Modifying Enzymes
Enzyme Research Laboratories Inc Human a-Thrombin (Factor IIa),1000 U
Please use this part # if ordering a quantity of 10 or more. If less than qty 1-9 please use Fisher part # NC9872266.
Human a-Thrombin (Factor IIa),1000 U,Buffer Composition = 50 mM Sodium Citrate,0.2 M NaCl,0.1% PEG-8000,pH 6.5,Extinction Coefficient (1%) = 18.3,Molecular Weight = 37,000 daltons,Prepared from human Prothrombin by activation with Factor Xa
New England Biolabs, Inc. Exonuclease III (E.coli) – 5000 units
Catalyzes the stepwise removal of mononucleotides from 3'-hydroxyl termini of duplex DNA. A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules. The preferred substrates are blunt or recessed 3'-termini, although the enzyme also acts at nicks in duplex DNA to produce single-strand gaps. 3'-protruding termini are resistant to cleavage; the degree of resistance depends on the length of the extension,with extensions 4 bases or longer being essentially resistant to cleavage. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C>A=T>G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications. Exonuclease III has also been reported to have RNase H, 3'-phosphatase and AP-endonuclease activities.
Zymo Research Corporation 20X SAM (S-adenosylmethionine) (200 μl)
S-adenosylmethionine (SAM) is provided at a 12 mM concentration and is included in the CpG Methylase (M.SssI) set (E2010/E2011).
New England Biolabs, Inc. Antarctic Thermolabile UDG – 100 units
Antarctic Thermolabile UDG (Uracil- DNA Glycosylase) catalyzes the release of free uracil from uracil-containing single-stranded or double-stranded DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperature and high pH. This enzyme is sensitive to heat and can be rapidly and completely inactivated at temperatures above 50C.
New England Biolabs, Inc. PNGase F, Recombinant – 75000 units
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F, Recombinant is a recombinant amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
New England Biolabs, Inc. Bacteroides Heparinase III – 35 units
Bacteroides Heparinase III, also called Heparin Lyase III, is active on both heparin and heparan sulfate. Bacteroides Heparinase III can cleave the glycosidic bond between hexosamines and either iduronic acid or glucuronic acid residues. It is active in the presence of 6-sulfation. The reaction yields oligosaccharide products containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm
Active Motif Preindex Tn5 Transposomes 1x96
Pre-indexed Assembled Tn5 Transposomes are provided in a flexible breakaway 96-well plate format to enable higher throughput ATAC-Seq assays. Each well contains a transposome loaded with a unique i5 and i7 index, enabling direct indexing during tagmentation. By having a unique i7 & i5 Indexed Primer combination in each well, it is easy to perform multiple ATAC-Seq reactions without having to pipette primers separately and keep track of the combinations.
Oxyrase Oxyfluor 5Ml Bottle 1/Pk
Formulated specifically for fluorescence microscopy. Prevents photo-bleaching and photo-damage by removing dissolved oxygen. Significantly reduces/eliminates formation of oxygen free radicals. Results in brighter cell images. Provides protective effect for biological material being studied. Excellent solution for live cell imaging and cell culture applications.
New England Biolabs, Inc. Taq DNA Ligase Reaction Buffer – 6 ml
This is the NEB reaction buffer supplied with Taq DNA Ligase. Taq DNA Ligase requires NAD+ as a cofactor which is incorporated into the buffer. New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
New England Biolabs, Inc. Fpg – 2500 units
Fpg (formamidopyrimidine [fapy]-DNA glycosylase) (also known as 8-oxoguanine DNA glycosylase) acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves both 3' and 5' to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
New England Biolabs, Inc. Enterokinase, light chain – 480 units
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
New England Biolabs, Inc. Mung Bean Nuclease – 1500 units
Mung Bean Nuclease is a single-strand specific DNA and RNA endonuclease which will degrade single-stranded extensions leaving blunt, ligatable ends.