Direct PCR Reagents and Kits
Applied Biosystems™ CLA GlobalFiler™ PCR Amplification Kit
The CLA (Cell Line Authentication) GlobalFiler PCR Amplification Kit simplifies the amplification step during authentication of cell lines.
Applied Biosystems™ CLA IdentiFiler™ Plus PCR Amplification Kit
The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines.
Viagen Biotech Inc PCR LYSIS RGNT(MOUSE EAR
DirectPCR Lysis Reagent (Ear) 50 ml. Viagen DirectPCRa DNa Extraction System is a single-tube system for rapid preparation of DNa from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech Inc. allow the resulting DNa extracts to be compatible with genomic PCR for genotyping. Crude extracts of biological samples are not compatible with many molecular biology-grade reactions such as polymerase chain reaction (PCR), in part due to inhibitors contained in crude extracts. The DirectPCR reagents not only mediate the rapid lysis of biological samples but also contain inhibitors that effectively suppress the inhibitory activities of crude lysates for PCR amplification, while maximally maintaining the integrity of released genomic DNa. The patent-pending simple procedure completely eliminate any solution transfer or tube-opening steps, providing you with substancial extra time.
Viagen Biotech Inc DirectPCR Lysis Reagents
DirectPCR Lysis Reagents are cell lysis reagents designed for direct PCR amplification from a variety of sample types including blood, bacteria, yeast, tissue, plants, and insects. The reagents lyse cells and degrade proteins while protecting nucleic acids from degradation. They allow PCR amplification directly from crude samples without DNA extraction or purification. The reagents are available in several formulations optimized for different sample types. They provide a fast and simple method for PCR amplification from samples containing proteins, lipids, polysaccharides, or other PCR inhibitors.
Agilent Technologies FUSION DNA POLYMERASE
Provides superior yield for routine PCR amplification and successful amplification of difficult GC rich targets. dNTPs included.
Viagen Biotech Inc PROTEINASE K SOLUTION
Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolysed is a tetrapeptide. DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biological samples are processed. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 ul Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR Reagent Protocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for ~24 hrs.
Agilent Technologies 40 RXN PFUULTRA II FUSION HS
PfuUltra II Fusion HS DNA Polymerase; 40 rxn; 1mL; Store at -20 deg.C; Extended length capability up to 19 kb
Apexbio Technology LLC 1STEP COMP CELL PREP KIT 200T
1STEP COMP CELL PREP KIT 200T APEXBIO TECHNOLOGIES
BIOSEARCH TECHNOLOGIES INC (Lucigen) EconoTaq PLUS 2X Master Mix, 500 RxnsDependable Taq polymerase performance.Choice of reaction buffers, with or without MgCl2.Non-proofreading Polymerase
EconoTaq PLUS GREEN & EconoTaq PLUS 2X Master Mixes offer Performance, Convenience, Reliability, and Value for Routine PCR. More effective than other Taq master mixes. EconoTaq PLUS and PLUS GREEN 2X Master Mixes contain a proprietary PCR enhancer, so they can successfully amplify templates that fail to amplify with other PCR master mixes. High density reaction buffer enables direct gel loading following PCR. Excellent results in routine PCR. EconoTaq PLUS GREEN & EconoTaq PLUS contain high purity, high activity EconoTaq DNA Polymerase for reliable amplification of templates up to 5 kb. Amplify challenging GC-rich templates. These 2X Master Mixes can be cycled up to 98C to amplify challenging GC-rich templates others cannot. Convenient tracking dyes in EconoTaq PLUS GREEN. On a 1% agarose gel, the blue dye migrates at the same rate as a 5 kb DNA fragment, and the yellow dye migrates at 75 bp.
Viagen Biotech Inc DIR PCR LYSIS RGNT(MOUSE TAIL
Viagen DirectPCR� DNA Extraction System is a single-tube system for rapid preparation of DNA from mouse tails, ear pieces, yolk sac, and culture cells. The patent-pending components developed by scientists at Viagen Biotech Inc. allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. DirectPCR� system offers advantages over conventional protocols that include: � Time saving: Less hands-on time. Crude tail lysates for PCR. � Money saving: Cost-effective reactions. � Safe: No organic reagents. � Environmental: Less waste (organic reagents, tubes, tips, etc...) � Reliable and efficient: Virtually 100% success rate with high yields. 50ml (250 mouse tails).
Agilent Technologies PFU ULTRA HIGH FIDELITY DNA
PfuUltra High-Fidelity DNA Polymerase AD features a reformulated buffer system for increased economy with the same high performance as the original PfuUltra DNA polymerase for robust, ultra-high-fidelity PCR.
Beckman Coulter AMPure XP, 60 mL
A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration, AMPure XP can be easily used in manual and automated 96- or 384-well formats.
Beckman Coulter AMPure XP, 5 mL
A highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration, AMPure XP can be easily used in manual and automated 96- or 384-well formats
Viagen Biotech Inc DIR PCR LYSIS RGNT(MOUSE TAIL)
DirectPCR Lysis Reagent (Mouse Tail) 100ml (Up to 500 Tails). The system is a single-tube system for rapid preparation of DNA from mouse tails. The patent-pending components allow the resulting DNA extracts to be compatible with genomic PCR for genotyping. Crude extracts of biological samples are not compatible with many molecular biology-grade reactions such as polymerase chain reaction (PCR), in part due to inhibitors contained in crude extracts. The DirectPCR reagents not only mediate the rapid lysis of biological samples but also contain inhibitors that effectively suppress the inhibitory activities of crude lysates for PCR amplification, while maximally maintaining the integrity of released genomic DNA. The patent-pending simple procedure completely eliminates any solution transfer or tube-opening steps, providing you with substantial extra time. Stable for 12 months at 4Deg C / Ships at room temperature.
Zymo Research Corporation DNA Clean & Concentrator™-5 (200 Preps) w/ Zymo-Spin™ IC Columns (Capped)
The DNA Clean & Concentrator-5 (DCC-5) can also be used for post-RT cDNA clean-up and purification of sequencing-ready DNA from M13 phage. Simply add the specially formulated DNA Binding Buffer to your sample and transfer the mixture to the supplied Zymo-Spin™ Column. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin column technology to yield DNA that is free of salts and contaminants injust 2 minutes. The purified DNA is ideal for DNA ligation, sequencing, labeling, PCR, microarray, transfection, transformation, and restriction digestion procedures.