General Cell Based Assays
Molecular Devices Assay Kit, Calcium 6, Evaluation, Fluorescence, 96/384/1536-well
The FLIPR Calcium 6 Evaluation Kit provides a homogeneous, fluorescence-based method to detect intracellular calcium changes in cell-based assays. It is formulated for use with compatible fluorescence plate readers and supports multiple microplate formats.
- Homogeneous fluorescence assay for intracellular calcium detection.
- Evaluation kit sized for three assay plates and three QF assay plates.
- Includes 3 vials of component A, 3 vials of component C for QF, and 1 bottle of assay buffer.
- Compatible with 96-well, 384-well, and 1536-well microplates.
- Designed for screening GPCRs, ion channels, and calcium-mediated signaling.
- Each reagent vial is sufficient for one plate, depending on dispenser dead volume.
Eurofins Discoverx PathHunter eXpress GLP1R Total GPCR Internalization Assay, 2-Plate Kit. (93-1075E3CP5M)
The PathHunter GLP1R Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of GLP1R via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged GLP1R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GLP1R receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human CXCL11 (CXCR3) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human CXCL11 (CXCR3) Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure CXCR3 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical Cell-Based Assay Buffer Tablet, 1 ea
Cell-Based Assay Buffer Tablet has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Medchemexpress LLC GNE-987 GSH linker-1 | 2417370-39-7 | C62H77F2N9O12S4 | 10MG
GNE-987 GSH linker-1 is a PROTAC-linker conjugate that pairs a BET-degrading warhead with a disulfide-containing glutathione-cleavable linker for use in PROTAC-antibody conjugate (PAC) research. It is intended as a research reagent for chemical biology studies involving targeted protein degradation and intracellular payload release.
- Enables targeted protein degradation when conjugated to antibodies.
- Contains a disulfide-based glutathione-cleavable linker for intracellular release.
- High chemical purity suitable for analytical and biological testing.
- Compatible with common solvents and recommended storage protocols.
- Applicable to studies of protein degradation pathways and PAC development.
Eurofins Discoverx PathHunter eXpress EDNRA CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0257E2CP0M)
The PathHunter EDNRA eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-1) based activation of EDNRA GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDNRA and EA-tagged β-Arrestin-2. Activation of EDNRA stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems EnzyFluo™ NADP/NADPH Assay Kit. For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism (This product is a replacement for the discontinued product EFNP-100).
For quantitative determination of NADP and NADPH and evaluation of drug effects on their metabolism (This product is a replacement for the discontinued product EFNP-100). Key Features: Sensitive and accurate. Detection limit of 0.01 µM and linearity up to 1 µM NADP+/NADPH in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at time zero and 30 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Method: FL530/585nm. Samples: Cell, tissue extracts etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 0.01 µM.
Eurofins Discoverx PathHunter eXpress AVPR1B Activated GPCR Internalization Assay, 10pl Kit. (93-0722E3CP5L)
The PathHunter AVPR1B Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of AVPR1B via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged AVPR1B. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting AVPR1B-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human Tenascin C ASY
The R-PLEX Human Tenascin-C Assay from Meso Scale Discovery is an electrochemiluminescent immunoassay designed for the quantitative measurement of Tenascin-C in human serum plasma and cell culture supernatants This assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and rapid detection of Tenascin-C an extracellular matrix glycoprotein implicated in tissue injury and cancer progression With a detection range of 1 56-400 ng/mL and minimal required sample volume this assay allows high-throughput and accurate quantification of Tenascin-C levels from small sample sizes
Sigma Aldrich Fine Chemicals Biosciences L Lactate Assay Kit suffic
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is therefore a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple direct and automation-ready procedures for measuring lactate concentration are very desirable.
Cayman Chemical Cell-Based Assay Buffer Tablet, 5 ea
Cell-Based Assay Buffer Tablet has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Eurofins Discoverx PathHunter GLP2R Bioassay Kit, 10-Plate. (93-0572Y2-00134)
The PathHunter GLP2R Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. GLP2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress OPRM1 U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0213E3CP0L)
The PathHunter OPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. met 5-enkephalin) based activation of OPRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRM1 and EA-tagged β-Arrestin-2. Activation of OPRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR2C (VGV) U2OS β-Arrestin GPCR Assay, 10pl Kit. (93-0932E3CP19L)
The PathHunter HTR2C(VGV) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C(VGV) GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C(VGV) and EA-tagged β-Arrestin-2. Activation of HTR2C(VGV) stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
