Gene Reporter Systems
Promega NanoLuc™ pNL2 Vectors
Configured with NanoLuc Luciferase for use in reporter gene assays of transcriptional control—ideal for cloning of known or putative promoter region and establishment of stable cell line through Hygromycin selection
Promega ITK Kinase Enzyme System
Recombinant human ITK (amino acids 352-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ITK is a member of the TEC family of nonreceptor tyrosine kinases.
Promega Proteasome-Glo™ Bioluminescent Enzyme Assays
The Proteasome-Glo Assays are homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format (not suitable for cell-based applications).
Promega PKCγ Kinase Enzyme System
Recombinant full-length human PKC gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC gamma is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Promega Intracellular TE Nano-Glo Substrate/Inhibitor, 10,000 assays
The Intracellular TE Nano-Glo Substrate/Inhibitor is a supplement for NanoBRET™ Target Engagement Assays.
Promega cAMP-Glo™ Max Assay System
The cAMP-Glo™ Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate GPCRs coupled with adenylate cyclase typically alter intracellular cAMP levels.
Promega mFcgammaRIV ADCC Reporter Bioassay, Core Kit 5X, 1 each
The mFcgammaRIV ADCC Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the activity of mouse antibodies that specifically bind and activate FcgammaRIV.
Promega Calpain-Glo™ Assay System
The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (μ) and 2 (m) activities
Promega Beta-Glo™ Assay System
The Beta-Glo™ Assay System is a homogeneous, luminescence-based method for quantitating beta-galactosidase expression in mammalian cells.
Promega PKCα Kinase Enzyme System
Recombinant full-length human PKC alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKC alpha is a member of the protein kinase C (PKC) family of serine- and threonine-specific protein kinases.
Promega Ultra Pure UDP-GlcNAc, 100mM, 50microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega PKCδ Kinase Enzyme System
Recombinant full-length human PKC delta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C delta is a member of the protein kinase C (PKC) family of serine-threonine kinases.
Promega Calpain-Glo™ Assay System
The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (μ) and 2 (m) activities
Promega PDGFRβ Kinase Enzyme System
Recombinant human PDGFR beta (amino acids 557-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR beta is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.
Promega ADP-Glo™ Kinase Assay
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity.