5 ML. CTL anti-aggregate Washa, c 20x Stabilizes PBMC during thawing and prevents cell loss through aggregation. Cells that die during freeze-thawing release DNa strands that can cause cell clumping and aggregation. Therefore, the use of DNase-containing wash solutions is recommended while thawing PBMC. Moreover, cells show increased metabolic activity after thawing as they compensate for the stress reaction - this needs to be accounted for by using a nutrient-rich medium. CTL anti-aggregate Washa, c Supplement has been specifically formulated for optimal PBMC recovery and functionality after thawing. It contains injection-grade water supplemented with amino acids, vitamins, inorganic salts, and human serum albumin (HSa) in a proprietary composition, saturated and unsaturated fatty acids, and sugars in a ready-to-use formulation, providing a balanced environment for PBMC. For each vial of PBMC to be thawed, 1 vial of CTL anti-aggregate Washa, c Supplement 20x is needed.