Additional Selective and Differential Cell Culture Media
NEOGEN Tomato Juice Agar, 500 g
Tomato Juice Agar is used for the cultivation of lactobacilli in a laboratory setting. Tomato Juice Agar is not intended for use in the diagnosis of disease or other conditions in humans. In 1925, Mickle and Breed reported the use of tomato juice in culture media used for cultivating lactobacilli. Kulp investigated tomato juice on bacterial development and found growth of L. acidophilus was enhanced. Tomato Juice Agar, prepared according to Kulp and White’s modification, is especially useful in cultivating L. acidophilus from foodstuffs.For more information visit https://www.neogen.com/solutions/Microbiology/tomato-juice-agar
NEOGEN SIM Medium, 500 g
SIM Medium is used for the differentiation of microorganisms on the basis of hydrogen sulfide production, indole production, and motility in a laboratory setting. SIM Medium is not intended for use in the diagnosis of disease or other conditions in humans. Semisolid media have been used extensively in the determination of bacterial motility. The production of hydrogen sulfide, indole formation, and motility are useful diagnostic tests in the identification of Enterobacteriaceae, especially Salmonella spp. and Shigella spp. In 1940, Sulkin and Willett demonstrated motility, hydrogen sulfide production, and carbohydrate fermentation by members of Salmonella and Shigella groups. They called attention to the “brush-like growth” or motility of the typhoid organisms. Greene et al. used SIM Medium to detect motility in a large series of cultures of typhoid organisms.For more information visit https://www.neogen.com/solutions/Microbiology/sim-medium
NEOGEN Terrific Broth, Modified, 500 g
Terrific Broth, Modified is used with glycerol in cultivating recombinant strains of Escherichia coli in a laboratory setting. Terrific Broth, Modified is not intended for use in the diagnosis of disease or other conditions in humans. Terrific Broth, Modified was developed by Tartoff and Hobbs to increase yields of plasmid DNA from transformed E. coli and is a popular choice of media available for plasmid preps. While cells grown in Terrific Broth, Modified can grow slower than other liquid media, the final cell density and plasmid yields are 2 – 4 fold higher. The higher yield is a result of the increased concentration of Enzymatic Digest of Casein and Yeast Extract in this formula. Glycerol is used as a carbohydrate source, and unlike glucose, is not fermented to acetic acid.For more information visit https://www.neogen.com/solutions/Microbiology/terrific-broth-modified
NEOGEN m-HPC Agar, 500 g
m-HPC Agar is used for the enumeration of heterotrophic organisms in water by the membrane filtration technique in a laboratory setting. m-HPC Agar is not intended for use in the diagnosis of disease or other conditions in humans.m-HPC Agar, also known as m-Heterotrophic Plate Count Agar, was developed by Taylor and Geldreich in 1979. Monitoring the levels of heterotrophic bacteria, those microorganisms that can grow on minimal organic nutrients, is currently performed to indicate problems with treatment methods within distribution systems. There is evidence that heterotrophic bacteria are not hazardous to healthy individuals, but may be opportunistic pathogens capable of infecting individuals with impaired immune systems.m-HPC Agar is used in standard methods for the detection of heterotrophic bacteria using the membrane filtration technique.For more information visit https://www.neogen.com/solutions/Microbiology/m-hpc-agar
NEOGEN Mycobiotic Agar, 500 g
Mycobiotic Agar is used for the selective isolation of fungi in a laboratory setting. Mycobiotic Agar is not intended for use in the diagnosis of disease or other conditions in humans. The value of selective media for initial cultivation of pathogenic fungi has been demonstrated by numerous investigators. Historically, media for fungi generally relied on an acid pH to make the media less suitable for growth of many bacteria. Recently developed media use neutral or slightly alkaline reactions, antibiotics, bile salts, and dyes as selective agents against bacteria. Mycobiotic Agar is an excellent basal medium and antifungal agents, cycloheximide and chloramphenicol, are added to study their affect on fungi. This medium is proven useful in the isolation of dermatophytes and other fungi from samples.For more information visit https://www.neogen.com/solutions/Microbiology/mycobiotic-agar
NEOGEN Harlequin Tryptone Bile Glucuronide Agar (TBX), 500 g
