Modifying Enzymes

Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.

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346 – 360 of 2,660 results

346

Promega Exonuclease III

Exonuclease III is a 3' → 5' exonuclease specific for dsDNA.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 660mM Tris HCl (pH 8 at 25°C), 6.6mM MgCl2
Content And Storage	-30°C to -10°C
Inhibitors	50mM NaCl
Form	Liquid
Product Type	Exonuclease III
Storage Buffer	20mM Tris HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% Glycerol
Molecular Weight (g/mol)	28000
Color	Colorless
pH	7.5
Purity	>90%
Concentration	150 to 200 U/μL
For Use With (Application)	Generating nested sets of deletions with double-stranded, linear DNA, preparation of single-stranded DNA
Source	Recombinant E. coli strain

347

Promega Asp-N Sequencing Grade Endoproteinase

Asp-N, Sequencing Grade, is an endoproteinase that hydrolyzes peptide bonds on the N-terminal side of aspartic and cysteic acid residues: Asp and Cys.

Pricing & Availability

348

Promega RNase One™

Single-strand-specific RNA endonuclease. Cleaves between all ribonucleotides.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: Tris HCl 100mM (pH 7.5 at 25°C), EDTA 50mM, Sodium Acetate 2M
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Ribonuclease
Storage Buffer	10mM Tris HCl (pH 8), 200mM NaCl, 50% Glycerol
Molecular Weight (g/mol)	27
Color	Colorless
pH	7.3
Concentration	5 to 10 U/μL
For Use With (Application)	Mapping, RNase protection assay, mismatch detection, selective removal of RNA from DNA preparations
Source	Recombinant E. coli strain

349

Promega Alkaline Phosphatase, Calf Intestinal

Alkaline Phosphatase catalyzes the hydrolysis of 5'-phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 0.5M Tris HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM Spermidine
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Alkaline Phosphatase
Storage Buffer	10mM Tris HCl (pH 8), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% Glycerol
Color	Colorless
pH	8
Quantity	1000 U
For Use With (Application)	Prevent recircularization and religation of linearized cloning vector DNA by removing Phosphate groups from both 5'-termini, dephosphorylation of 5' Phosphorylated ends of DNA or RNA for subsequent labeling with (32P)ATP and T4 Polynucleotide Kinase
Source	Calf intestinal mucosa

350

Fisher Science Education™ Lipase Powder

Triacylglycerol Hydrolase; CAS: 9001-62-1

Pricing & Availability
Specifications

Form	Powder
Product Type	Lipase
Color	Beige
Purity	>99%
For Use With (Application)	Laboratory chemicals

351

Form	Solid
Product Type	Trypsin
Color	White
Purity	100%
For Use With (Application)	Laboratory chemicals

352

Fisher Science Education™ Lysozyme

Pricing & Availability

353

Fisher Science Education™ Rennin

Pricing & Availability

354

Edvotek™ Proteinase K

Required to prepare the lysis solution for isolation of DNA from hair.

Pricing & Availability

355

G-Biosciences T4 DNA Ligase

T4 DNA Ligase is used for cloning of restriction enzyme generated DNA fragments and PCR products. It is also used for next-generation library preparation, joining linkers and adapters to cohesive or blunt-ended DNA, self-circularization of linear DNA.

Pricing & Availability
Specifications

Sensitive to Heat Inactivation	Yes
Form	Liquid
Product Type	DNA Ligase
Enzyme	DNA Ligase
Research Category	Genome Editing,Traditional Cloning,Scarless Cloning,Next-generation Sequencing,DNA Repair

356

G-Biosciences Taq DNA Ligase

Taq DNA Ligase uses NAD+ as a cofactor to catalyze the formation of a phosphodiester bond in duplex DNA containing adjacent 5'-phosphoryl and 3'-hydroxyl termini.

Pricing & Availability
Specifications

Sensitive to Heat Inactivation	No
Form	Liquid
Product Type	Taq DNA Ligase
Enzyme	DNA Ligase
Packaging	Vial
Research Category	Traditional Cloning
Product Line	Invitrogen

357

Terminal deoxynucleotidyl transferase (TdT), Murine, 2500 U, G-Biosciences

Terminal Transferase catalyzes the untemplated addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.

Pricing & Availability

358

End-Repair dA-tailing Mix, 24 reactions, G-Biosciences

The End-Repair dA-tailing Mix converts damaged or incompatible 5'/3′ ends to 3' A tailed and 5′-phosphorylated DNA.

Pricing & Availability

359

G-Biosciences T4 UvsX DNA Recombinase

T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes.

Pricing & Availability
Specifications

Sensitive to Heat Inactivation	Yes
Form	Liquid
Product Type	DNA Recombinase

360

T7 Exonuclease, 2500 U, G-Biosciences

Gene 6 protein of bacteriophage T7 is a dsDNA 5' to 3' exonuclease which also exhibits flap endonuclease activity.

Pricing & Availability

Modifying Enzymes

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Fisher Science Education™ Lipase Powder Science EducationA science education product.

Fisher Science Education™ Trypsin Science EducationA science education product.

Fisher Science Education™ Lysozyme Science EducationA science education product.

Fisher Science Education™ Rennin Science EducationA science education product.

Edvotek™ Proteinase K Small and Specialty Supplier PartnerSmall and/or specialty supplier based on Federal laws and SBA requirements.Learn More Science EducationA science education product.

G-Biosciences T4 DNA Ligase Science EducationA science education product.

G-Biosciences Taq DNA Ligase Science EducationA science education product.

Terminal deoxynucleotidyl transferase (TdT), Murine, 2500 U, G-Biosciences Science EducationA science education product.

End-Repair dA-tailing Mix, 24 reactions, G-Biosciences Science EducationA science education product.

G-Biosciences T4 UvsX DNA Recombinase Science EducationA science education product.

T7 Exonuclease, 2500 U, G-Biosciences Science EducationA science education product.