Hot Start PCR Reagents and Kits
Sigma Aldrich Fine Chemicals Biosciences PCR Buffer Without MgCl2 1
The PCR buffer without MgCl2 in combination with the MgCl2 stock SOLUTIONon is used for the individual adjustment of the Mg2 concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200muM each. In many cases however it is required to titrate the optimal Mg2 concentration to increase the specificity and yield of the PCR reaction.
New England Biolabs, Inc. OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer – 100 reactions (50 µl vol)
OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep Vent DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient Quick-Load master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands
New England Biolabs, Inc. OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer – 500 reactions (50 µl vol)
OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer is an optimized, ready-to-use blend of Taq and Deep Vent DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to routine PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient Quick-Load master mix formulation contains dNTPs, MgCl2, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands
New England Biolabs, Inc. OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer – 500 reactions (50 µl vol)
OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer is an optimized, ready-to-use blend of Taq and Deep Vent DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies, and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands.
Roche Diagnostics KAPA HiFi HotStart Uracil+ ReadyMix Kit (50 rxn), 50 x 50 uL reactions
KAPA HiFi HotStart Uracil+ ReadyMix (2X) contains DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 2.5 mM). *This item is non returnable.
Roche Diagnostics KAPA HiFi HotStart Uracil+ ReadyMix Kit (250 rnx), 250 x 50 uL reactions
KAPA HiFi HotStart Uracil+ ReadyMix (2X) contains DNA Polymerase, reaction buffer, dNTPs and MgCl2 (at a final concentration of 2.5 mM). *This item is non returnable.
LAMDA BIOTECH INC SafeStain 2x PCR Master Mix
The SafeStain™ 2X PCR Master Mix is an advanced research reagent combining PCR master mix with DNA visualization capability in a single solution. The formulation includes Taq Plus™ DNA polymerase, optimized concentrations of dNTPs, Mg2+, and PCR reaction buffer components.
Alkali Scientific Radiant™ 2X RED Hot-Start Taq Mix 200 Reactions 5 x 1mL
RadiantTM Red 2X Hot-Start Taq Mastermix is a ready-to-use, robust PCR mix containing our highly purified Hot-Start Taq DNA Polymerase, reaction buffer, ultra-pure dNTPs, MgCl2 and PCR enhancers. RadiantTM Red 2X Hot Start Taq Mastermix is optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian & any crude genomic DNA and is ideal for screening, colony PCR, high-throughput PCR and genotyping of problematic GC-rich and AT-rich sequences. The Hot-Start technology is based on a new-generation PCR buffer formulation in conjunction with a proprietary antibody-mediated chemistry. RadiantTM Red 2X Taq Hot-Start Mastermix is provided at 2× concentration and used at 1× concentration by adding template, primer, and H2O. • Intert red dye will not inhibit PCR reaction & is used as a downstream Gel Loading Dye when running gel • No change in PCR cycling conditions when in supplied Red format. Red Dye cycles within PCR machine • New-gener
Alkali Scientific Radiant™ 2X RED Hot Start Taq Mix 1000 Reactions 25 x 1mL
RadiantTM Red 2X Hot-Start Taq Mastermix is a ready-to-use, robust PCR mix containing our highly purified Hot-Start Taq DNA Polymerase, reaction buffer, ultra-pure dNTPs, MgCl2 and PCR enhancers. RadiantTM Red 2X Hot Start Taq Mastermix is optimized for the sensitive DNA amplification of a wide range of DNA templates including complex mammalian & any crude genomic DNA and is ideal for screening, colony PCR, high-throughput PCR and genotyping of problematic GC-rich and AT-rich sequences. The Hot-Start technology is based on a new-generation PCR buffer formulation in conjunction with a proprietary antibody-mediated chemistry. RadiantTM Red 2X Taq Hot-Start Mastermix is provided at 2× concentration and used at 1× concentration by adding template, primer, and H2O. • Intert red dye will not inhibit PCR reaction & is used as a downstream Gel Loading Dye when running gel • No change in PCR cycling conditions when in supplied Red format. Red Dye cycles within PCR machine • New-gener
101 BIO 1-Drop PCR Master Mix (with dye)
User can squeeze one drop of PCR Master mix into PCR tube/plate, add template and primers, and then can run PCR reaction. This PCR Master Mix contains Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer. This unique Mix recipe makes the system very reliable, and tolerant to most of the PCR condition (concentration of the components). The Taq Polymerase in the PCR Master Mix is Hot Start polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton. This prevents the formation of misprimed products and primer dimers at ambient temperatures. So no need to prepare the reactions on ice. The Polymerase is activated by a 10 minutes, 95°C incubation.