Harlequin Tryptone Bile Glucuronide Agar (TBX) is used for the enumeration of E. coli in food and animal feed and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptone Bile Glucuronide Agar is recommended by ISO 16649-1:2001, ISO 16649-2:2001 and ISO 16649-3:2015 for enumeration of β-glucuronidase positive Escherichia coli in products intended for human consumption or for the feeding of animals. The medium contains tryptone as a source of nitrogen and amino acids, bile salts act as a selective against Gram-positive organisms and agar is the gelling agent. 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-glucuronide) is a chromogen for the detection of the β-D-glucuronidase enzyme which is present in most E. coli* but not other coliforms.*96-97% of E.coli are β-D-glucuronidase positive. A notable exception is E. coli O157:H7 For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-tryptone-bile-glucuronide-agar-tbx
NEOGEN Acuferm Peptone, 10 kg
For more information visit https://www.neogen.com/solutions/Microbiology/acuferm-peptone
NEOGEN Acuferm Neopeptone, 10 kg
For more information visit https://www.neogen.com/solutions/Microbiology/acuferm-neopeptone
NEOGEN Harlequin Chromogenic Coliform Agar, 500 g
Chromogenic Coliform Agar (CCA) is for the enumeration of Escherichia coli and Coliforms in water and is not intended for use in the diagnosis of disease or other conditions in humans. Chromogenic Coliform Agar (CCA) is recommended by ISO 9308-1 for the simultaneous detection and enumeration of β-glucuronidase-positive Escherichia coli and β-D-galactosidase positive coliform bacteria from water samples using membrane filtration. Peptone and yeast extract provide a source of nitrogen and essential vitamins. Sodium chloride maintains the osmotic balance and the phosphates buffer the media. Sodium Pyruvate, Sorbitol and Tryptophan aid in the recovery and detection of the target organisms. Tergitol™ 7 is a surfactant that acts as a selective agent. The dual chromogens are incorporated to differentiate between E. coli and coliforms based on their enzymatic activity.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-chromogenic-coliform-agar
NEOGEN Acuferm Neopeptone, 500 g
For more information visit https://www.neogen.com/solutions/Microbiology/acuferm-neopeptone
NEOGEN Harlequin Tryptone Bile Glucuronide Agar (TBX), 5 kg
Harlequin Tryptone Bile Glucuronide Agar (TBX) is used for the enumeration of E. coli in food and animal feed and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptone Bile Glucuronide Agar is recommended by ISO 16649-1:2001, ISO 16649-2:2001 and ISO 16649-3:2015 for enumeration of β-glucuronidase positive Escherichia coli in products intended for human consumption or for the feeding of animals. The medium contains tryptone as a source of nitrogen and amino acids, bile salts act as a selective against Gram-positive organisms and agar is the gelling agent. 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-glucuronide) is a chromogen for the detection of the β-D-glucuronidase enzyme which is present in most E. coli* but not other coliforms.*96-97% of E.coli are β-D-glucuronidase positive. A notable exception is E. coli O157:H7 For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-tryptone-bile-glucuronide-agar-tbx
NEOGEN Harlequin mLGA, 500 g
Membrane Lactose Glucuronide Agar (mLGA) is a medium for the detection and identification of E. coli and coliforms from water samples and is not intended for use in the diagnosis of disease or other conditions in humans.Traditionally, membrane Lauryl Sulphate Broth (mLSB) has been used as the standard media for isolating coliforms (including E. coli) from water potentially contaminated with sewage. Harlequin membrane Lactose Glucuronide Agar (mLGA) is a modification of mLSB aimed at reducing costs by reducing the number of filters used per test sample and aiding in the recovery and identification of coliforms and E. coli. The medium has been modified from the mLSB formulation by the incorporation of X-glucuronide (BCIG), sodium pyruvate and agar.*96-97% of E. coli strains positive. A notable exception is E. coli O157:H7.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-mlga
Lonza Walkersville SAGM Small Airway Epithelial Cell Growth Medium SingleQuots Supplements and Growth Factors
SAGM SingleQuot Kits are components sold separately and does not include the basal medium. These are part of the full SAGM Small Airway Epithelial Cell Growth Medium BulletKit (catalog number CC-3118) which consists of basal medium and supplements packaged together. SAGM BulletKit.SAGM BulletKit is serum-free and has been optimized to grow human small airway epithelial cells (SAEC).Content:1 x Orange Cap Vial with BPE 2.0 mL1 x Lilac Cap Vial with Insulin 0.5 mL1 x Natural Cap Vial with Hydrocortisone 0.5 mL1 x Red Cap Vial with GA-1000 0.5 mL1 x Amber Vial with Retinoic Acid 0.5 mL1 x Bottle with Fatty Acid Free BSA 5 mL1 x Natural Cap Vial with Transferrin 0.5 mL1 x Amber Vial with Triiodothyronine 0.5 mL1 x Amber Vial with Epinephrine 0.5 mL1 x Green Cap Vial with hEGF 0.5 mL
Research Products International Corp Gamborg's B5 Medium, Powder, Makes 10 Liters
Basal salt mixture containing micro and macro elements as described by Gamborg, et al. (1968). Package contains 3.05 grams of powder per liter of medium.