General Cell Based Assays
Cell Biolabs Inc OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence), 5x96 assays
The OxiSelect™ In Vitro ROS/RNS Assay Kit is an assay for measuring the total free radical presence of a sample. The assay employs a proprietary quenched fluorogenic probe, DCFH-DiOxyQ), which is based on similar chemistry to the popular DCFH-DA. The DCFH-DiOxyQ probe is first primed with a quench removal reagent, and subsequently stabilized in the highly reactive DCFH form. In this reactive state, ROS and RNS species can react with DCFH, which is rapidly oxidized to the highly fluorescent 2’, 7’-dichlorodihydrofluorescein (DCF) . The DCFH-DiOxyQ probe can react with hydrogen peroxide, peroxyl radical, nitric oxide, and peroxynitrite anion. These free radical molecules are representative of both ROS and RNS, thus allowing for measurement of the total free radical population within a sample. The kit has a detection sensitivity limit of 40 nM for H2O2. Each kit provides sufficient reagents to perform up to 5x96 assays, including standard curve and unknown samples.
Eurofins Discoverx PathHunter Human PTHR1 Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human PTHR1 Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure PTHR1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human SSTR4 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human SSTR4 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure SSTR4 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Waters Corp Waters Tubing Assembly, MP35N, 0.062 in. x 0.004 in. x 25 in., Low Pressure
This replacement tubing assembly is designed for use in Bio Systems with a 30 cm column heater. It is constructed from MP35N material and features low pressure fittings.
- Constructed from MP35N material.
- Provided with low pressure fittings.
- Suitable for Bio Systems.
- Compatible with a 30 cm column heater.
Apexbio Technology LLC NAD/NADH-Assay-Kit-Colori
NAD /NADH Assay Kit (Colorimetric) is widely used in biomedical research to measure the balance of NAD and NADH essential cofactors in cellular metabolic pathways including glycolysis oxidative phosphorylation and the TCA cycle This kit s origin lies in the need to evaluate cellular redox states enabling researchers to study energy metabolism mitochondrial function and oxidative stress It operates by quantifying changes in absorbance correlating with NAD/NADH levels typically in the nanomolar to low micromolar range Common applications include in vitro studies using cultured cells animal models and drug screening to assess metabolic alterations Experimental concentrations typically depend on the specific research design allowing for flexibility in various scientific investigations
NEW EAST BIOSCIENCES LLC RhoA Pull-Down Activation Assay Kit, 30 Assays
The RhoA Activation Assay Kit uses configuration-specific anti-RhoA-GTP Mouse monoclonal antibody to measure RhoA-GTP levels in cell extracts or in vitro GTPγS loading RhoA activation assays. Anti-RhoA-GTP mouse monoclonal antibody is first incubated with cell lysates containing RhoA-GTP. Next, the GTP-bound RhoA is pulled down by protein A/G agarose. Finally, the precipitated RhoA-GTP is detected through immunoblot analysis using Anti-RhoA Rabbit Polyclonal Antibody.
Cell Biolabs Inc 24W WOUND HEALING TRIAL SIZE
The CytoSelect 24-Well Wound Healing Assay (Trial Size) provides more consistent results well-to-well compared to homemade scratch assays. Useful for measuring cell migration, cell proliferation and wound closure.
Cell Biolabs Inc Lipid Extraction Kit (Chloroform-Free), 50 preps
Traditional lipid extraction by the Folch method is tedious and uses toxic chloroform as the key solvent. In addition, the extracted lipids end up in the bottom layer which is inconvenient. Cell Biolabs' Lipid Extraction Kit (Chloroform-Free) uses an organic solvent system that results in the organic phase being on the top, making it easier to use while eliminating the need for chloroform. Each kit provides sufficient reagents for 50 preps based on a 100µL sample size.
Cell Biolabs Inc Free Fatty Acid Assay Kit (Colorimetric), 100 assays
Free fatty acids (FFA) are formed from the hydrolyisis of triglyceride ester bonds and serve as a ready energy source by binding to albumin in the bloodstream. Measurement of FFA has become an important part of monitoring various disease states including obesity, diabetes, and cancer. Cell Biolabs' Free Fatty Acid Assay Kit quantifies the FFA concentration (non-esterified) in various samples using a 96-well colorimetric plate format. It can detect FFA levels as low as 15 µM in plasma or serum samples. Each kit provides sufficient reagents for 100 assays including standards and unknown samples.
Abcam Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric)
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Abcam Chitosan Assay Kit (Colorimetric)
Chitosan Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Bioassay Systems QuantiChrom™ Bilirubin Assay Kit. For quantitative determination of bilirubin and evaluation of drug effects on bilirubin metabolism.
For quantitative determination of bilirubin and evaluation of drug effects on bilirubin metabolism. Key Features: Sensitive and accurate. Detection limit 0.16 mg/dL bilirubin in 96-well plate assay. Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 10 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Method: OD530nm. Samples: Serum. Species: All. Procedure: Assay takes 10 min. Kit size: 180 tests. Detection limit: 0.16 mg/dL.