Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Selleck Chemical LLC Trometamol S4176-50mg
Trometamol(Tromethamine) is a proton acceptor used to treat acidemia
Boston Bioproducts Inc 10mM Tris-HCl pH 8 1L
Tris-HCl Buffer (10 mM pH 8 0) - 1L (For Research Use and Further Manufacturing Only)
Medchemexpress LLC Nvp-tae 226 | 761437-28-9 | MFCD12031516 | 99.8% | C23H25ClN6O3 | 10MG
NVP-TAE 226 is a potent, ATP-competitive small-molecule inhibitor of focal adhesion kinase (FAK) and insulin-like growth factor 1 receptor (IGF-1R), used in preclinical oncology and signal-transduction research. It exhibits activity across multiple kinases and has demonstrated antitumor effects in vitro and in vivo.
- Potent FAK inhibition (IC50 5.5 nM).
- Dual activity against IGF-1R and Pyk2 (IC50 140 nM and 3.5 nM).
- Additional activity against InsR, c-Met, KDR, and Flt3.
- High purity suitable for research (~99.8%).
- Stable as powder under recommended storage conditions.
Seracare Life Sciences Inc 10X TRIS-GLYCINE-SDS BUFFER
Tris-Glycine-SDS (TGS) is a running buffer for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. pH=8.3. Conductivity controlled.
Thermo Scientific Production Chemicals and Services TRIS HCL GMP GRADE 10KG
NC3654762 TRIS HCL GMP GRADE 10KG
Teknova 1M Tris-HCl, pH 9.0 Solution, 20L Bag, Sterile. 4 Pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Kd Medical Inc TBE (TRIS-BORATE EDTA), 10X
Tris Borate EDTA is used for polyacrylamide and agarose gel electrophoresis. 10X TBE is concentrated and ready to be diluted to a 1X working solution. One 500ml bottle.
eMolecules 199003-22-0 | tert-butyl 4-(hydroxymethyl)pyrazole-1-carboxylate | Pharmablock | MFCD24335255 | 198.222 | C9H14N2O3 | 97.000 | CC(C)(C)OC(=O)n1cc(CO)cn1 | 1g | 551300363
tert-butyl 4-(hydroxymethyl)pyrazole-1-carboxylate | Pharmablock | 199003-22-0 | MFCD24335255 | 198.222 | C9H14N2O3 | 97.000 | CC(C)(C)OC(=O)n1cc(CO)cn1 | 1g | 551300363