General Cell Lines and Cell Cultures
ATCC Thermoactinomyces vulgaris
Thermoactinomyces vulgaris strain NCIB 11364 is a thermophilic bacterium with applications in biotechnology. This strain grows aerobically at 50-55°C.
ATCC pIJ702 in; Streptomyces lividans
Cloning vector with thiostrepton resistance (tsrR) and tyrosinase production (mel+) markers.
Apexbio Technology LLC NFAT Inhibitor 249537-73-3 1mg
NFAT Inhibitor (CAS 249537-73-3) is a selective small molecule that blocks the calcineurin-dependent dephosphorylation of the nuclear factor of activated T cells (NFAT) thereby preventing its activation Unlike general calcineurin inhibitors this compound does not interfere with other calcineurin-mediated signaling pathways By inhibiting NFAT activation it reduces the expression of NFAT-dependent cytokine genes in T cells NFAT Inhibitor is valuable for studying the specific roles of NFAT signaling in immune cell function and for dissecting calcineurin-related pathways in immunological and cellular research
Eurofins Discoverx PathHunter U2OS TYRO3 Functional Assay, 2 vials. (93-1110C3)
The PathHunter U2OS TYRO3 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TYRO3 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TYRO3 and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx cAMP Hunter CHO-K1 CXCR2 (IL8RB) Gi Cell Line, 2 vials. (95-0074C2)
The cAMP Hunter CXCR2 (IL8RB) Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL8/IL8) based activation of CXCR2 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR2, a naturally Gi/Go coupled GPCR. Upon activation of CXCR2, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiScreen NK1 Tackykinin Receptor Stable Cell Line, 2 vials. (HTS080C)
The ChemiScreen NK1 Tachykinin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. tachykinins) based activation of TACR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses TACR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of TACR1, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
STEMCELL Technologies Human Whole Peripheral Blood Collected with ACDA, Fresh, Size: 60 mL
Normal whole peripheral blood (PB) is obtained from healthy donors using Institutional Review Board (IRB)-approved consent forms and protocols. Acid citrate dextrose, solution A (ACDA), Sodium Heparin (Na-Hep), Ethylenediaminetetraacetic acid (EDTA), or Citrate-phosphate-double-dextrose (CP2D) is the anticoagulant.Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information and to inquire about donor selection or information including BMI category, smoking status, ethnicity, etc.Browse our Frequently Asked Questions (FAQs) (https://www.stemcell.com/primary-cells-faq) on Primary Cells.
ATCC pRS426 phagemid; in E. coli
This is a YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. It can be used to produce ssDNA and it contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. It also contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.4 +/- 1.4% of progeny per doubling.
Eurofins Discoverx U2OS GRM1 Gq Cell Line, 2 vials. (95-1021C3)
The GRM1 Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. L-glutamate) based activation of GRM1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses GRM1, a naturally Gq coupled GPCR. Upon activation of GRM1, the resulting calcium activity can be fluorescently assayed using the Calcium No WashPLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PrecisION hKv4.3/hKChIP2 Recombinant Stable Cell Line, 2 vials. (CYL3069)
The PrecisION hKv4.3/hKChIP2 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Kv4.3/KChIP2 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Kv4.3/KChIP2 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx Ready-to-Assay NOP/ORL1 Opioid Receptor Frozen Cells, 2 Vials of Cells. (HTS040RTA)
The Ready-to-Assay NOP/ORL1 Opioid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. orphanin FQ) based activation of OPRL1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses OPRL1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of OPRL1, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.