Miscellaneous Molecular Biology Reagents
Agilent Technologies IPTG
IPTG is used in combination with X-gal to detect beta-galactosidase activity with a white/blue color assay and also for screening of transformed colonies.
101 BIO Lentivirus 10X Titer-Up Reagent (Increase virus titer by 5 to 10 times) | 0.1mL
This virus titer boost is a novel recipe of small molecules designed for increasing / boosting lentivirus packaging efficiency. Lentivirus 10X Titer-Up stably regulates the viral RNA packaging on the transcriptional level, which can enhance production of lentiviral particles for up to 10 fold.
Supply Solutions Sigma Aldrich - Streptavidin Magnetic Particles, 2 ml * Third Party Item
Sigma Aldrich - Streptavidin Magnetic Particles, 2 ml * Third Party Item
Sigma Aldrich Fine Chemicals Biosciences Thrombin human BioUltra re
Thrombin is the final coagulation protease in regard to hemostasis promoting both procoagulant and anticoagulant effects.
Invivogen Ultrapure flagellin from B . Subtilis, 50 ug
Flagellin, the principal component of the flagella present on many Gram-negative and Gram-positive bacteria, is a proinflammatory molecule recognized by distinct types of pattern recognition receptors (PRRs); the surface localized Toll-like receptor (TLR5) and the cytosolic NOD-like receptors (NLRs), NLRC4 and NAIP5.
New England Biolabs, Inc. SNAP-Biotin – 50 nmol
SNAP-Biotin is suitable for applications such as biotinylation of SNAP-tag fusion proteins in living cells for detection with streptavidin fluorophore conjugates or labeling in solution for analysis by SDS-PAGE/Western blot or for capture with streptavidin for binding and interaction studies.
New England Biolabs, Inc. BG-PEG-NH2 – 2 mg
BG-PEG-NH2 is an amine-terminated building block for the one-step synthesis of SNAP-tag substrates from NHS esters or other activated carboxyl esters on labels or surfaces.
New England Biolabs, Inc. BG-GLA-NHS – 2 mg
BG-GLA-NHS is an amine-reactive building block for the one-step synthesis of SNAP-tag substrates from amine-containing precursors including proteins, peptides or oligonucleotides.
New England Biolabs, Inc. O-Glycosidase – 10000000 units
O-Glycosidase, also known as Endo--N-Acetylgalactosaminidase, catalyzes the removal of Core 1 and Core 3 O-linked disaccharides from glycoproteins.
New England Biolabs, Inc. SNAP-Surface 549 – 50 nmol
SNAP-Surface 549 is a photostable red fluorescent substrate that can be used to label SNAP-tag fusion proteins in solution or on the surface of living cells.
Rockland Immunochemicals ALP Conjugate Stabilizer (Azide and Mercury Free) - MB-061-0100
ALP Conjugate Stabilizer is to be used with calf intestinal Alkaline Phosphatase (ALP) conjugated primary or secondary antibodies or other proteins or compounds in solution. The components of the stabilizer (proprietary) are unique, and stabilize both the antibody and enzyme portions of the conjugate. ALP Conjugate Stabilizer prevents the loss of catalytic activity and maintains the active conformation of the antibody portion of the conjugate.
Enzo Life Sciences ATP (Energy) Regeneration Solution (100µl)
This solution contains MgCl2, ATP, and ATP regenerating enzymes to recycle ATP hydrolysis products, (AMP, ADP) to ATP. An ideal energy source for conjugation/degradation assays (ubiquitin, proteasome, SUMO, etc.) particularly those that are preformed with crude lysates/impure reagents that may contain ATP-depleting activities. Formulation: Liquid. In 50mM TRIS-Cl, pH 7.5. Long Term Storage: -80°C.
Genscript Corporation 2X Taq Master Mix
2X Taq Master Mix is a premixed 2X concentrated solution of Taq DNA Polymerase (GenScript, Cat. No. E00007), reaction buffer, MgCl 2 and dNTPs. 2X Taq Master Mix contains all components for PCR, except DNA template and primers. The mixture is optimized for consistent and efficient routine PCR amplifications. It can amplify up to 8 kb fragment from lambda DNA. For a 50 µl reaction, simply add 25 µl of 2X Taq Master Mix to primers, DNA template and PCR-Qualified H2O.
