General Cell Lines and Cell Cultures
iQ Biosciences Purified Human CD8 T Cells, 10M cells/vial
High-quality, high-viability (> 90%) human CD8 T cells are purified from PBMCs of healthy blood donors sourced from a world-renowned blood center. Highly enriched (typically > 90%) post-selection purity achieved via magnetic isolation. All orders come with an iQ Certificate of Analysis.
Lonza Walkersville D-HBE-CF – Diseased Human Bronchial/Tracheal Epithelial Cells – Cystic Fibrosis
Cystic fibrosis is a disease that causes thick, sticky mucus to build up in the lungs, digestive tract, and other areas of the body. It is one of the most common chronic lung diseases in children and young adults. The disease is the most common, deadly, inherited disorder affecting Caucasians in the United States. The disease eventually worsens to the point where the person is disabled. Today, the average life span for people with CF who live to adulthood is approximately 37 years, a dramatic increase over the last three decades.Benefits:Test negative for mycoplasma, bacteria, yeast, and fungiHIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lotsA Certificate ofAnalysis is provided for each cell lot purchased Content:1 x Cryopreserved ampule of D-HBE-CF containing ≥500,000 cells
Alomone Labs, LTD Alomone Labs Polyclonal Antibody, Rabbit, Human, Mouse, Rat, Unconjugated
This polyclonal antibody targets CRLR (Calcitonin Receptor-Like Receptor), a receptor for calcitonin gene-related peptide type 1. Activity is mediated by G proteins, activating adenylyl cyclase. CRLR is expressed in brain, lung, blood vessel, liver, and intestinal tract.
- Host/Isotype: Rabbit IgG
- Reactivity: Human, mouse, and rat
- Immunogen: Peptide from human Calcitonin Receptor-Like Receptor (extracellular, N-terminus).
- Conjugate: Unconjugated
- Form: Lyophilized
- Concentration: 0.8 mg/mL
- Amount: 160 μg
- Purification: Antigen affinity chromatography
- Storage buffer: PBS, pH 7.4, with 1% BSA and 0.05% sodium azide
- Applications: WB (1:200), IHC (F) (1:200), Flow (2.5 μg).
- Storage conditions: -20°C, avoid freeze/thaw cycles.
- Shipping conditions: Ambient (domestic); wet ice (international).
Expression Systems S2 DROSOPHILA CELLS ESF 921
Expression Systems offers insect cell lines adapted to serum-free suspension culture in either ESF 921 or ESF AF. The availability of pre-adapted cells saves significant time and expense associated with the adaptation of cultures.
The S2 insect cell line is derived from a primary culture of late stage (20-24 hours old) Drosophila melanogaster embryos. S2 cells are adapted for suspension culture in ESF 921 and are available as a frozen vial or suspension culture.
• Recombinant protein expression from transient expression or stable cell line
• Good growth in adherent or suspension culture
• Quality and performance testing
To Download Instructions for Use and/or COA please visit: www.expressionsystems.com
As One International Inc Green Fluorescent primary HUVEC - tube formation assay cells, 3 x 10^6 cells
Green Fluorescent primary HUVEC - tube formation assay cells, 3 x 10^6 cells
Lonza Walkersville Mouse Embry Fibro Nufe Kit 1
The Nucleofector 2b System (or older generations I or II) works cell type specific kits each of them dedicated to an individual primary cell The Mouse Embryonic Fibroblast Nucleofector Kit 1 is one of our two kits suited for efficient transfection of primary mouse embyonic fibroblasts (MEF) Due to significant diversity between different MEF clones we have developed two Nucleofector Kits (1 and 2) and an easy-to-use Nucleofector Starter Kit to determine which of the 2 kits is optimal for a specific MEF clone The respective cell type-specific Optimized Protocol or recommendation is available under Instructions or in our Lonza Bioscience knowledge database In this Optimized Protocol the best Nucleofection conditions are indicated In addition we share our experience and knowledge for treatment of this cell type
Lonza Walkersville PNBM Primary Neuron Basal Med
Clonetics PNBM Primary Neuron Basal Medium (CC-3256) specifically developed for culturing embryonic primary rat and mouse neurons For successful culture of neurons basal media should be combined with growth factors (sold seperately) Basal medium and SingleQuots Growth Factors can be purchased seperately or in convenient PNGM BulletKit format (CC-4461)
Lonza Walkersville NHCF-A - Hu Atrial Cardiac Fib
Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or ventricle Cells are isolated from normal adult heart tissue Cryopreserved cells are shipped at passage 2 Clonetics NHCF-V -A are guaranteed through 5 population doublings and express 90% collagen I and 10% Von Willibrand factor VIII after thaw Clonetics AoAF are guaranteed through 10 population doublings and are negative for alpha smooth muscle actin All cells test negative for mycoplasma bacteria yeast and fungi HIV-1 hepatitis B and hepatitis C are not detected for all donors and/or cell lots A Certificate of Analysis (CoA) is provided for each cell lot purchased
Lonza Walkersville OBM Osteoblast Growth and Dif
OBM is an osteoblast cell growth basal medium sold separately This can be used with 1 kit of CC-4193 (osteoblast growth medium SingleQuots Supplements and Growth Factors) The full OGM BulletKit consisting of basal medium and supplements can be purchased together using the catalog number CC-3207 Clonetics OGM Osteoblast Growth Medium is specially formulated for the growth and expansion of human osteoblasts The kit contains 10% serum OGM Differentiation Supplements are also included and are recommended for use with differentiation assays The kit is conveniently packaged to include basal medium with growth and differentiation supplements for complete culture of osteoblasts
Lonza Walkersville D-RPTEC - Diabetes Type II
Lonza s Human Renal Cells include cells isolated from various normal human kidney tissue specific to each cell type Human Renal Proximal Tubule Cells are isolated from the proximal tubule and are ideal for drug transporter studies Renal epithelial cells display a polarized morphology which is essential for their function They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate while allowing other substances of no nutritional value to be excreted in the urine Physiological processes like osmoregulation and excretion can be studied with HRE in vitro
Lonza Walkersville Human NK Cells pos select 5M
Natural Killer (NK) cells are lymphocytes of the immune system that are critical in host defense and immune regulation as part of both the innate and adaptive immune response They are of particular interest to immunotherapy researchers as they hold the promise to specifically target and destroy cancer cells and tumors while leaving healthy cells intact Natural Killer cells are traditionally characterized by the presence of the CD56 cell surface marker and absence of CD3 In addition CD16 is important for NK cell function because CD16 induces cytokine expression and cytotoxic effector activity Lonza NK cells are isolated using positive (2W-502) or negative (2W-501) immunomagnetic selection for the CD56 marker Purity is 90% based on the presence of CD56 cells Both positively and negatively selected NK cells are guaranteed to express CD56 and CD16
Lonza Walkersville P5 Prim Cell 96-well Nufe Kit
The P5 Primary Cell 96-well Nucleofector Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector 96-well Unit This kit has been determined to be optimal for transfecting following cell types HUVEC Skeletal Myoblast hum The respective cell type-specific Optimized Protocols or recommendations are available under Instructions or in our Lonza Bioscience knowledge database In these Optimized Protocols the best Nucleofection conditions are indicated In addition we share our experience and knowledge for treatment of individual cell types The kit is available in different variants for either 1 x 96 or 10 x 96 reactions
Cell Biologics C57BL/6 BRAIN ENDOTHELIAL CELL
C57BL/6 MOUSE PRIMARY BRAIN MICROVASCULAR ENDOTHELIAL CELLS - Frozen Vial