Protein Method Blocking Buffers
Electron Microscopy Sciences Aurion R-Gent SE-EM Kit 30 ML
AURION R-Gent SE-EM is a high efficiency silver enhancement reagent for electron microscopy. It intensifies the gold particles by homogeneous deposition of metallic silver on the particle surface. The resulting electron dense signal is easily detected and is compatible with heavy metal counterstaining. The reagent has extremely delayed auto-nucleation and can be used under standard laboratory light conditions. It also has low viscosity, which is especially advantageous for pre-embedding immunogold applications.
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting 3-PACK (3 x 500 ml) - MB-070-003
Western Blot Blocking Solution is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Biotang Inc BSA- Blocking Buffer in TBS and Tween-20, 250 ml
BSA- Blocking Buffer in TBS and Tween-20, 250 ml.
Electron Microscopy Sciences Aurion Tween-20 10 ML
Tween-20 is a non-ionic detergent with a molecular weight of about 600 and a critical micelle concentration (CMC) of 0.06-0.07% in water at room temperature. Its working mechanism may in part be based on its action as a detergent, binding to the hydrophobic moieties of water insoluble compounds, rendering them hydrophilic. In addition, immuno-compounds may become incorporated into micelles when the Tween-20 concentration is higher than the CMC, for instance at 0.1 % in PBS at pH 7.4.
Biotang Inc BLOTTO TWEEN IN PBS 250 ML, IBB-151, 5% nonfat dry milk with 0.05% Tween-20 in PBS (10 mM Sodium phosphate, 2 mM potassium phosphate,10 mM KCl,137 mM NaCl), 7.4 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
BLOTTO TWEEN IN PBS 250 ML, IBB-151, 5% nonfat dry milk with 0.05% Tween-20 in PBS (10 mM Sodium phosphate, 2 mM potassium phosphate,10 mM KCl,137 mM NaCl), 7.4 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
LICOR Intercept T20 (PBS) Protein-Free Antibody Diluent (500mL)
Intercept T20 (PBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (PBS) Protein-Free Blocking Buffer preformulated with Tween 20 for use in a phosphate-buffered saline (PBS) system. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (PBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
LICOR Intercept T20 Protein-Free Antibody Diluent (3x500mL)
Intercept T20 (TBS) Protein-Free Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Protein-Free Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. This diluent can be used where animal-based products are prohibited. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Protein-Free Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
LICOR Intercept (TBS) Protein-Free Blocking Buffer (10x500mL)
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Biotium Napsin A (Lung Adenocarcinoma Marker)(NAPSA/1865R), CF740 conjugate, 0.1mg/mL
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs. Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other labels. CF Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF405S and CF405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Enzo Life Sciences Antibody Blocker/Diluent (100ml)
The Antibody Blocker/Diluent can be used to block nonspecific staining due to antibodies during immunostaining. It can also be used as a diluent for the POLYVIEW PLUS detection reagents or as a primary antibody diluent. This product is intended for immunohistochemistry and in situ hybridization with formalin-fixed parafiin-embedded tissue sections and frozen sections. The blocker/diluent may also be used with blood smears, cytosmears, and cell preparations. Long Term Storage: +4°C. Applications: IHC ISH (in situ hybridization).
Research Products International Corp TBS with Tween, 20X, 7.4 pH, Sterile Buffered, 1 Liter
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is a commonly used buffer solution in the life science marketplace. TBS stands for Tris-Buffered Saline, and Tween refers to Tween 20, a nonionic detergent.
TBS with Tween is primarily used in various applications such as Western blotting, immunohistochemistry, and antibody-based assays. It serves as a buffer for sample dilution, antibody washing, and blocking steps during these experimental procedures.
The buffer solution is prepared at a 20X concentration, meaning it is twenty times more concentrated than the final desired concentration. This concentrated form allows for efficient storage and transportation.
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is specifically formulated to have a pH of 7.4, which is the physiological pH of many biological systems.
LICOR TBS Blocking Buffer and PVDF Kits (single buffer kit)
The non-mammalian Intercept Blocker in these kits is in TBS (Tris-buffered saline) containing 0.06% Kathon II. The Immobilon-FL PVDF membrane included in these kits has been tested to ensure a low background for infrared Western blot success. This kit contains Intercept (TBS) Blocking Buffer (500 mL) and Millipore Immobilon-FL PVDF Membrane (1 roll; 0.45µm, 26.5 cm x 3.75 m).