General Cell Based Assays
Cayman Chemical Glycerol CellBased Assay Kit
Cayman’s Glycerol Cell-Based Assay provides a convenient tool for studying triglycerides/fatty acid cycling and its regulation in adipocytes or hepatocytes. This kit will allow investigators to screen compounds involved in lipid storage and metabolism. Chloroquine is included in the kit as a positive control for screening pharmaceuticals that induce lipid droplet accumulation and free glycerol release from hepatocytes.
Eurofins Discoverx PathHunter Human UTR2 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human UTR2 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure UTR2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress CXCR6 HEK 293 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0205E1CP0M)
The PathHunter CXCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL16) based activation of CXCR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR6 and EA-tagged β-Arrestin-2. Activation of CXCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM1 Activated GPCR Internalization Assay, 2pl Kit. (93-0655E3CP5M)
The PathHunter CHRM1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CHRM1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CHRM1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Enzo Life Sciences ROS-ID Hypoxia/Oxidative stress detection kit (1x500tests)
Specific detection system for simultaneous analysis of hypoxia and oxidative stress using microscopy and flow cytometry. Enzo Life Sciences’ ROS-ID.The Hypoxia (Red) dye takes advantage of the nitroreductase activity present in hypoxic cells by converting the Nitro group to hydroxylamine (NHOH) and amino (NH.The Oxidative Stress Detection Reagent is a non-fluorescent, cell-permeable total ROS detection dye which reacts directly with a wide range of reactive species. The generated fluorescent products can be visualized using a wide-field fluorescence microscope equipped with standard fluorescein (490/525 nm) and Texas Red (596/670 nm) filters, confocal microscopy, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser. Applications: Flow Cytometry. Fluorescence microscopy. Fluorescent detection, HTS. Contents: Hypoxia Red Detection Reagent. Oxidative Stress Detection Reagent (Green). ROS Inducer (Pyocyanin). Hypoxia Inducer (DFO)
Eurofins Discoverx PathHunter eXpress c-Ret-GFRα1 Functional Assay, 10pl Kit. (93-1054E3CP16L)
The PathHunter eXpress c-Ret-GFRα1 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated c-Ret and GFRα1 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged c-Ret and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, GFRα1 Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the cynomolgus version of the protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems CellQuanti-MTT™ Cell Viability Assay Kits. Homogeneous assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents.
Homogeneous assay for cell viability, proliferation, cytotoxcity and evaluation of anticancer agents. Key Features: Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 950 cells can be accurately quantified. Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS. Z factors of 0.5 and above are observed. Can be readily automated with HTS liquid handling systems. Method: OD570nm. Samples: Cell culture. Species: All. Procedure: Assay takes 5 hrs, hands-on time 30 min. Kit size: 500 tests. Detection limit: 950 cells.
Bioassay Systems EnzyChrom™ Histamine Assay Kit. For quantitative determination of histamine in food, beverage and agricultural products.
For quantitative determination of histamine in food, beverage and agricultural products. Key Features: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.0025 - 1 mM (0.3 - 111 ppm) histamine in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: OD565nm. Samples: Food, beverage and agricultural products. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 2.5 µM.
Hach Company SC4500 Ctrl Prof DP 2 Analog C
SC4500 Controller-Profibus DP-2 Analog UPW Conductivity Sensors-100-240 VAC-without power cordIncludes Controller-Non-Claros Embedded Prognosys-without power cord includes mounting hardware
Eurofins Discoverx AssayComplete Cell Plating 38 Reagent, 500. (93-0563R38B)
AssayComplete Cell Plating 38 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Cayman Chemical LIpoxygenase InhIbItr Screen
Cayman’s Lipoxygenase Inhibitor Screening Assay Kit detects and measures the hydroperoxides produced in the lipoxygenation reaction using a purified LO. The detection reaction is equally sensitive to hydroperoxides at various positions within the fatty acid, and will work with fatty acids of any carbon length. It is thus a general detection method for LO, and can be used to screen libraries of compounds for those which inhibit LO enzymes.Provided in this kit is arachidonate 15-LO purified from soybean, which can be used to screen test compounds. Alternatively, if other purified LOs (provided by the user) are to be used for screening, the included enzyme can be used as a control. Both arachidonic and linoleic acids are included as substrates. The preferred substrate for screening will depend on the LO being tested. It is recommended to use the fatty acid substrate best suited for the LO being tested.
Indigo Biosciences Human Granulocyte-Macrophage Colony-Stimulating Factor Receptor (GM-CSFR, CSF2R) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Granulocyte-Macrophage Colony-Stimulating Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against GM-CSFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results