Enzymes and Inhibitors
GENOVIS INC FabRICATOR LE, 5000 Units
FabRICATOR (IdeS) is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F(ab')2 and Fc/2 fragments. No other substrate besides IgG is known.The IgG is digested within 30 minutes, and there is no risk of overdigestion if the incubation time is prolonged. Since FabRICATOR digests IgG under physiological reaction conditions, the immunoreactivity is preserved. FabRICATOR digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG. It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species, we recommend using FabRICATOR Z .
New England Biolabs, Inc. EnGen Sau Cas9 – 400 pmol
EnGen Sau Cas9 from Staphylococcus aureus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA. Targeting requires a ~100 nucleotide guide RNA (gRNA) complementary to the target site, as well as a 5' NNGRRT 3 (N = any, R = A or G) protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. DNA cleavage by EnGen Sau Cas9 occurs ~3 bases 5' of the PAM. EnGen Sau Cas9 has Simian virus 40 (SV40) T antigen nuclear localization signals (NLS) at both the N- and C-termini of the protein. Schematic representation of Sau Cas9 nuclease sequence recognition and DNA cleavage
Worthington Biochemical Corporation DEOXYRIBONUCLEASE 1
Recombinant Bovine pancreatic deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases & proteases. A liquid preparation in 5mM Calcium Acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.
Enzo Life Sciences MMP-8 (catalytic domain) (human), (recombinant) (10 µg)
Alternative name: Matrix metalloproteinase 8, Neutrophil collagenase, Collagenase 2. Purity: ≥90% (SDS-PAGE). Formulation: Liquid. In 50mM TRIS, 5mM CaCl2, 300mM NaCl, 20µM ZnCl2, 0.5% Brij-35, and 30% glycerol. Source: Produced in E. coli. Active Matrix Metalloproteinase-8 (MMP-8, neutrophil collagenase, collagenase-2) catalytic domain from human cDNA. The enzyme consists of the catalytic domain of human MMP-8 (Phe99-Gln269, NM_002424) with a C-terminal purification tag. This represents a naturally-occurring active form of MMP-8 which lacks the C-terminal hemopexin domain1. MMPs lacking this domain cannot cleave native collagens; however, activity toward other targets such as gelatin, casein, or peptide substrates is unaffected. Long Term Storage: -80°C.
New England Biolabs, Inc. EnGen Spy Cas9 HF1 - 2500 pmol
EnGen Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen Spy Cas9 NLS from Streptococcus pyogenes with reduced non-specific DNA cleavage. Spy Cas9 is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The single guide RNA (sgRNA) targets Cas9 to the region immediately upstream of a 5'-NGG-3' protospacer adjacent motif (PAM) producing a double stranded break 3 bases upstream of the PAM. EnGen Spy Cas9 HF1 contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C-termini of the protein.
ACROBiosystems GENIUS™Nuclease DMF Filed
GENIUS™Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli cells using a proprietary process at ACRObiosystems. GENIUS™Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form ( single stranded, double stranded, linear, circular and supercoiled ). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5‘- phosphorylated oligonucleotides of 3-8 bases in length.
Apexbio Technology LLC Dyngo-4a 10mg. 1620401-82-2. MFCD28167817
Dyngo-4a 10mg. 1620401-82-2. MFCD28167817
AG Scientific Inc Pyruvate Oxidase 1 KU
Pyruvate oxidase is originated from Aerococcus viridans and used as a component of screen-printed phosphate biosensor for monitoring phosphate concentrations in a sequencing batch reactor (SBR) system. It consumes phosphate in the presence of pyruvate and oxygen and generates hydrogen peroxide, carbon dioxide and acetylphosphate. The enzyme is a flavoprotein with the relatively tightly bound cofactors flavin adenine dinucleotide, thiamine pyrophosphate, and a divalent metal ion, with Mn2+ being the most effective.Activity: 40 units/mg powderOne unit is defined as amount of enzyme required to generate 1 μmol of H2O2 per minute at 37°C, pH 6.5.
Cayman Chemical Coenzyme A sodium salt hydra
An essential cofactor functioning as an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism; the pantothenate kinase step of the coenzyme A biosynthetic pathway has been identified as a target for the development of antibacterial compounds
Cayman Chemical Coenzyme A sodium salt hydra
An essential cofactor functioning as an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism; the pantothenate kinase step of the coenzyme A biosynthetic pathway has been identified as a target for the development of antibacterial compounds
Cayman Chemical Coenzyme A sodium salt hydra
An essential cofactor functioning as an acyl group carrier and carbonyl-activating group for the citric acid cycle and fatty acid metabolism; the pantothenate kinase step of the coenzyme A biosynthetic pathway has been identified as a target for the development of antibacterial compounds
Diagenode Inc Tagmentase TN5 TRN Unloaded, 10 μl, Hyperactive Tn5 transposase, Mg++ dependent, 19-bp ME DNA sequence required, 2 mg/ml protein concentration, 53.3 kDa molecular weight
Diagenode Tagmentase (Tn5 transposase) is a hyperactive enzyme designed for a wide range of molecular biology applications. It enables an efficient "cut and paste" tagmentation reaction, allowing simultaneous DNA cleavage and the insertion of specific oligonucleotides or sequencing adapters. As an unloaded variant, it provides researchers with the flexibility to customize the enzyme with oligonucleotides tailored to their specific experimental needs. This Tn5 transposase is essential for various Next-Generation Sequencing workflows.
- Enzyme type: Hyperactive Tn5 transposase
- Loading status: Unloaded
- Function: Catalyzes "cut and paste" tagmentation
- Activity requirement: Mg++ dependent
- Optimal pH: 7.5-8.0
- Optimal temperature: 37-55 °C
- Storage conditions: Store at -20 °C
- Protein concentration: 2 mg/ml
- Volume: 10 μl
Bioworld Lysozyme, 25 g
Lysozyme Enzyme used in purification protocols of genomic and plasmid DNA to lyse bacterial cells.Ultra Pure GradeSource: Hen Egg White (also called Hen Egg Lysozyme or HEL.)Activity(25 C): >20,000U/mgAlbumin: None detectedSalmonella: None detected