Modifying Enzymes

Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.

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316 – 330 of 2,693 results

316

Promega Sequencing Grade Modified Trypsin, Frozen

Sequencing Grade Trypsin has been manufactured to provide maximum specificity. Lysine residues in porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion.

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317

Promega Exonuclease III

Exonuclease III is a 3' → 5' exonuclease specific for dsDNA.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
Content And Storage	-30°C to -10°C
Inhibitors	50mM NaCl
Form	Liquid
Product Type	Exonuclease III
Storage Buffer	20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Molecular Weight (g/mol)	28000
Color	Colorless
pH	7.5
Purity	>90%
Concentration	150 to 200 U/μL
For Use With (Application)	Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Source	Recombinant E. coli strain

318

Promega Alkaline Phosphatase, Calf Intestinal

Alkaline Phosphatase catalyzes the hydrolysis of 5'-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Alkaline Phosphatase
Storage Buffer	10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Color	Colorless
pH	8
Quantity	1000 U
For Use With (Application)	Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Source	Calf intestinal mucosa

319

Promega cAMP-Dependent Protein Kinase, Catalytic Subunit

May be used to phosphorylate target proteins or for in vitro enzymological studies of neural and hormonal signal transduction

Pricing & Availability
Specifications

Product Type	cAMP-dependent Protein Kinase
Concentration	1.5 to 3 mg/mL

320

Promega Arg-C Sequencing Grade Modifying Enzyme

Arg-C (clostripain) is an endopeptidase that cleaves at the C-terminus of arginine residues, including the sites next to proline. Cleavage also will occur at lysine residues. Arg-C activity is optimal in the pH range of 7.6-7.9.

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321

Promega Asp-N Sequencing Grade Endoproteinase

Asp-N, Sequencing Grade, is an endoproteinase that hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues: Asp and Cys.

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322

Thermo Scientific™ T7 Gene 6 Exonuclease

Degrades nucleotides at the gaps and nicks of double-stranded DNA from the 5'-termini and RNA from RNA:DNA hybrids in the 5'→3' direction

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323

Promega Elastase

Elastase is a serine protease that preferentially cleaves at the C-terminus of alanine, valine, serine, glycine, leucine or isoleucine. Elastase has a unique capability of digesting elastin. Elastase activity is optimal at pH 9.0.

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324

Promega RNase One™

Single-strand-specific RNA endonuclease. Cleaves between all ribonucleotides.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Ribonuclease
Storage Buffer	10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Molecular Weight (g/mol)	27
Color	Colorless
pH	7.3
Concentration	5 to 10 U/μL
For Use With (Application)	Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Source	Recombinant E. coli strain

325

Promega Klenow Fragment, DNA Polymerase I

A DNA-dependent DNA polymerase that lacks the 5'→3' exonuclease activity of intact E. coli DNA Polymerase I but retains its 5'→3' polymerase, 3'→5' exonuclease and strand displacement activities.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, 1mM DTT
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	DNA Polymerase I Large (Klenow) Fragment
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Molecular Weight (g/mol)	68
Color	Colorless
pH	7.2
Purity	>80%
For Use With (Application)	Filling-in or labeling 3' ends of 5' overhanging double-stranded DNA, second-strand cDNA synthesis, DNA sequencing by the Dideoxy method
Source	Recombinant E. coli strain

326

Promega T4 Polynucleotide Kinase Buffer Pack

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.

Pricing & Availability

327

Promega DNA Polymerase I

DNA Polymerase I catalyzes the template-directed polymerization of nucleotides into duplex DNA in a 5'→3' direction. Recombinant.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 500mM Tris HCl (pH 7.2 at 25°C), 100mM MgSO4, DTT 1mM
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	DNA Polymerase I
Storage Buffer	50mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 0.1mM EDTA, 50% Glycerol
Color	Colorless
pH	7.5
Purity	>90%
Concentration	5 to 10 U/μL
For Use With (Application)	Labeling of DNA to high specific radioactivity by nick translation, second-strand cDNA synthesis
Source	Recombinant E. coli strain

328

Promega SP6 RNA Polymerase

DNA-dependent RNA polymerase for in vitro transcription.

Pricing & Availability
Specifications

Components	100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	SP6 RNA Polymerase
Storage Buffer	20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Color	Colorless
pH	7.5
Purity	>90%
For Use With (Application)	Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Source	Recombinant E. coli strain

329

Promega T4 RNA Ligase

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA.

Pricing & Availability

330

Promega Ribonuclease H

RNase H is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA.

Pricing & Availability
Specifications

Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Ribonuclease H
Storage Buffer	20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 0.2mg/mL BSA, 50% Glycerol
Color	Colorless
pH	7.8
Concentration	0.5 to 2 U/μL
For Use With (Application)	Removal of the RNA strand prior to second strand cDNA synthesis, analysis of in-vitro polyadenylation reaction products, microarrays
Source	Recombinant E. coli strain

Modifying Enzymes

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