The Monarch Spin gDNA Extraction Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, bacteria and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Monarch products use significantly less plastic in columns and kits compared to leading suppliers. Protocols are also included to enable purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA. Purified gDNA has high quality metrics, including A260/A280 >1.8 and A260/A230 >2.0, high DIN scores and minimal residual RNA. Purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has a peak size of >50kb, making this kit an excellent choice upstream of long-read sequencing platforms