Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Salt Active Nuclease, 5 KU
Salt active nuclease has been used to remove DNA during protein expression and purification.
Medchemexpress LLC Amylase | 9000-92-4 | 1 G
Amylase is an enzyme produced by the pancreas and salivary glands that catalyzes the hydrolysis of starch into sugars. Amylase is broadly classified into α, β, and γ subtypes. It is for research use only. A mixture of glycosylated amylase and proline-rich proteins or fractions containing mostly non-glycosylated amylase predominantly promotes adhesion of *S. gordonii G9B*. Fractions containing amylase do not promote adhesion of *S. sanguis 10556* to HAP.
- Catalyzes the hydrolysis of starch into sugars
- Available in α, β, and γ subtypes
- Suitable for research applications
- Offered in various quantities
- Includes data sheet, SDS, COA, and handling instructions
- Appears as a solid, with a color ranging from light yellow to khaki
- Can be sourced from plants, animals, and microorganisms
ABclonal Technology T4 DNA Ligase (High Conc.)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline from bovine intestinal mucosa BioUltra, buffered aqueous glycerol solution, ≥6,000 DEA units/mg protein | 9001-78-9 | MFCD00131849 | 10ku
Phosphatase, Alkaline from bovine intestinal mucosa BioUltra, buffered aqueous glycerol solution, ≥6,000 DEA units/mg proteinMW: | 9001-78-9 | MFCD00131849 | 10ku
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirachium album 3.0 unit/mg solid, lyophilized powder | 39450-01-6 | MFCD00132129 | 1g
Proteinase K from Tritirachium album 3.0 unit/mg solid, lyophilized powder | 39450-01-6 | MFCD00132129 | 1g
Sigma Aldrich Fine Chemicals Biosciences Lipoxidase from Glycine max (soybean) Type I-B, lyophilized powder, >=50,000 units/mg solid | 9029-60-1 | MFCD00131527 | 15MU
Lipoxidase from Glycine max (soybean) Type I-B, lyophilized powder, >=50,000 units/mg solid | 9029-60-1 | MFCD00131527 | 15MU
Sigma Aldrich Fine Chemicals Biosciences Neuraminidase from Clostridium Perfringens (C. welchii) | 9001-67-6 | MFCD00131711 | 5un
Neuraminidase from Clostridium Perfringens (C. welchii) | 9001-67-6 | MFCD00131711 | 5un
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I RN 100UN
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter (approximately 1 mg/ml) containing 50% glycerol and 1 mM calcium chloride. 9003-98-9
New England Biolabs, Inc. T7 DNA Ligase – 750000 units
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It will catalyze the formation of a phosphodiester bond between adjacent 5' phosphate and 3' hydroxyl groups of duplex DNA. Cohesive end ligation and nick sealing can be efficiently catalyzed by T7 DNA Ligase. However, unlike T4 and T3 DNA Ligases, blunt end ligation is not efficiently catalyzed by T7 DNA Ligase. Addition of high concentrations of PEG 6000 [>= 20% (w/v)] to the reaction can force T7 DNA Ligase to have measurable activity. However, under typical reaction conditions blunt-end DNA ligation does not occur in the presence of T7 DNA Ligase, making it a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined. T7 DNA Ligase does not ligate blunt-end DNA fragments.
Sigma Aldrich Fine Chemicals Biosciences Phosphoglucose Isomerase from rabbit muscle Type XI, lyophilized powder, >=200 units/mg protein | 9001-41-6 | MFCD00131887 | 1KU
Phosphoglucose Isomerase from rabbit muscle Type XI, lyophilized powder, >=200 units/mg protein | 9001-41-6 | MFCD00131887 | 1KU
Discovery Life Sciences HUMAN P450 OXIDOREDUCTASE+B5
BD Gentest, Human P450 Oxidoreductase + b5 Insect Control, Regulatory Status is RUO
ABclonal Technology Circle Ligase
Circle Ligase is a thermostable ATP-dependent ligase that can catalyze the self-circularization of single-stranded DNA (ssDNA)/single-stranded RNA (ssRNA) strands simultaneously possessing a 5-phosphate group and a 3-hydroxyl group in the absence of complementary sequences, with the length of single-stranded DNA (ssDNA)/single-stranded RNA (ssRNA) being greater than 15bp.
Abcam Adenosine 5'-triphosphate (ATP) disodium salt trihydrate, nucleotide coenzyme, 25G
MW 605.24 g/mol, Purity >99%. Central component of energy storage and metabolism in vivo. ATP is used in many cellular processes, respiration, biosynthetic reactions, motility, and cell division. ATP is a substrate of many kinases involved in cell signaling and of adenylate cyclase(s) that produce the second messenger cAMP. ATP provides the metabolic energy to drive metabolic pumps, and serves as a coenzyme in a wide array of enzymatic reactions. P₂ purinergic agonist that increases Ca²+-activated K+ channel activity.
The product is subject to the following: Abcam Restricted Use Statement