Cell Based Assays
Promega AKT3 Kinase Enzyme System, 1mg
Recombinant full-length human AKT3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT3 or Protein Kinase B gamma (PKBgamma) is a serine/threonine kinase that is a member of the AKT family.
Promega ADP-Glo™ Kinase Assay + ABL1 (Y253F) Kinase Enzyme System
Recombinant human ABL1 (Y253F) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + MLK1 Kinase Enzyme System
Recombinant human mLK1 (1-433) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway.
Promega CDK5/p35 Kinase Enzyme System, 10microg
Recombinant full-length human CDK5 and human p35 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.
Promega HIPK1 Kinase Enzyme System, 1mg
Recombinant human HIPK1 (156-555) was expressed in Sf9 cells using an N-terminal GST tag. HIPK1 or homeodomain-interacting protein kinase 1 is a ser/thr protein kinase and a member of the HIPK family.
Promega TAK1-TAB1 Kinase Enzyme System, 1mg
Recombinant human proteins TAK1 (1-303) and TAB1 (437-end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega MINK1 Kinase Enzyme System, 10microg
Recombinant human MINK1 (1-320) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. MINK1 is a member of the germinal center family of kinases that are homologous to the Ste20 family and regulate a wide variety of cellular processes.
Promega PDGFR alpha (T674I) Kinase Enzyme System, 1mg
Recombinant human PDGFRα (Y674I) (550-end) point mutant was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega SGK1 Kinase Enzyme System, 1mg
Recombinant human SGK1 (amino acids 60-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SGK1 is a member of the serum- and glucocorticoid-induced protein kinase family.
Promega ALK2 Kinase Enzyme System, 1mg
ALK2 is a receptor serine/threonine kinase that is member of the ALK family and is upstream of signaling pathway involving the SMAD proteins especially SMAD1/5/8.
Promega JNK1 Kinase Enzyme System, 10microg
Recombinant full-length human JNK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK1 is a member of the MAP kinase group that is activated by dual phosphorylation at the thr and tyr residues during exposure to stress.
Promega DAPK1 Kinase Enzyme System, 10microg
Recombinant human DAPK1 (1-363) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Death-associated protein kinase 1 (DAPK1) is a positive mediator of apoptosis induced by gamma-interferon.
Promega MLK1 Kinase Enzyme System, 10microg
Recombinant human MLK1 (1-433) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway.
Promega ADP-Glo™ Kinase Assay + p387beta; Kinase Enzyme System
Recombinant full-length human p38 beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38 beta is a member of the p38 MAP kinase family and is activated by both proinflammatory cytokines and environmental stress.
Promega ADP-Glo™ Kinase Assay + EGFR (T790M) Kinase Enzyme System
Recombinant human EGFR (T790M) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.