101 BIO 2x GoldStar Best PCR Master Mix (with dye), (1 mL, 100 rxn, 20 ul/rxn)
2x GoldStar Best PCR Master Mix (with dye) is a convenient premixed 2x concentrated solution for PCR which include GoldStar Best DNA Polymerase, PCR Buffer, Mg2+, dNTPs, PCR Stabilizer and PCR Intensifier.The polymerase has a 5´→3´ DNA polymerase and a 5´→3´ exonuclease activity. It also possesses 3´→5´ exonuclease (proofreading) activity. The polymerase has a higher amplification efficiency and lower mispairing rate than routine Taq DNA polymerase.The GoldStar Best is a high reliable hotstart polymerase, which activity is inhibited at ambient temperatures by the chemical modificaiton. This prevents the formation of misprimed products and primer dimers at ambient temperatures. GoldStar Best Polymerase is activated by a 10 minutes, 95°C incubation. Optimized buffer system promotes the amplification of target fragment with high fidelity, high specificity, high amplification efficiency and high sensitivity.
New England Biolabs, Inc. WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) - 100 rxn
The WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) is designed to provide a simple one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. This kit includes the WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution and a vial of LAMP Fluorescent Dye an intercalating dye provided at 50X concentration that can be used to track a LAMP reaction in real-time by fluorescence measurement. The inclusion of thermolabile UDG prevents carryover contamination where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions.
New England Biolabs, Inc. WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) - 500 rxn
The WarmStart Fluorescent LAMP/RT-LAMP Kit (with UDG) is designed to provide a simple one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. This kit includes the WarmStart Multi-Purpose LAMP/RT-LAMP 2X Master Mix (with UDG) which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized buffer solution and a vial of LAMP Fluorescent Dye an intercalating dye provided at 50X concentration that can be used to track a LAMP reaction in real-time by fluorescence measurement. The inclusion of thermolabile UDG prevents carryover contamination where unintended product of a previous amplification can serve as the substrate of a subsequent reaction. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions.
101 BIO 2x Es Taq Master Mix (with dye) (1 mL, 100 rxn, 20 ul/rxn)
This Master Mix contains Es Taq DNA Polymerase, PCR buffer, Mg2+, dNTP, PCR stabilizer and PCR enhancer. The concentration is 2x.Es Taq DNA Polymerase possesses 5´→3´ DNA polymerase and 5´→3´ exonuclease activity. The polymerase has the high amplification efficiency and low mismatching as Taq DNA polymerase and high fidelity of Pfu DNA polymerase. Es Taq Polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for T/A cloning. The amplification range of Es Taq is ~ 6 kb.This unique Master Mix recipe makes the system very reliable. More than 98% of PCR reaction can get successful amplification during the first try. It also works well on complicate templates.The Master Mix contains dye, and can directly run electrophoresis after PCR reaction.
New England Biolabs, Inc. OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer – 100 reactions (50 µl vol)
OneTaq Hot Start Quick-Load 2X Master Mix with GC Buffer is an optimized, ready-to-use blend of Taq and Deep Vent DNA Polymerases combined with an aptamer-based inhibitor. This enzyme blend is ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies, and cDNA products. The 3' to 5' exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase. The hot start nature of the enzyme offers convenience with decreased interference from primer dimers and secondary products. The convenient master mix formulation contains dNTPs, MgSO4, buffer components and stabilizers as well as two commonly used tracking dyes for DNA gels. On a 1% agarose gel in 1X TBE, Xylene Cyanol FF migrates at ~4 kb and Tartrazine migrates at ~10 bp. Both dyes are present in concentrations that do not mask co-migrating DNA bands.