DNA Reagents
New England Biolabs, Inc. NEBridge® Golden Gate Enzyme Kit (BsaI-HF®v2) – 20 reactions
The NEBridge Golden Gate Assembly Kit (BsaI-HFv2) contains an optimized mix of BsaI-HFv2 and T4 DNA Ligase. Together these enzymes can direct the assembly of multiple inserts/modules using the Golden Gate approach. Also included is the pGGAselect destination plasmid, which provides a backbone for your assembly, features convenient restriction enzyme sites for subcloning, and has T7/SP6 promoter sequences to enable in vitro transcription. Please note that while general descriptions regarding Golden Gate Assembly use the BsaI nomenclature, this kit and protocols feature the specific engineered form optimized for Golden Gate Assembly, BsaI-HFv2.
- Kit also available with BsmBI-v2
- Seamless cloning - no scar remains following assembly
- Includes destination plasmid with T7/SP6 promoters
- Ordered assembly of multiple fragments (2-20+) in a single reaction
- Can also be used for cloning of single inserts and library preparations
Chemgenes Corporation Small Drying Traps, 10 pack, effective for 10-50 mL acetonitrile solutions, reduces moisture from 250 ppm to below 50 ppm, pre-activated, vacuum sealed
The Small Drying Traps are designed to maintain highly anhydrous environments essential for high-quality oligonucleotide synthesis. These traps effectively dry acetonitrile solutions ranging from 10-50 mL, significantly reducing moisture content from approximately 250 ppm to below 50 ppm. They come pre-activated and are packaged in vacuum-sealed foil packets for immediate usability. These traps are crucial for improving coupling efficiency during DNA/RNA synthesis, making them a vital part of laboratory workflows.
- Creates super anhydrous conditions critical for DNA/RNA synthesis
- Reduces moisture in acetonitrile from 250 ppm to below 50 ppm
- Supplied fully activated for immediate use
- Compatible with various acetonitrile-based solutions
- Enhances coupling yields during oligonucleotide synthesis
- Packaged in vacuum-sealed foil packets
- 10 traps per pack
Invivogen 2'3'-CGAMP
2’3’-cGAMP (cyclic [G(2’,5’)pA(3’,5’)p]) is produced in mammalian cells by cGAS (cGAMP synthase) in response to double-stranded DNA in the cytoplasm. 2’3’-cGAMP is also referred to as “noncanonical“ cGAMP due to the presence of the atypical 2’-5’ phosphodiester linkage between the guanosine and the adenosine. Similar to the canonical 3’3’-cGAMP, 2’3’-cGAMP serves as a second messenger to activate innate immune responses by binding to STING (stimulator of IFN genes) and subsequently inducing the TBK1-IRF3-dependent production of IFN-β [1]. Structural and functional studies revealed that noncanonical 2’3’-cGAMP is distinct from the canonical 3’3’-cGAMP produced by bacteria [2,3]. 2'3'-cGAMP binds to STING with a much greater affinity than 3'3'-cGAMP [1]. Certain variants of STING are able to distinguish between noncanonical and canonical cGAMP [2]
Zymo Research Corporation RRHP 5-hmC Library Prep Kit 25 preps
The RRHP 5-hmC Library Prep Kit is an all-inclusive solution for analysis of genome-wide 5-hydroxymethylcytosine (5-hmC) positions at single-base resolution. The Reduced Representation Hydroxymethylation Profiling (RRHP) method is based on blocking MspI digestion by glucosylating 5-hmC within MspI recognition sites. Fragments lacking glucosylated 5-hmC at the adapter-ligation junction will be cleaved and not amplified by PCR. Therefore, only fragments containing 5-hmC will be successfully amplified and analyzed by Next-Generation Sequencing. Fragments with higher 5-hmC levels will be correlated with higher frequency of sequencing reads. RRHP bypasses the need for bisulfite conversion, which allows for DNA inputs as low as 100 ng, lower sequencing depth, and straight-forward bioinformatics processing.
Hoefer, Inc Hoefer™ SE250 Mighty Small II Mini Vertical Protein Electrophoresis Unit (Basic unit)
Designed for quick screening and rapid separationFeatures:Maximum glass plate size 10 x 8 cmEfficient active cooling ensures sharp bandsQuick and easy to assembleRequires minimal bufferEasily upgradable to the SE260 (with optional accessories) resulting in the additional capability of accommodating both 10 x 8 and 10 x 10.5 cm gels, enabling a wider variety of precast gel optionsComplete Units Include:Buffer Chamber with Cooling Core; Safety Lid with High Voltage Leads; Glass Plates, Rectangular, 10 x 8 cm, 10 pcs; Alumina Plates, Notched, 10 x 8 cm, 2 pcs; Combs,10 well, 2 pcs; T-Spacers, 4 pcs; Gel casterBasic Unit Includes:The same as complete unit without the combs, spacers, and caster.Specifications:Glass Plate Size (W x H) 10 x 8 cmSpacers 0.75 mm, 1.0 mm, 1.5 mmMaximum Power Settings 500 V, 500 mA, 12 WMaximum Temperature 45°CIndoor Use 4 to 40°CHumidity Up to 80%Unit Dimension (W x H x D) 16.5 x 16 x 16 cm
Cayman Chemical EP4 Receptrrat ActIvatIon 1ea
The EP4 receptor is one of four GPCRs that mediate the actions of prostaglandin E2 (PGE2). The diverse effects of PGE2 acting via EP4 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Cayman's EP4 Receptor (rat) Activation Assay Kit (cAMP) consists of a 96-well plate coated with a transfection complex containing a DNA construct for rat EP4 Receptor (EP4 Reverse Transfection Strip Plate). Cells grown on the transfection complex will express EP4 at the cell surface. Binding of agonists to EP4 stimulates cAMP generation and increases intracellular cAMP levels, which can be measured by a competitive EIA using the Mouse Anti-Rabbit IgG Coated Plate and the reagents included in the kit. An EP4 agonist, PGE2, is included in the kit as a positive control.
Syd Lab Inc Anti-HIV V3 Antibody (Clone: PGT121) | PA007172, 25mg
The research grade anti-HIV V3 loop glycan monoclonal antibody (clone PGT121) is for research use only (RUO).