate 2 mM EDTA (pH 7 0) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, for Western Blot Washing) - 1L
Phosphate Buffered Saline PBS (10X for Western Blot Washing) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Buffered Saline-Tween, TBST - 2x1L
Bioprocess Grade - Tris-Buffered Saline-Tween TBST - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.0) - 1L
Tris-HCl Buffer (1 M pH 8 0) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc TAPS Buffer (0.2 M, pH 8.5) - 100ml
TAPS Buffer (0 2 M pH 8 5) - 100ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Sodium Phosphate Buffer (1 M, pH 7.2) - 50ml
Sodium Phosphate Buffer (1 M pH 7 2) - 50ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HBS-EP Buffer (1X, pH 7.4) - 4x500ml
HBS-EP Buffer (1X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, RNase Free) - 250ml
Phosphate Buffered Saline PBS (10X RNase Free) - 250ml (For Research Use and Further Manufacturing Only)