Protein Method Blocking Buffers
LICOR Intercept (PBS) Blocking Buffer (3 x 500 mL)
Intercept (PBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. It contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies, it is an excellent choice for biotin-streptavidin detection and it is suitable for a wide range of applications, including Western blots, In-Cell Western™ assays, and protein arrays. Intercept (PBS) Blocking Buffer is a ready-to-use blocker formulation in PBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon-FL PVDF and nitrocellulose membranes.
Rockland Immunochemicals BLOTTO Immunoanalytical Grade (Non-Fat Dry Milk) - B501-0500
Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to the addition of primary antibody.
TRIBIOSCIENCE INC 10% BSA Blocking PBS Solution, Sterile, 200 ML
Bovine Serum Albumin (BSA) Solution is formulated with bovine serum albumin Fraction V in 1x PBS (pH 7.2-7.6). It contains 2% bovine serum albumin Fraction V (20 mg/mL) in the buffer. The solution can be used for in vitro assays of molecular biology and protein assay.
Nacalai USA Inc BLOCKING ONE, 500ML
Blocking One is superior to conventional blocking solutions. The preservative does not affect the enzyme activity of POD or ALP. Only simple refrigerator storage is necessary, even after opening the bottle.
Sigma Aldrich Fine Chemicals Biosciences Carbonate-Bicarbonate Buffer capsule | 50CAP
Carbonate-Bicarbonate Buffer capsule | 50CAP
LICOR Intercept T20 (TBS) Antibody Diluent (3 x 500)
Intercept T20 (TBS) Antibody Diluent improves the specificity of the primary and secondary antibodies, reducing off-target effects. The diluent contains Intercept (TBS) Blocking Buffer in Tris-buffered saline (TBS) preformulated with Tween 20. Since there’s no need to mix the diluent yourself, it saves you time and reduces potential variation. Intercept T20 (TBS) Antibody Diluent contains a 0.05% concentration of Tween 20. It contains no sodium azide and is stored at 4 °C.
Surmodics Inc Phosphate Buffered Saline/Casein Block & Diluent-5X, 1L
BioFX™ Phosphate Buffered Saline/Casein Block and Diluent-5X Concentrate minimizes non-specific interactions of the secondary reagents with each other and the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. The solution is ready for dilution to specific application requirements.
Rockland Immunochemicals Blocking Buffer for Fluorescent Western Blotting - MB-070
Western Blot Blocking Solution is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.
Boston Bioproducts Inc Tris Buffered Saline-Tween, TBST (10X, with 0.5% Tween-20, pH 7.4) - 4x1L
10X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Boston Bioproducts Inc TBST 20X W TWEEN-20, 1% Tween-20, pH 7.4, Wash buffer, 4 x 1 L
TBST 20X W TWEEN-20, 1% Tween-20, pH 7.4, Wash buffer, 4 x 1 L
LAB BIOREAGENTS StrongBlock III | 500mL
Lab Bioreagents Buffers have been developed through the screening of >2000 compounds with the support of machine learning (AI) and optimized through kinetic and dynamic binding tests. StrongBlock (I, II, III) are newly developed blocking buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The blocking buffer is to block excess binding sites on the micro-plate for reducing non-specific binding signal, by which the assay sensitivity and specificity are improved. As many factors can influence nonspecific binding, there is no single blocking buffer that is ideal for each assay. For example, depending on the recognition of detection probes from different assays, one individual blocking buffer may cross-react with detection probe. StrongBlock (I, II, III) are a variety of buffers designed by different blocker components.
Sigma Aldrich Fine Chemicals Biosciences Casein Blocking Buffer 10x, 500 mL
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
INNOVEX BIOSCIENCES UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 5 ML
UNIVERSAL Fc RECEPTORS BLOCKER NON-ANTIBODY BASED 5 ML
Teknova 1X PBS with 0.05% Tween-20, pH 7.4. 20L Carboy, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.