Signal Transduction Reagents and Kits
Promega PYK2 Kinase Enzyme System, 10microg
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
Promega TGF beta R1 Kinase Enzyme System, 10microg
TGF beta R1 is a member of the TGF beta receptor subfamily and is a ser/thr protein kinase that forms a heteromeric complex with type II TGF-beta receptors when bound to TGF-beta, transducing the TGF beta signal from the cell surface to the cytoplasm.
Promega ADP-Glo™ Kinase Assay + DNA-PK Kinase Enzyme System
Native human DNA-Dependent Protein Kinase purified from HeLa cells. Consists of a ∼460kDa catalytic subunit and a heterodimeric DNA-binding subunit (Ku) containing an 85kDa and a 70kDa peptide (1). Gene sequence can be found at accession number NM_006904.
Promega CK1 alpha 1 Kinase Enzyme System, 10microg
Full-length recombinant human CK1 alpha 1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK1 alpha 1 is a member of the CK1 family of serine/threonine protein kinases, which play an important role in diverse cell processes.
Promega ADP-Glo™ Kinase Assay + CDK2/CyclinE1 Kinase Enzyme System
Recombinant full-length human CDK2 and CyclinE1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK2 is a member of the Cyclin-Dependent Kinase family that is ubiquitously expressed.
Promega VRK2 Kinase Enzyme System, 10microg
Recombinant human VRK2 (1-375) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. VRK2 (also known as vaccinia related kinase 2) is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases.
Promega ADP-Glo™ Kinase Assay + ABL1 (Y253F) Kinase Enzyme System
Recombinant human ABL1 (Y253F) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + ABL1 (T315I) Kinase Enzyme System
Recombinant human ABL1 (T315I) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + CLK3 Kinase Enzyme System
Recombinant full-length human CLK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CLK3, also known as CDC-like kinase 3, encodes a serine/threonine-type protein kinase with a non-conserved N-terminal domain.
Promega ADP-Glo™ Kinase Assay + ZAK Kinase Enzyme System
Recombinant full-length human ZAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest.
Promega ADP-Glo™ Kinase Assay + CAMKK1 Kinase Enzyme System
CAMKK1 or CAMKK alpha is a Ca2+/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively.
Promega MLK2 Kinase Enzyme System, 10microg
Recombinant human MLK2 (1-446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8/JNK1 and MKK4/SEK1.
Promega ADP-Glo™ Kinase Assay + EGFR (L858R) Kinase Enzyme System
Recombinant human EGFR (L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is the receptor for members of the EGF family and is a transmembrane glycoprotein that has tyrosine kinase activity.
Promega IKK beta Kinase Enzyme System, 10microg
Full length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + WNK1 Kinase Enzyme System
Recombinant human WNK1 (181-507) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.