Protein Method Blocking Buffers
Boston Bioproducts Inc Phosphate Buffered Saline-Tween, PBST (10X, with 1% Tween-20, pH 7.4) - 1L
10X Phosphate Buffered Saline with Tween-20 (PBST) is a specialized variant of PBS, supplemented with Tween-20, a non-ionic surfactant. Tween-20 minimizes the non-specific binding and background noise, resuting in enhanced signal to noise ratio. PBST is often used in immunoassays such as ELISA and Western Blotting, where it serves as a washing and blocking buffer. With a stable buffering pH range between 7.0 and 7.4, PBST maintains ionic strength and protein solubility for biological assays.
LAB BIOREAGENTS Strongblock I 500ML
Lab Bioreagents StrongBlocks (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlocks (I, II, III) are a variety of buffers designed by different blocker components. During the assay, the test different blocking buffers helps to develop a immunoassay with low background noise and high signal-to-noise.
SCANTIBODIES LABORATORY INC HBR PLUS (3KC545) 1.0G
The 3KC545 Heterophilic Blocking Reagent Plus from Scantibodies Laboratory, Inc. was developed as an alternative for HBR-1. This product is compounded with immunoglobulins with different characteristics. Therefore, in addition to its active blocking characteristics, the special formulation and production procedures enhance its efficacy in its passive blocking ability as well. It has a concentration of 10.0 +/- 1.0 mg/ml.
Quality Biological Inc DPBS with TWEEN 20 without Ca & Mg, 10 Liters
DPBS can be used to provide a buffer system to maintain cell culture media in the physiological pH range of 7.0 to 7.6. ¿ DPBS serves as an irrigating, transporting or diluting fluid for mammalian cells, while maintaining cell tonicity and viability for a limited period of time. ¿ DPBS is also used as a diluent of fresh red blood cells to study the trapping of red blood cell aggregate, to wash the wells of microtiter plates, and to wash cells during preparation and serial transfer. ¿ 1X working solution is recommended ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Biocare Medical, LLC intelliPATH FLX Peroxidase Blocking Reagent - 20 ml
Peroxidazed Blocking Reagent is a highly stable form of hydrogen peroxide for blocking endogenous peroxidase. It is very effective for blocking non-specific staining in red blood cells. It is non-flammable, safer and less toxic when compared to hydrogen peroxide/methanol formulations.
Bioworld BBS/Fish Gelatin (10X), 200 mL
BBS/Fish Gelatin 10XBBS Buffered fish gelatin 5% blocks (10X), diluents and coating solutions are supplied as 10X concentrates. Technical Information: pH range: 8.0 (+/- 0.1) formulated in a borate buffered physiologic saline bufferSold as 2 x 100mL or 500mL.
Research Products International Corp Casein Blocking Solution in TBS, 1 Liter
Contains 3% Casein in TBS buffer. Optimized for use in western blotting to minimize non-specific binding of antibodies.
SCANTIBODIES LABORATORY INC HBR 1, PURIFIED (3KC533) 1.0G
The 3KC533 Heterophilic Blocking Reagent 1, Purified from Scantibodies Laboratory, Inc. contains specific murine immunoglobulins that block the heterophilic interaction by active binding to the heterophilic antibodies, which are capable of cross-linking the capture and the detection antibodies used in the immunoassay, resulting in false positive readings. The attachment of HBR-1 to the heterophilic antibodies blocks this cross-linking, and eliminates the interference caused by the heterophilic antibodies in the humoral fluids. In addition to its active blocking, this product is also characteristic of its passive blockage of the heterophilic interaction as well. The HBR is a liquid reagent with a protein concentration of 20 ± 2 mg/ml. The immunoglobulins are dissolved in a phosphate buffer with a pH of 7.2-7.6. The immunoglobulins in this product are at a purity of greater than or equal to 95%.
Enzo Life Sciences Antibody Blocker/Diluent (100ml)
The Antibody Blocker/Diluent can be used to block nonspecific staining due to antibodies during immunostaining. It can also be used as a diluent for the POLYVIEW PLUS detection reagents or as a primary antibody diluent. This product is intended for immunohistochemistry and in situ hybridization with formalin-fixed parafiin-embedded tissue sections and frozen sections. The blocker/diluent may also be used with blood smears, cytosmears, and cell preparations. Long Term Storage: +4°C. Applications: IHC ISH (in situ hybridization).
Bioworld Casein-Blocking Buffer in PBS, 1 L
Product DescriptionCasein-blocking buffer in PBS is a purified casein protein solution used for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods. Phosphate-buffered saline formulations are used for blocking excess binding sites on membranes and microplates in antibody-based detection methods.This blocking buffer was purified from milk and contains 1% (w/v) casein that corresponds to the optimal concentration for most applications.
Crescent Chemical Co Inc Denhardt's Solution, 50X Concentration, 10mL
Denhardt's Solution, 50X Concentration, 10mL, Molecular Biology Grade, DNase/RNase not detected.Suitable for nucleic acid hybridization. Denhardt's solution is a mixture of blocking agents capable of saturating non-specific binding sites and to be used in membrane-based hybridization protocols. It is recommended for use with nylon membranes. Storage Conditions: -15 °C TO -25 °C
Research Products International Corp TBS with Tween, 20X, 7.4 pH, Sterile Buffered, 1 Liter
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is a commonly used buffer solution in the life science marketplace. TBS stands for Tris-Buffered Saline, and Tween refers to Tween 20, a nonionic detergent.
TBS with Tween is primarily used in various applications such as Western blotting, immunohistochemistry, and antibody-based assays. It serves as a buffer for sample dilution, antibody washing, and blocking steps during these experimental procedures.
The buffer solution is prepared at a 20X concentration, meaning it is twenty times more concentrated than the final desired concentration. This concentrated form allows for efficient storage and transportation.
TBS with Tween, 20X, 7.4 pH, Sterile Buffered is specifically formulated to have a pH of 7.4, which is the physiological pH of many biological systems.
SCANTIBODIES LABORATORY INC NON-SPECIFIC ANTIBODY BLOCKING TUBE (31X761) 50PK
The 31X761 NABT (Non-Specific Antibody Blocking Tube) from Scantibodies Laboratory, Inc. contains immunoglobulins which the non-specific antibodies in the serum or plasma samples bind to, thus causing them to be blocked from interfering in antibody detection immunoassays. The NABT also allows for the rapid and simple elimination of false positive non-specific antibody interference in plasma or serum for detection assays (i.e., anti-HCV, HIV, Toxoplasmosis, Rubella, CMV, Herpes, Tg, Thyroglobulin, TPO Thrombopoietin, etc.). The reagent is in the form of a lyophilized pellet at the bottom of the tube and each tube contains enough reagents to inactivate the non-specific antibodies in 500 μl of sample material.