Cell Based Assays
Promega NanoBiT PPI Flexi Starter System, 1 each
The NanoBiT PPI Starter Systems provide the vectors required to create the LgBiT and SmBiT protein fusions, a PRKACA:PRKAR2A positive control pair and a negative control vector. Starter systems also include the Nano-Glo Live Cell Assay System.
Promega Glucose Uptake-Glo Assay, 10ml
The Glucose Uptake-Glo Assay is a non-radioactive, plate-based, homogeneous bioluminescent method for measuring glucose uptake in mammalian cells based on the detection of 2-deoxyglucose-6-phosphate (2DG6P).
Promega STK33 Kinase Enzyme System, 10microg
Recombinant full-length human STK33 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. STK33 is a distant member of the CAMK group of serine/threonine kinases.
Promega ZAK Kinase Enzyme System, 1mg
Recombinant full-length human ZAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest.
Promega ADP-Glo™ Kinase Assay + ULK1 Kinase Enzyme System
Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ULK1 is a serine/threonine protein kinase that plays a critical role during initial stages of autophagy, a vital response to nutrient starvation.
Promega ADP-Glo™ Kinase Assay + FLT3 (D835Y) Kinase Enzyme System
Recombinant human FLT3 (D835Y) (571-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega EGFR (L858R) Kinase Enzyme System, 10microg
Recombinant human EGFR (L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega AMPK (A1/B1/G2) Kinase Enzyme System, 1mg
Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMP-activated protein kinase (AMPK) exhibits a key role as master regulator of cellular energy homeostasis.
![Promega PI3K (p110alpha[H1047R]/p85alpha), 20ug, 200microl](https://assets.fishersci.com/TFS-Assets/CCG/product-images/PRV1741.jpg-250.jpg)
Promega PI3K (p110alpha[H1047R]/p85alpha), 20ug, 200microl
The PI3K lipid kinase enzymes are used with ADP-Glo KInase Assay System and Lipid Kinase Substrates in a homogeneous, add-mix-read lipid kinase assay.
Promega TBK1 Kinase Enzyme System, 1mg
Recombinant full-length human TBK1 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases.
Promega ADP-Glo™ Kinase Assay + ALK4 Kinase Enzyme System
Recombinant human ALK4 (150-end) was expressed in Sf9 cells using an N-terminal GST tag. ALK4 is a member of the subfamily of receptor ser/thr kinases that mediates signaling by the Activins.
Promega HER2 Kinase Enzyme System, 1mg
Recombinant human HER2 (amino acids 676-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HER2 gene encodes a cell-surface glycoprotein tyrosine kinase receptor with extensive homology to epidermal growth factor receptor.
Promega p70S6K Kinase Enzyme System, 1mg
Recombinant full-length human p70S6K was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues.
Promega CAMKK1 Kinase Enzyme System, 10microg
CAMKK1 or CAMKK alpha is a Ca2+/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively.
Promega CDK5/p35 Kinase Enzyme System, 1mg
Recombinant full-length human CDK5 and human p35 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.