Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bio Rad Laboratories XT MOPS RUNNING BUFFER 500ML
Extended range protein standard, 500ul, 50 applications. Product contains 12 bands from 2kD to 250kD. 250, 150, 100, 50, 37, 20, 15, 10 & 5 kD are blue, 75, 25 and 2kD are pink.
Sigma Aldrich Fine Chemicals Biosciences Trizma hydrochloride, 1185-53-1, MFCD00012590, 500 g
Linear Formula: NH2C(CH2OH)3 · HCl, Molecular Weight: 157.60, BioPerformance Certified, cell culture tested, ≥99.0% (titration), Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride.
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) Pre-set crystals BioPerformance Certified, pH 7.0, average Mw 154.8 | 100G
Trizma(R) Pre-set crystals BioPerformance Certified, pH 7.0, average Mw 154.8 | 100G
Rockland Immunochemicals 10X PBST (0.2 M Potassium Phosphate 1.5 M Sodium Chloride, 0.5% (v/v) Tween-20, pH 7.2) - MB-075-1000
Phosphate Buffer Saline-Tween is commonly use in washing procedure while performing western-blot, ELISA and, more generally immuno-assays.
Santa Cruz Biotechnology Tris Buffered Saline with Tween 20: 5 L of 20X, 5 L
Tris Buffered Saline with Tween 20: 5 L of 20X is a high-quality 20X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X, for Western Blot Washing) - 4L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Boca Scientific Inc Antibody Stabilizer PBS Base, 125ml
Antibody Stabilizer conserves the structure of proteins and antibodies to prevent loss of functionality due to storage. With refrigerated storage, Antibody Stabilizer prevents the need for freezing and thawing. Both TRIS-based and PBS-based Antibody Stabilizers are available, allowing the user to select the optimal buffer base for the assay.
STEMCELL Technologies HBSS with 10 mM HEPES, Without Phenol Red, Size: 500 mL
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating, or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions, and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
Teknova 1M Tris-HCl, pH 8.0. 1000mL, Sterile, Endotoxin Tested, PCR Grade.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Boston Bioproducts Inc Tris-Base (2 M, pH > 10.0) - 250ml
Tris-base is a widely used biological buffer in various research applications in biochemistry, molecular biology, and cell biology. The buffering capabilities of Tris-base are effective in the pH range of 7.0-9.0, making it suited for various biological applications. This buffer is widely used in protocols for DNA and RNA extraction, in situ hybridization, protein extraction from cells, in agarose gel electrophoresis, polyacrylamide gel electrophoresis and as a wash buffer in Western blotting.
Genscript Corporation Tris-MOPS-SDS Running Buffer Powder
Tris-MOPS-SDS Running Buffer Powder are used for ExpressPLUS and SurePAGE gel transfer. Using proprietary techniques, Tris-MOPS-SDS Running Buffer Powder are made to have long shelf life, high resolution, fast electrophoresis, smaller volume.