Cell Lines and Cell Cultures
Eurofins Discoverx ChemiScreen MC2 Melanocortin Receptor Stable Cell Line, 2 vials. (HTS021C)
The ChemiScreen MC2 Melanocortin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. adrenocorticotropin) based activation of MC2R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses MC2R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of MC2R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
ATCC Salmonella enterica; subsp. enterica; serovar typhimurium
Salmonella enterica subsp. enterica strain TA 1535 is a whole-genome sequenced bacterium with applications in enteric disease research, infectious disease research, and zoonotic disease research. This strain is serotype I 4,5,12:i:1,2 and is the HisG46, rfa-, uvrB derivative of strain LT-2.
Biolegend TotalSeqTM-B0005 anti-human CD
TotalSeq™-B0005 anti-human CD80 [2D10]; Isotype: Mouse IgG1, κ; Reactivity: Human; Apps: PG; Size: 10 μg
ATCC Salmonella enterica; subsp. enterica; serovar Typhimurium; phage P22
Bacteriophage P22 is propagated in Salmonella enterica subsp. enterica serovar Typhimurium strain LT2. This organism has applications as a transducing phage for Salmonella Groups B and D.
Cell Systems Corporation Primary Human Liver Sinusoidal Microvascular Endothelial Cells (ACBRI 566 V)
Primary Human Liver Sinusoidal Microvascular Endothelial Cells (ACBRI 566 V) were initiated from normal human liver tissue. Unlike cells from other providers, Cell Systems primary cells are isolated without antibody labels for enhanced biological relevance. Each vial contains ~1 x 10^6 cells with 85-92% viability under ideal conditions. They are available at Passage 3 (<12 cumulative population doublings) and will initiate a Passage 4 cell culture in a 75 cm2 flask. Each vial of cells is shipped with Bac-Off (antibiotic) and CultureBoost (animal derived growth factors) at no additional cost. You can enhance consistency in your research program with reserved lots. Need vials in bulk? Contact your Fisher Scientific Sales representative for tailored quotes. Tight Deadline? Our cells are available for next day delivery after the purchase order is received. Accelerate Your Research! Try our Attachment Factor (4Z0-201, 4Z0-210) – innovative 5 seconds plating solution for all surfaces.
ATCC Mycoplasma synoviae; Strain WVU 1853
Mycoplasma synoviae strain WVU 1853 [NCTC 10124] was isolated from the hock joint of a chicken. This titered bacterial type strain can be used in assay development and quality control.
ATCC Quantitative Genomic; DNA from Mycoplasma; pneumoniae Strain: M129-B7
ATCC Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.
Eurofins Discoverx PathHunter U2OS rhTrkA-rhP75 Functional Assay, 2 vials. (93-1009C3)
The PathHunter U2OS rhTrkA-rhP75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated rhTrkA and rhP75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged rhTrkA and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, rhP75 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the rhesus version of the protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
ATCC Haemophilus influenzae
Haemophilus influenzae strain TD-4 was isolated in January 1984 from the expectorated sputum of a male with pneumonia. This whole-genome sequenced bacterial strain can be used in susceptibility disc testing and respiratory disease research.
Eurofins Discoverx CHO-K1 BDKRB1 Gq Cell Line, 2 vials. (95-1011C2)
The BDKRB1 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. bradykinin) based activation of BDKRB1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses BDKRB1, a naturally Gq coupled GPCR. Upon activation of BDKRB1, the resulting calcium activity can be fluorescently assayed using the Calcium No WashPLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Rhizopus microsporus; IMI 130842
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC Pseudomonas aeruginosa; PA01-LAC
Pseudomonas aeruginosa strain PAO1-LAC is a whole-genome sequenced bacterium that can be used as a transformation host for the expression vector pUCP19 (ATCC 87110).
ATCC SU-DHL-8; B Cell Lymphoma; Human (Homo sapiens)
SU-DHL-8 is a B lymphocyte cell line that was isolated in 1978 from the lymph node of a 59-year-old, White male with large cell lymphoma. It has applications in immunology research.