Protein Resins
G-Biosciences Recombinant Protein A/G
Recombinant Protein A/G contains four Fc-binding domains from Protein A and two from Protein G but lacks the albumin binding sites.
Sigma Aldrich Fine Chemicals Biosciences Protein A-Sepharose(R) from Staphylococcus aureus lyophilized powder | MFCD00165563 | 1G
Protein A-Sepharose(R) from Staphylococcus aureus lyophilized powder | MFCD00165563 | 1G
Sigma Aldrich Fine Chemicals Biosciences MabSelectTM PrismA Cytiva
MabSelect PrismA affinity chromatography resin has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein A-derived ligand. This allows future demands in mAb purification to be met including processing of many bispecific antibodies..nbspEnhanced dynamic binding capacity allows high mass throughput of processed mAb per resin volume unit..nbspExcellent alkaline stability enables efficient cleaning and sanitization using 0.5-1.0 M NaOH for improved process economy bioburden control and robustness.The enhanced alkaline stability of MabSelect PrismA also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bioburden control for continuous processes which can be exposed to days or weeks of feed harvest.
Sigma Aldrich Fine Chemicals Biosciences Gammabind Plus SepharoseR
GammaBind Plus Sepharose(R) is used for lab-scale affinity purification of IgG from a large variety of species and has better binding to rat and mouse IgG subclasses compared to GammaBind Sepharose.GammaBind Plus Sepharose(R) is GammaBind G Type 3 covalently immobilized to Sepharose(R) CL-6B by malimide linkage. GammaBind G Type 3 a recombinant form of streptococcal protein G binds to the Fc region of IgG from a variety of mammalian species. GammaBind Plus Sepharose(R) may be used to analyze and purify classes subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium. Since only the Fc region is involved in binding the Fab region is still available for binding antigen.
Sigma Aldrich Fine Chemicals Biosciences HiTrapR MabSelectTM PrismA
These HiTrap columns are prepacked with MabSelect PrismA protein A chromatography resin. This affinity resin in the packed column has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein ligand allowing efficient cleaning between monoclonal antibody purification runs. This allows future demands in monoclonal antibody purification to be met including processing of many bispecific antibodies.Enhanced dynamic binding capacity compared with other protein A resins.Excellent alkaline stability enables efficient cleaning and sanitization using 0.5-1.0 M NaOH.Convenient HiTrap format for easy connection to a syringe peristaltic pump or chromatography systems such as an A„KTA system for convenient process optimization.The enhanced alkaline stability of MabSelect PrismA also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bi
Sigma Aldrich Fine Chemicals Biosciences HiScreenTM MabSelectTM SuR
HiScreen(TM) MabSelect(TM) SuRe(TM) is prepacked with MabSelect(TM) SuRe(TM) and is part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening for capture of monoclonal antibodies (MAbs).
Sigma Aldrich Fine Chemicals Biosciences HiTrapR MabSelectTM Cytiva
HiTrap(R) MabSelect(TM) columns are prepacked with MabSelect a Bioprocess medium for capuring of Mabs from large sample volumes.
Sigma Aldrich Fine Chemicals Biosciences Protein G SepharoseR fast
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex affinity chromatography on Sepharose-coupled human IgG and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10 with the strongest binding at pH 4 and 5 and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.P3296-5Ml updated product number is GE17-0618-01
Sigma Aldrich Fine Chemicals Biosciences EZviewTM Red Protein G Aff
EZ view Red Protein G Affinity Gel is Protein G covalently bonded to 4% Agarose beads. The affinity gel is used to bind the fC portion of the IgG of an antibody to samples such as cell lysates and tissue for purification of proteins in preparation of immunoprecipitation assays. Red dye enhances visability for more efficient results.
Sigma Aldrich Fine Chemicals Biosciences Itk Protein active 10 18
Product Source Human Itk expressed by baculovirus in Sf21 cells.
Sigma Aldrich Fine Chemicals Biosciences HiTrapR MabSelect SuReTM C
HiTrap(R) MabSelect(TM) SuRe(TM) columns are prepacked with MabSelect(TM) SuRe a BioProcess medium for purification of monoclonal antibodies from large sample volumes. The novel alkali-tolerant recombinant Protein A ligand allow cleaning-in-place (CIP).HiTrap(R) MabSelect(TM) columns are prepacked with MabSelect(TM) a bioprocess medium for capuring of Mabs from large sample volumes.
Sigma Aldrich Fine Chemicals Biosciences HiScreen MabSelectTM Prism
This protein A column is prepacked with MabSelect PrismA chromatography resin. This affinity resin has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein ligand allowing efficient cleaning between runs. This allows future demands in monoclonal antibody purification to be met including processing of many bispecific antibodies.Enhanced dynamic binding capacity compared with other protein A chromatography resins.Excellent alkaline stability enables efficient cleaning and sanitization using 0.5-1.0 M NaOH.Convenient method optimization and parameter screening enabled by the 10 cm bed height for efficient chromatography process development.The enhanced alkaline stability of MabSelect PrismA also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bioburden control for continuous processes which can be exposed to days or we
Sigma Aldrich Fine Chemicals Biosciences MabSelectTM SuReTM Cytiva
MabSelect(TM) SuRe(TM) is a protein A affinity medium for capturing MAbs at process scale. MabSelect(TM) SuRe(TM) has an alkali-tolerant rProtein A ligand that allows the use of rigorous and cost-effective CIP and sanitization protocols based on 0.1 to 0.5 M NaOH. Further the ligand is protease stable leading to lower ligand leakage.MabSelect(TM) SuRe(TM) is a member of the MabSelect(TM) family of affinity chromatography media for the capture of monoclonal antibodies (MAbs) at process scale. MabSelect(TM) SuRe(TM) shares features and benefits of MabSelect(TM) such as a rigid high-flow agarose matrix that ensures excellent pressure/flow properties low non-specific binding that leads to low impurity levels in the eluate pool and an oriented ligand coupling for optimal binding capacity. In addition MabSelect(TM) SuRe(TM) features an alkali-tolerant rProtein A ligand. This ligand provides greater stability than conventional protein A-based media in the alkaline conditions used in clea
Sigma Aldrich Fine Chemicals Biosciences MabSelectTM PrismA Cytiva
MabSelect PrismA affinity chromatography resin has been improved with an optimized high-flow agarose base matrix and a genetically engineered protein A-derived ligand. This allows future demands in mAb purification to be met including processing of many bispecific antibodies.Enhanced dynamic binding capacity allows high mass throughput of processed mAb per resin volume unit.Excellent alkaline stability enables efficient cleaning and sanitization using 0.5-1.0 M NaOH for improved process economy bioburden control and robustness.The enhanced alkaline stability of MabSelect PrismA also provides opportunities for more robust mAb purification using periodic counter-current chromatography. The possibility for cleaning using 1 M NaOH increases bioburden control for continuous processes which can be exposed to days or weeks of feed harvest.