Dissociation Reagents
Dissociation Reagents
STEMCELL Technologies STEMdiff™ Neural Rosette Selection Reagent, Size: 100 mL
STEMdiff™ Neural Rosette Selection Reagent is an enzyme-free reagent for the selective detachment of neural rosette clusters from adherent neural aggregates previously generated from human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium, without manual scraping. Collecting and re-plating rosette clusters after incubation with STEMdiff™ Neural Rosette Selection Reagent will yield highly pure populations of neural progenitor cells, which can be further sub-cultured as single cells.
STEMCELL Technologies STEMdiff™ Cardiomyocyte Dissociation Kit, Size: 1 Kit
STEMdiff™ Cardiomyocyte Dissociation Kit includes STEMdiff™ Cardiomyocyte Dissociation Medium and STEMdiff™ Cardiomyocyte Support Medium. STEMdiff™ Cardiomyocyte Dissociation Medium can be used to harvest cardiomyocytes that have been differentiated from human pluripotent stem cells (hPSCs) using STEMdiff™ Ventricular Cardiomyocyte Differentiation Kit (Catalog #05010) or STEMdiff™ Atrial Cardiomyocyte Differentiation Kit (Catalog #100-0215) and maintained in STEMdiff™ Cardiomyocyte Maintenance Medium (Catalog #05010/05020). STEMdiff™ Cardiomyocyte Support Medium reduces stress on these cardiomyocytes during harvesting and replating, maintaining their viability and functional capacity for downstream applications and analyses.
STEMCELL Technologies NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat), Size: 1 Kit
NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) is recommended for the enzymatic digestion and dissociation of adult mouse and rat central nervous system (CNS) tissue. NeuroCult™ Enzymatic Dissociation Kit has been optimized so that the entire procedure is reproducible, fast, and yields high cell numbers and viabilities. The resulting single-cell suspension is ready for immediate use in downstream applications.
Lonza Walkersville MycoAlert PLUS Mycoplasma Detection Kit (50 Tests)
Lonza’s MycoAlert Mycoplasma Detection Assays are selective biochemical tests to detect Mycoplasma contamination in cell cultures. They exploit the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells. Benefits:Easy assay: Simply add two reagents to the culture supernatant and perform two luminescence readingsResults in < 20 minEasy interpretation of resultsApplications:Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)Suited for tube luminometers, plate luminometers and multifunctional readersContent:1 x 5 mL MycoAlert PLUS Reagent (lyophilized)1 x 5 mL MycoAlert PLUS Substrate (lyophilized)1 x 10 mL MycoAlert PLUS Assay Buffer
Sciencell Research Laboratories Trypsin/EDTA Solution, 0.05%
Trypsin is a serine protease produced in the pancreas. Ethylenediaminetetraacetic acid (EDTA) is a chelating agent which sequesters metal ions such as Ca+2 and Mg+2,enhancing the trypsinization reaction. 0.05% Trypsin/EDTA solution (T/E) is a sterile, phosphate and HEPES-buffered saline solution with a pH of 7.4 at room temperature. It contains 0.05% trypsin and 0.5 mM EDTA.
AbD Serotec Mycoplasma Removal Agent, 5 mL
Mycoplasma Removal Agent; Developed for cell culture; 5mL; Non toxic; Prevent initial infection of cells in culture by mycoplasma; Cytotoxicity of MRA is low and cell toxicity is rare; Stable at RT
Charles River Laboratories DAILY WASH AND RINSE KIT
For use with Celsis rapid detection systems. Please consult your Celsis representative for additional information.
Promocell GmbH PromoCell Macrophage Detachment Solution
Macrophages are strongly adherent and are not effectively dislodged from culture surfaces by standard enzymatic cell dissociation reagents. Indeed, these cells are highly sensitive to improper dissociation procedures. The PromoCell Macrophage Detachment Solution is chemically defined and non-enzymatic. It was especially designed for the gentle release of adherent macrophages and guarantees the best possible cell viability, even after prolonged exposure times. Unlike enzyme-based solutions, the PromoCell Macrophage Detachment Solution does not alter cell surface proteins and neutralization is not necessary.
Amsbio LLC Detachin Cell Detachment Solution
Detachin™ Cell Detachment Solution is a superior alternative to Trypsin/EDTA for gently detaching adherent cells from in vitro growth vessels. Detachin™ provides quick, gentle, and effective detachment of a wide variety of adherent cells, including primary cells, from all known tissue culture plastic ware. It contains protease and collagenase activities in an isotonic, phosphate buffer solution with EDTA.
Cell Applications Inc i-Human Neural Stem Cell Dissociation Solution
i-Human Neural Stem Cell Dissociation Solution
STEMCELL Technologies ACCUMAX™, Size: 100 mL
ACCUMAX™ is a cell detachment solution of proteolytic and collagenolytic enzymes. It is useful for creating single-cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion-coated plasticware. ACCUMAX™ does not contain mammalian- or bacterial-derived products.Each lot of ACCUMAX™ is tested for sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides), and cell detachment from tissue culture plastic.
STEMCELL Technologies ReLeSR™, Size: 500 mL
ReLeSR™ is an enzyme-free reagent for dissociation and passaging of human embryonic stem (ES) or induced pluripotent stem (iPS) cells as aggregates without manual selection or scraping. Passaging human ES/iPS cells with ReLeSR™ easily generates optimally-sized aggregates, while eliminating the hassle and variability associated with manual manipulation. By eliminating the need for scraping, ReLeSR™ enables the use of culture flasks and other closed vessels, thus facilitating culture scale-up and automation. ReLeSR™, 500 mL, is now also manufactured following relevant cGMPs under a certified quality management system to ensure the highest quality and consistency for reproducible results.To request a Letter of Authorization (LOA) for ReLeSR’s (Catalog #100-0484) Drug Master File, click here (https://www.stemcell.com/loa-request-dmf).
Bioworld Neutral Dissociator, pH 7.0, 50 mL
Neutral Dissociator, pH 7.0 Two reagents are provided to dissociate your antigen-antibody complex in a column. Dialyze to desalt after dissociating under denaturing conditions with neutral dissociator.