Modifying Enzymes
INTACT GENOMICS INC Taq DNA Ligase 10,000 Units
Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them; therefore, a single-base substitution can be detected. This product is active at elevated temperatures (45°C-70°C) (1, 2).Applications Allele-specific gene detection by using Ligase Detection Reaction (LDR) and Ligase Chain Reaction (LCR) (1). Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification(3).
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa, 9001-78-9, MFCD00131849, 500 mg
Lyophilized powder, ≥10 DEA units/mg solid, storage temp: −20°C, Synonym: Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum).
Haematologic Technologies Inc HUMAN PLASMIN 500UG
Coagulation protein, Human Plasmin, 500 micrograms, Native enzyme, Mode of action: Enzyme involved in fibrinolysis, Research use only, 50% (v/v) glycerol/water, store at -20 Celsius.
Carnabio Usa Inc RSK4(RPS6KA6)/5UG/FULL-LENGTH
RSK4(RPS6KA6), Full-length, Wild type, Amino Acid 1-745, NP_055311.1, Expressed in Insect (sf21); 5microg
Carnabio Usa Inc PDHK4(PDK4)/10UG/FULL-LENGTH
PDHK4(PDK4), Full-length, Wild type, Amino Acid 1-411, NP_002603.1, Expressed in E. coli; 10microg
Carnabio Usa Inc QIK(SNF1LK2)/10UG/FULL-LENGTH
QIK(SNF1LK2), Full-length, Wild type, Amino Acid 1-926, NP_056006.1, Expressed in Insect (sf21); 10microg
Carnabio Usa Inc ROCK1/10UG/CATALYTIC
ROCK1, Catalytic domain, Wild type, Amino Acid 1-477, NP_005397.1, Expressed in Insect (sf21); 10microg
Qiagen Beverly Inc T4 DNA LIGASE (RAPID)
DNA Ligase; T4 DNA Ligase (Rapid); Conc. 600,000 U/ml; 240,000U; incl. 10X buffer and 2X rapid ligation buffer