General Cell Based Assays
Eurofins Discoverx PathHunter Epoetin Alfa Bioassay Kit, 2-Plate. (93-0965Y3-00017)
The PathHunter Epoetin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses erythropoetin or epoetin alfa-induced dimerization of EPOR receptors. This product uses EFC technology. The cell line included overexpresses EPOR target protein fused to PK alongside EPOR target protein fused to EA. Upon ligand-induced dimerization of EPOR, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Cayman Chemical ε-NADP+ (sodium salt), Purity: ≥95%, 3.8 mg
A fluorescent derivative of NADP+; ex/em = 310/400 nm, respectively; has been used to determine the binding stoichiometry of NADP+ to FASN
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3906641 TRIS USP GMP HIGH ASSAY
American Research Products Inc Human Actin related protein 2
Human Actin-related protein 2/3 compleX subunit 2 (ARPC2) ELISA kit from CUSABIO Detection Range 23 5 pg/mL-1500 pg/mL Sensitivity 5 8 pg/mL Sample serum plasma tissue homogenates cell lysates Method Sandwich
Eurofins Discoverx AssayComplete Cell Plating 32 Reagent, 100. (93-0563R32A)
AssayComplete Cell Plating 32 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Meso Scale Discovery RPLEX HUM Lactotransferrin ASY
The Meso Scale Discovery R-PLEX Human Lactotransferrin Assay QuickPlex 5-PL Kit is a multiplex immunoassay that quantitatively measures lactotransferrin in human serum plasma saliva milk and cell culture supernatant samples The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and rapid results This 5-plex kit includes a plate coated with capture antibodies detection antibodies calibrators controls assay and wash buffers substrate and plastic consumables needed to run the assay The kit can test up to 80 samples in duplicate and has a testing time of approximately 3 5 hours
Apexbio Technology LLC Red Fluorometric Lipid Droplet Assay Kit 500T
Red Fluorometric Lipid Droplet Assay Kit is a fluorescent detection kit designed for the visualization and quantification of intracellular lipid droplets It is intended to probe and monitor lipid metabolism by specifically labeling intracellular neutral lipid accumulations This kit exerts its function primarily through the use of Nile Red a cell-permeable lipophilic dye that selectively accumulates within neutral lipid environments resulting in red fluorescence upon excitation (552 nm emission 636 nm) No key metric (IC50/EC50) or specific cell line information is available Based on these properties Red Fluorometric Lipid Droplet Assay Kit enables research applications in cellular lipid metabolism autophagy cell differentiation and senescence
Eurofins Discoverx PathHunter Human P2RY2 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human P2RY2 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure P2RY2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human SSTR4 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human SSTR4 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure SSTR4 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GHR-JAK1 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human GHR-JAK1 Stable Cell Line Functional Kinase Assay (U2OS) measures GHR cytokine receptor activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human OPRM1 (Opioid mu) β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human OPRM1 (Opioid mu) β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure OPRM1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Mouse IL-6 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-6 Assay Kit is a sensitive immunoassay for detecting mouse interleukin 6 (IL-6) in various samples It uses MSD s electrochemiluminescent technology offering high sensitivity a wide dynamic range and quick results The kit includes a 96-well plate with capture antibodies for mouse IL-6 detection antibodies with an electrochemiluminescent label and calibrators for a standard curve Sample incubation is 2 hours with no wash steps quantifying IL-6 from 0 2-10 000 pg/mL
Meso Scale Discovery R-PLEX Human cIAP1 ASY
The R-Plex Human CIAP1 Assay from Meso Scale Diagnostics is a multiplex immunoassay that measures the levels of Cellular Inhibitor of Apoptosis Protein 1 (CIAP1) in human serum plasma and cell culture supernatants This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The assay can monitor CIAP1 levels across four orders of magnitude from sub-picogram to nanogram levels per mL This quantitative immunoassay allows the study of CIAP1 levels in biological samples for life science research
Eurofins Discoverx PathHunter eXpress HTR1F U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0699E3CP0M)
The PathHunter HTR1F eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1F GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1F and EA-tagged β-Arrestin-2. Activation of HTR1F stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress CXCR3 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0054E2CP2M)
The cAMP Hunter CXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR3, a naturally Gi/Go coupled GPCR. Upon activation of CXCR3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.