Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + IKKα Kinase Enzyme System
Full-length recombinant human IKK alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKK alpha is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein.
Promega ADP-Glo™ Kinase Assay + BRK Kinase Enzyme System
Recombinant full-length human BRK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. BRK is a member of the non-receptor tyrosine kinases (PTKs) that contains an amino terminal SH3 and SH2 domain as well as the catalytic domain.
Promega ADP-Glo™ Kinase Assay + p38δ Kinase Enzyme System
Recombinant full-length human p38 delta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. It is a member of the p38 MAPK family and is activated by chemical and environmental stresses as well as by proinflammatory cytokines.
Promega ADP-Glo™ Kinase Assay + HIPK1 Kinase Enzyme System
Recombinant human HIPK1 (156-555) was expressed in Sf9 cells using an N-terminal GST tag. HIPK1 or homeodomain-interacting protein kinase 1 is a ser/thr protein kinase and a member of the HIPK family.
Promega ADP-Glo™ Kinase Assay + HPK1 Kinase Enzyme System
Recombinant human HPK1 (1-346) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HPK1 or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family.
Promega ADP-Glo™ Kinase Assay + RIPK2 Kinase Enzyme System
RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein that contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain.
Promega ADP-Glo™ Kinase Assay + CHK2 Kinase Enzyme System
Recombinant full-length human CHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage.
Promega ADP-Glo™ Kinase Assay + TAK1-TAB1 Kinase Enzyme System
Recombinant human proteins TAK1 (1-303) and TAB1 (437-end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega CHK2 Kinase Enzyme System, 10microg
Recombinant full-length human CHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage.
Promega STK33 Kinase Enzyme System, 10microg
Recombinant full-length human STK33 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. STK33 is a distant member of the CAMK group of serine/threonine kinases.
Promega ADP-Glo™ Kinase Assay + PYK2 Kinase Enzyme System
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
Promega ADP-Glo™ Kinase Assay + HIPK3 Kinase Enzyme System
Recombinant human HIPK3 (163-562) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. HIPK3 (homeodomain interacting protein kinase 3) is a member of the HIPK family and is involved in apoptosis.
Promega MET (M1250T) Kinase Enzyme System, 10microg
Recombinant human MET (point mutant M1250T; amino acids 956-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega MAPKAPK5 Kinase Enzyme System, 10microg
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
Promega ADP-Glo™ Kinase Assay + MAPKAPK5 Kinase Enzyme System
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.