Miscellaneous Molecular Biology Reagents
MP Biomedicals™ 3,3-Diaminobenzidine Tablets
3,3'-Diaminobenzidine is used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. Insoluble end product is resistant to alcohol and fading, making it ideal for immunohistochemistry. Each tablet contains 5 mg of DAB.
MilliporeSigma™ Calbiochem™ D(+)Cellobiose
Substrate for β-glucosidase
MilliporeSigma™ Calbiochem™ Chondroitin Sulfate, Bovine Trachea
Fundamental component of connective tissue
MilliporeSigma™ Calbiochem™ DNase I, Bovine Pancreas
Acts on single and double stranded DNA
MilliporeSigma™ Calbiochem™ Hypoxanthine
Used in conjunction with thymidine and aminopterin for cloning and selection of hybridoma cell lines
MilliporeSigma™ Calbiochem™ Ethidium Bromide Adsorber
Safe and simple removal of ethidium bromide (EtBr) from aqueous staining solutions
MilliporeSigma™ Calbiochem™ FURA 2/AM
Cell-permeable ester form of FURA 2
MilliporeSigma™ Calbiochem™ β-Glucan, Saccharomyces cerevisiae
Shown to induce oxidative burst response from NR8383 cells
MilliporeSigma™ Novabiochem™ Oxidizing Reagent, 0.05M, I2 in Pyridine
Used in biosynthesis.
Invitrogen™ dNTP Mix (2.5 mM)
The same high-quality deoxynucleotide triphosphate solutions provided in the Invitrogen™ RETROscript™ First Strand cDNA Synthesis Kit for RT-PCR.
Invitrogen™ UTP Soln. (10 mM)
This Invitrogen ribonucleotide triphosphate (rNTP) solution is a component of the MAXIscript™ Kits and is supplied in one tube containing 100 μL.
Invitrogen™ MagMAX™ Wash Solution 2 Concentrate
Invitrogen MagMAX Wash Solution 2 Concentrate is a component of all the MagMAX Isolation Kits that may be purchased separately.
BD Pharmingen™ Indo-1 AM Dye
Cell-permeant dye that can be used to measure intracellular ionized calcium concentration, [Ca2+]i. Indo-1 AM is a ratiometric dye: with UV excitation, the ratio of emission around 410nm to the emission around 485nm can be used to measure intracellular ionized calcium concentration.
Thermo Scientific™ X-Gluc
Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
MP Biomedicals™ IPTG Powder
Induces of α-galactosidase activity in bacteria; suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedure