Trifluoroacetic Acid
Trifluoroacetic acid is a corrosive organofluorine compound that is structurally analogous to, but stronger than, acetic acid. Available in various quantities and reagent grades, it is used in NMR spectroscopy, mass spectrometry, organic synthesis, etc.
Almost 100,000-fold more acidic than acetic acid, TFA is widely used in organic chemistry. TFA is used as a reagent in organic synthesis because of its properties: volatility, organic solvent solubility, and acidic strength. It is less oxidizing than sulfuric acid and is more readily available in its anhydrous form than some other acids . Other features include:
- Used in acid-catalyzed reactions, especially peptide synthesis (to cleave esters)
- Dissolves protein when mixed with liquid SO2
- Removes t-butyl derived side-chain protecting groups in Fmoc peptide synthesis
- Can remove the t-butoxycarbonyl protecting group in organic synthesis
- At low concentrations, acts as an ion-pairing agent for peptides and small proteins in organic compound liquid chromatography
- For acid-stable materials, can be a solvent for NMR spectroscopy
- Acts as a calibrant in mass spectrometry
- Used to produce trifluoroacetate salts
- An ingredient in adhesives, sealants, paints, and coatings
When TFA combines with bases and metals, especially light metals, a strong exothermic reaction occurs. When mixed with lithium aluminum hydride (LAH), the reaction is explosive.
Although nonflammable, TFA is corrosive to skin, eyes, and mucous membranes and requires careful use and handling. It is harmful when inhaled, causes severe skin burns and eye damage, and is toxic to aquatic organisms at even low concentrations.
TFA is also a product of the metabolic breakdown of the anesthetic agent halothane. It is the suspected cause of halothane-induced hepatitis.
![Medchemexpress LLC 6-Benzothiazolamine, N-[6-[2-[(cyclohexylmethyl)amino]-4-(trifluoromethyl)phenyl]-4-pyrimidinyl]- (TFA) | 99.1% | 597.58 | 25 MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000438226-Logo.png-250.jpg){kind=logo}
Medchemexpress LLC 6-Benzothiazolamine, N-[6-[2-[(cyclohexylmethyl)amino]-4-(trifluoromethyl)phenyl]-4-pyrimidinyl]- (TFA) | 99.1% | 597.58 | 25 MG
AMG 7905 TFA is a hypothermia-inducing transient receptor potential vanilloid type 1 (TRPV1) antagonist. It potentiates TRPV1 channels activation by protons and drives the reflectory inhibition of thermogenesis and tail-skin vasoconstriction, while potently blocking channel activation by capsaicin.
- Potentiates TRPV1 channels activation by protons
- Drives reflectory inhibition of thermogenesis and tail-skin vasoconstriction
- Potently blocks channel activation by capsaicin
Medchemexpress LLC AUNP-12 (TFA) | 75.64% | 10 MG
AUNP-12 (TFA) is a peptide compound primarily used for research purposes. This product, identified by batch number 44290, features a complex molecular structure.
- Molecular formula: C142H226N40O48.C2HF3O2
- Actual peptide content: 75.64%
- Theoretical peptide content: ≥60.0%
Medchemexpress LLC Peptide5 TFA | 98.9% | 1509.60 | 1 MG
A connexin 43 mimetic peptide, it reduces animal swelling, astrogliosis, and neuronal cell death after spinal cord injury. It also inhibits NLRP3 inflammasome, and is an anti-inflammatory agent.
- Reduces animal swelling after spinal cord injury.
- Reduces astrogliosis after spinal cord injury.
- Reduces neuronal cell death after spinal cord injury.
- Inhibits NLRP3 inflammasome.
- Acts as an anti-inflammatory agent.
Medchemexpress LLC TFLLR-NH2(TFA) | 1313730-19-6 | 99.6% | 761.83 | 25 MG
TFLLR-NH2 (TFA) is a selective PAR1 agonist with an EC50 of 1.9 μM. It stimulates concentration-dependent increases in [Ca2+]i, upregulates E-cadherin, and downregulates vimentin expression in SW620 cells. It also induces a dose-dependent increase of secreted TGF-β1 in activated platelets. In vivo, it causes marked and sustained oedema and Evans blue extravasation, and produces contraction and relaxation in rat duodenal smooth muscle.
