Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.

Search Within Results

961 – 975 of 5,382 results

961

Promega cAMP-Dependent Protein Kinase, Catalytic Subunit

May be used to phosphorylate target proteins or for in vitro enzymological studies of neural and hormonal signal transduction

Pricing & Availability
Specifications

Product Type	cAMP-dependent Protein Kinase
Concentration	1.5 to 3 mg/mL

962

Components	10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Ribonuclease
Storage Buffer	10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Molecular Weight (g/mol)	27
Color	Colorless
pH	7.3
Concentration	5 to 10 U/μL
For Use With (Application)	Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Source	Recombinant E. coli strain

Promega Alkaline Phosphatase, Calf Intestinal

Alkaline Phosphatase catalyzes the hydrolysis of 5'-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Alkaline Phosphatase
Storage Buffer	10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Color	Colorless
pH	8
Quantity	1000 U
For Use With (Application)	Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Source	Calf intestinal mucosa

964

Thermo Scientific™ CHKtide

A peptide substrate based upon CDC25C that is phosphorylated by CHEK1 and other kinases.

Pricing & Availability

965

pH	10
Storage Requirements	4°C

Promega Restriction Enzymes - Sma I Blue/White Cloning and Genome Qualified (J)

XmaI is a neoschizomer of SmaI. Available at high concentration, containing 5,000 units of SmaI at a concentration of 40-80u/μL. (CCC/GGG GGG\CCC)

Pricing & Availability
Specifications

Cut Site	CCC.GGG GGG.CCC
Form	Liquid
Storage Buffer	10mM Tris HCl (pH 7.4), 300mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	Provides a higher level of quality control for Enzymes used in cloning applications

967

Promega Restriction Enzymes - Rsa I (C)

Capable of digesting DNA in 15 minutes or less. Available at high concentration, containing 5,000 units of RsaI at a concentration of 40-80u/μL. (GT/AC CA\TG)

Pricing & Availability
Specifications

Cut Site	GT.AC CA.TG
Content And Storage	-30°C to -10°C
Form	Liquid
Storage Buffer	10mM Tris HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	Capable of digesting DNA in <15 min.

968

Promega Restriction Enzymes - Xba I Blue/White Cloning and Genome Qualified (D)

Capable of digesting DNA in 15 minutes or less. Genome qualified to ensure optimal performance in genomic analysis applications. (T/CTAG A A GATC\T)

Pricing & Availability
Specifications

Cut Site	T.CTAGA AGATC.T
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	XbaI
Storage Buffer	10mM Tris HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
Concentration	8 to 12 U/μL
For Use With (Application)	Provides a higher level of quality control for Enzymes used in cloning applications

969

Promega Restriction Enzymes - BamH I Blue/White Cloning Qualified (E)

Capable of digesting DNA in 15 minutes or less. Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications. (G/GATC C C CTAG\G)

Pricing & Availability
Specifications

Cut Site	G.GATCC CCTAG.G
Form	Liquid
Storage Buffer	10mM Tris HCl (pH 7.4), 300mM KCl, 5mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/mL BSA, 50% Glycerol
pH	7.4
Incubator Temperature	37°C
For Use With (Application)	Provides a higher level of quality control for Enzymes used in cloning applications

970

Promega AttoPhos™ AP Fluorescent Substrate

A highly sensitive, flluorescent alkaline phosphatase substrate.

Pricing & Availability
Specifications

Form	Powder
Substrate Type	AttoPhos Substrate BBT
Storage Requirements	4°C

971

Promega Glu-C Sequencing Grade Serine Protease

Glu-C, Sequencing Grade (S. aureus V8), is a serine protease that specifically cleaves at the C-terminus of either aspartic or glutamic acid residues. Glu-C activity is optimal in the pH range of 4.0-9.0 and suitable for digestion reactions in solution.

Pricing & Availability

972

Promega Restriction Enzymes - Nde I (D)

Active and capable of digesting DNA in 15 minutes or less, directly in GoTaq™ Green Master Mix. (CA/TA TG GT AT\AC)

Pricing & Availability

973

Promega Pepsin

Pepsin preferentially cleaves at the C-terminus of phenylalanine, leucine, tyrosine and tryptophan. Pepsin can be used alone or with other proteases for protein analysis by mass spectrometry and other applications. Activity is optimal at pH 1.0-3.0.

Pricing & Availability

974

Promega Terminal Deoxynucleotidyl Transferase

TdT catalyzes the addition of mononucleotides to the terminal 3'-OH of a DNA initiator accompanied by the release of inorganic phosphate.

Pricing & Availability
Specifications

Components	5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Terminal Deoxynucleotidyl Transferase
Storage Buffer	50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol
Color	Colorless
pH	6.4
Concentration	30 U/μL
For Use With (Application)	Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays
Source	Recombinant E. coli strain

975

Promega T4 DNA Ligase Buffer Pack

For ligation of DNA ends.

Pricing & Availability

Enzymes and Inhibitors

Promega cAMP-Dependent Protein Kinase, Catalytic Subunit

Promega RNase One™

Promega Alkaline Phosphatase, Calf Intestinal

Thermo Scientific™ CHKtide

Promega AttoPhos™ Buffer

Promega Restriction Enzymes - Sma I Blue/White Cloning and Genome Qualified (J)

Promega Restriction Enzymes - Rsa I (C)

Promega Restriction Enzymes - Xba I Blue/White Cloning and Genome Qualified (D)

Promega Restriction Enzymes - BamH I Blue/White Cloning Qualified (E)

Promega AttoPhos™ AP Fluorescent Substrate

Promega Glu-C Sequencing Grade Serine Protease

Promega Restriction Enzymes - Nde I (D)

Promega Pepsin

Promega Terminal Deoxynucleotidyl Transferase

Promega T4 DNA Ligase Buffer Pack

Enzymes and Inhibitors

Filtered Search Results

Narrow Results