Protein Method Blocking Buffers
LI COR BIOTECH LLC Intercept (TBS) Protein-Free Blocking Buffer (3x500mL)
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
LI COR BIOTECH LLC Intercept (PBS) Protein-Free Blocking Buffer (10x500mL)
Intercept (PBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. This buffer contains no proteins, for assays where use of animal-based products is prohibited. They are an excellent choice for biotin-streptavidin detection and suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
LI COR BIOTECH LLC Intercept (TBS) Protein-Free Blocking Buffer (10x500mL)
Intercept (TBS) Protein-Free Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays where animal-based products are prohibited. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS). It contains no proteins, for assays where animal-based products are prohibited, it can be used with the widest range of targets for Western blot detection, it is ideal for primary antibodies that are optimized for Tris-based buffer systems, it can be used for detection of phosphorylated targets, it has superior performance for alkaline phosphatase detection, and it is an excellent choice for biotin-streptavidin detection. Intercept (TBS) Protein-Free Blocking Buffer is a ready-to-use blocker formulation in TBS and is stored at 4 °C. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection.
Boston Bioproducts Inc Phosphate Buffered Saline-Tween, PBST (10X, with 1% Tween-20, pH 7.4) - 1L
10X Phosphate Buffered Saline with Tween-20 (PBST) is a specialized variant of PBS, supplemented with Tween-20, a non-ionic surfactant. Tween-20 minimizes the non-specific binding and background noise, resuting in enhanced signal to noise ratio. PBST is often used in immunoassays such as ELISA and Western Blotting, where it serves as a washing and blocking buffer. With a stable buffering pH range between 7.0 and 7.4, PBST maintains ionic strength and protein solubility for biological assays.
Teknova 1X PBS Solution with 0.05% Tween-20 (pH 7.4). 20L Carboy, Sterile
137mM Sodium Chloride, 2.7mM Potassium Chloride,4.3mM Sodium Phosphate, 1.4mM Potassium Phosphate,0.05% Tween (v/v)
RIGAKU REAGENTS Block, Wizard pH Buffer, 96 formulations, 1mL volumes
The Wizard pH Buffer screen is an optimization screen that can be applied to your crystallization hit optimization to simultaneously explore the effects of pH and buffer composition on crystal growth. The Wizard pH Buffer screen is a set of 96 unique buffer solutions encompassing 12 unique buffer systems at 8 pH points, incrementally varied by 0.4 pH units.96-well block plate formulations are 1.0 ml each in a 96 deep-well matrix block plate. All formulations are prepared with ASTM type-1 water and high-purity chemicals, 0.2 �m filtered in sterile packaging.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (20X, with 1% Tween-20, pH 7.4) - 1L
20X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Biocare Medical, LLC intelliPATH FLX Peroxidase Blocking Reagent - 20 ml
Peroxidazed Blocking Reagent is a highly stable form of hydrogen peroxide for blocking endogenous peroxidase. It is very effective for blocking non-specific staining in red blood cells. It is non-flammable, safer and less toxic when compared to hydrogen peroxide/methanol formulations.
Genetex Inc Trident Universal Protein Blocking Reagent (animal serum free)
The Trident Universal Protein Blocking Reagent (animal serum free) is in a ready to use format. Western blot: Immerse the filter in a sufficient amount of Blocking Reagent and incubate for 30 minutes at RT with gentle shaking. IHC/ICC: Incubate tissue sections/ cell smears in Blocking Reagent for 10 minutes at RT or 37ºC. Note: Optimal incubation times should be determined by the researcher.
Santa Cruz Biotechnology Tris Buffered Saline with Tween 20: 1 L of 20X, 1 L
Tris Buffered Saline with Tween 20: 1 L of 20X is a high-quality 20X concentrate for dilution to a 1X working solution commonly used as a wash solution for western blot membranes and microtiter plate wells in ELISA assays. Tris Buffered Saline with Tween 20 (TBST) is an optimal formulation of pH stabilizers, salts and detergents designed to effectively remove excess material from the microtiter plate wells without disrupting the ELISA binding reaction. By maintaining the proper buffering environment, unbound components can be washed away without suppressing antigen-antibody binding interactions, thereby reducing nonspecific background and increasing the specific signal.
Leica Biosystems PEROXIDASE BLOCK NOVOCASTRA
Novocastra Peroxidase Block, RE7101, is intended for use in the peroxidase based immunohistochemical (IHC) staining procedures. The presence of pseudoperoxidase (erythrocytes) and endogenous peroxidase in paraffin sections to be stained by immunoperoxidase procedures, can result in nonspecific staining. A method for the blocking of pseudoperoxidase was described (StreefkerkJG, Journal of Histochemistry and Cytochemistry. 20: 829 (1972)). This product is used in a peroxidase based IHC procedure. Incubating sections with Novocastra Peroxidase Block, RE7101, can neutralize endogenous peroxidase activity. 25 mL of reagent is supplied.
Clontech Labs 3P Wash and Stop Solution for ELISA without Sulfuric Acid | 5 x 96 Rxns
This product contains 10X PBS and Tween20 for preparing the wash solution and Stop Solution for stopping the peroxidase reaction, which are used together with TaKaRa Bio' s ELISA Kits. Although the Stop Solution is not sulfuric acid (H2SO4) -based, it can be used like 1N H2SO4. Use the prepared wash solution and Stop Solution according to the user manual for each ELISA Kit.