- Selective PAR1 agonist with an EC50 of 1.9 μM
- Appears as a white to off-white solid
- Soluble in DMSO and H2O at 100 mg/mL
- Upregulates E-cadherin and downregulates vimentin expression
- Induces dose-dependent increase of secreted TGF-β1
- Stimulates oedema and Evans blue extravasation in vivo
- Produces contraction and relaxation in rat duodenal smooth muscle
Medchemexpress LLC D2A21 TFA | 95.6% | 2775.42 | 5 MG
D2A21 TFA is an antimicrobial peptide with significant antimicrobial activity against Pseudomonas and a bactericidal effect on burn scabs. It also exhibits antitumor activity and can be used in prostate cancer research.
- Antimicrobial peptide with significant antimicrobial activity against Pseudomonas
- Bactericidal effect on burn scabs
- Has antitumor activity
- Can be used in prostate cancer research
![Medchemexpress LLC Benzamide, N-(3S)-1-azabicyclo[2.2.2]oct-3-yl-2,5-dimethoxy-, 2,2,2-trifluoroacetate | 1336913-03-1 | 99.9% | 404.38 | 25 MG](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000438811-Logo.png-250.jpg){kind=logo}
Medchemexpress LLC Benzamide, N-(3S)-1-azabicyclo[2.2.2]oct-3-yl-2,5-dimethoxy-, 2,2,2-trifluoroacetate | 1336913-03-1 | 99.9% | 404.38 | 25 MG
PSEM 89S TFA is a selective and brain penetrant agonist for ion channels, orthogonally selective for Q79G and L141F. It is intended for research use only.
- Activates layer 2/3 cortical neurons expressing PSAML141F,Y115F-5HT3 high conductance.
- Reversibly silences transfected neurons by reducing cellular input resistance.
- Reduces photostimulation-evoked feeding in mice with PSAML141F,Y115F-glycine receptor.
- Demonstrates good brain penetrance in mice after intraperitoneal administration.
- Suppresses fos in ChR2-expressing neurons in Agrp-cre mice.
- Shows rapid rise in serum and brain, cleared within 1 hour in C57BL/6 mice.
Medchemexpress LLC Balixafortide TFA (POL6326 TFA) | 1051366-32-5 | 99.9% | 1978.16 | 25 MG
Balixafortide TFA (POL6326 TFA) is a potent, selective, and well-tolerated peptidic CXCR4 antagonist with an IC50 less than 10 nM. It demonstrates significant selectivity for CXCR4, approximately 1000-fold, over various other receptors including CXCR7. This compound effectively blocks β-arrestin recruitment and calcium flux with IC50s less than 10 nM, functioning as a potent hematopoietic stem and progenitor cell (HSPC) mobilizing agent with observed anti-cancer effects.
- Potent and selective CXCR4 antagonist
- Demonstrates high selectivity over other receptors like CXCR7
- Blocks β-arrestin recruitment and calcium flux
- Functions as a potent hematopoietic stem and progenitor cell (HSPC) mobilizing agent
- Exhibits anti-cancer effects
- Optimized for favorable mouse ADME properties
Medchemexpress LLC W146 trifluoroacetate | 909725-62-8 | 98.2% | 456.39 g/mol | C18H28F3N2O6P | 25 MG
W146 trifluoroacetate is a research-grade selective antagonist of sphingosine-1-phosphate receptor 1 (S1PR1). Supplied as the trifluoroacetate salt in a white to off-white solid form, it has an EC50 of 398 nM, a molecular weight of 456.39 g/mol, and is typically supplied at high purity for in vitro pharmacology and receptor signaling studies. Not for human or clinical use.
- Selective S1PR1 antagonist with EC50 398 nM
- Provided as trifluoroacetate salt for stability
- High purity suitable for biochemical assays
- White to off-white solid for easy handling
- Intended for in vitro pharmacology and receptor signaling studies
Medchemexpress LLC Colivelin TFA | 2803948-60-7 | 99.5% | 2759.12 | 25 MG
Colivelin TFA is a brain-penetrant neuroprotective peptide that acts as a potent activator of STAT3, suppressing neuronal death in vitro. It demonstrates long-term beneficial effects against neurotoxicity, amyloid-beta (Aβ) deposition, neuronal apoptosis, and deficits in synaptic plasticity associated with neurodegenerative diseases. This compound holds potential for the treatment of Alzheimer's disease and ischemic brain injury.
- Neuroprotective properties
- Potent activator of STAT3
- Suppresses neuronal death
- Exhibits long-term beneficial effects against neurotoxicity
- Reduces amyloid-beta (Aβ) deposition
- Prevents neuronal apoptosis
- Addresses synaptic plasticity deficits
- Potential treatment for Alzheimer's disease
- Potential treatment for ischemic brain injury
Medchemexpress LLC Hexa-His TFA | 1456728-20-3 | 99.8% | 954.87 | 25 MG
Hexa-His TFA is a peptide consisting of 6 His residues, used as a metal binding site for the recombinant protein.
- Consists of 6 His residues
- Used as a metal binding site
- Suitable for recombinant protein applications
- Molecular weight of 954.87
- Purity of 99.8%
- Appearance is solid, white to off-white
- Recommended storage for powder: -80°C for 2 years or -20°C for 1 year
- Recommended storage in solvent: -80°C for 6 months or -20°C for 1 month
- Store sealed and away from moisture
Medchemexpress LLC Gln-AMS TFA | 99.9% | 588.47 | 100 MG
Gln-AMS (TFA) is a type Ia aminoacyl-tRNA synthetase (AARS) inhibitor, specifically inhibiting glutaminyl-tRNA synthetase (GlnRS) with a Ki of 1.32 μM.
- Inhibits glutaminyl-tRNA synthetase (GlnRS)
- Type Ia aminoacyl-tRNA synthetase (AARS) inhibitor
- White to off-white solid
- Purity of 99.85%
- Soluble in DMSO at 100 mg/mL
- Store at -20°C under nitrogen
Medchemexpress LLC (RXR)4XB TFA | 96.6% | 5 MG
(RXR)4XB TFA is a cell-penetrating peptide that binds phosphorodiamidate morpholino oligomers (PMOs), forming peptide-conjugated PMOs (PPMOs). It improves the delivery of PMO into bacterial cells.
- Prevents biofilm formation
- Inhibits *Pseudomonas aeruginosa* with an MIC50 of 0.5 to 16 μM
- Reduces bacterial burden in mouse acute pneumonia models
Medchemexpress LLC SJ1008030 TFA | 2863634-97-1 | 99.8% | 987.96 | 1 MG
SJ1008030 TFA is a JAK2 PROTAC designed for selective degradation of JAK2. This compound inhibits MHH-CALL-4 cell growth and is suitable for leukemia research. It degrades JAKs, GSPT1, and IKZF1 in a dose-dependent manner, indicating inhibition of the JAK-STAT signaling pathway.
- Selectively degrades JAK2
- Inhibits MHH-CALL-4 cell growth with an IC50 of 5.4 nM
- Can be used for leukemia research
- Degrades JAKs, GSPT1, and IKZF1 in a dose-dependent manner
- Indicates inhibition of the JAK-STAT signaling pathway
Medchemexpress LLC Nls-Stax-h (Tfa) | 2872559-21-0 | 98.9% | 3559.28 | 100 UG
Nls-Stax-h is a selective, cell-permeable, stapled peptide that acts as a Wnt signaling inhibitor. It has an Ic50 of 1.4 μM and effectively inhibits β-catenin-transcription factor interactions. This compound demonstrates anti-proliferative effects on cancer cells.
- Selective Wnt signaling inhibitor.
- Cell-permeable.
- Stapled peptide.
- Inhibits β-catenin-transcription factor interactions.
- Shows anti-proliferation of cancer cells.
- Inhibits proliferation of colorectal cancer cells at 10 μM over 72 h, reducing viability by more than 80% in SW-480 and DLD-1 cells.
- Inhibits migration of colorectal cancer cells at 5 and 10 μM over 24 h, resulting in dose-dependent inhibition of wound closure (52% at 5 μM, and 24% at 10 μM in DLD-1 cells).