General Cell Based Assays
TRIBIOSCIENCE INC Urea Colorimetric Assay 200 Tests
The Tribioscience Urea Colorimetric Assay provides a rapid, sensitive method to measure urea concentration in biological samples. The intensity of the color is directly proportional to the urea concentration in the sample.
Cayman Chemical UrIc AcId asy KIt 96Wel
Cayman’s Uric Acid Assay Kit provides fluorescence- or colorimetric-based methods for detecting uric acid in plasma, serum, and urine. In the assay, uricase catalyzes the conversion of uric acid to allantoin, hydrogen peroxide (H2O2), and carbon dioxide. H2O2, in the presence of horseradish peroxidase, reacts stoichiometrically with ADHP (10-acetyl-3,7-dihydroxyphenoxazine) to produce the highly fluorescent compound resorufin. Resorufin fluorescence can be analyzed with an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. Alternatively, the absorbance of resorufin can be measured at 570 nm.
Cayman Chemical nTosIs asy KIt 1ea
Cayman’s NETosis Assay Kit provides a simple and fast method for studying the process of NETosis ex vivo. Notably, Cayman’s NETosis Assay Kit does not depend upon the DNA component of NETs, as DNA release can occur independently of NETosis. In this kit, primary neutrophils are stimulated to release NETs with either PMA or a calcium ionophore (both included). As shown in Figure 1, unbound neutrophil elastase is washed away following NET generation. Following digestion of NET DNA by S7 nuclease, the supernatant containing neutrophil elastase is added to a substrate, which is selectively cleaved by neutrophil elastase to yield a 4-nitroaniline product that absorbs light at 405 nm. A sufficient amount of EAch reagent is provided to test and analyze up to 80 individual samples or 24 samples in duplicate.
Cayman Chemical nTosIs Imagng asy KIt 96Wel
Cayman’s NETosis Imaging Kit provides a simple staining protocol for visualizing the process of NETosis kinetically ex vivo and in vitro. A cell-permeable DNA dye allows visualization of the dynamic changes of the nucleus during the process of NETosis, while a cell-impermeable dye detects the extruded DNA. Combining the fluorescence visualization of these dyes with brightfield imaging in a high-content platform allows for exquisite differentiation between different modes of cell death. The amount of EAch reagent provided is sufficient for two 96-well plates.
Biochain Institute Inc NAD/NADH Assay Kit, 100 Assays/PK
BioChain's NAD+/NADH assay kit provides simple, direct and automation-ready procedure to measure NAD+/NADH concentration. It based on an alcohol dehydrogenase cycling reaction, in which a tetrazolium dye (MTT) is reduced by NADH in the presence of phenazine methosulfate (PMS). The intensity of the product color, measured at 565 nm, is proportionate to the NAD+/NADH concentration in the sample.
Bulldog Bio Inc ACCU CHIP KIT W/STAIN SOLUTION
ADAM MC AccuChip 4x Kit w/AccuStain T/N Solution (400 tests - cell viability, 800 tests - total cell count) MFR# AD4K-200
Hycult Biotech Inc LAL CHROMOGENIC ENDPOINT ASSAY
The LAL assays is to be used for the in vitro quantitative determination of bacterial endotoxin in various biological fluids (including sera), devices, (air)filters and tissue culture medium.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-Rb (Ser807/811) Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Phospho-Rb (Ser807/811) Detection Kit, 10,000 Assay Points
Eurofins Discoverx PathHunter eXpress PRLHR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0302E2CP2M)
The PathHunter PRLHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. PrRP) based activation of PRLHR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PRLHR and EA-tagged β-Arrestin-2. Activation of PRLHR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress SSTR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0181E2CP0L)
The PathHunter SSTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin) based activation of SSTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR2 and EA-tagged β-Arrestin-2. Activation of SSTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human & Mouse Total c-Src Detection Kit, 500 Assay Points
HTRF Human & Mouse Total c-Src Detection Kit, 500 Assay Points
Cayman Chemical TGR5GP-BAR1 Reporter asy 1ea
An IRE1a inhibitor (IC50s 0.2 and 0.02 UM for RNase and kinase activity respectively) selective for IRE1a over Ron and FGFR1 (IC50s 4.4 and 17 UM) as well as a panel of 282 kinases at 10 UM inhibits ER stress-induced increases in the splicing of mRNA encoding XBP1 in eight multiple myeloma cell lines at 8 UM reduces viral replication in tick-borne encephalitis virus-infected SH-SY5Y and SNB-19 cells at 0.5 UM and tick-borne encephalitis virus-infected Caco-2 cells at 1 UM
Bioassay Systems EnzyChrom™ Uric Acid Assay Kit II, Uric acid determination in biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts.
URIC ACID is the breakdown product of purines such as adenosine and inosine. Humans and other primates are incapable of further metabolizing uric acid. In humans, uric acid is excreted via the kidneys, but a failure to remove excess uric acid can lead to conditions such as gout or uric acid kidney stones. BioAssay Systems’ method provides a simple and high-throughput assay for measuring uric acid levels. In this assay, uric acid is enzymatically converted to allantoin, releasing H2O2. The resulting H2O2 reacts with a specific dye to form a pink-colored product. The change in OD570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to the uric acid present in the sample.
Revvity Health Sciences Inc DELFIA EuTDA Cytotoxicity Detection kit, 1 item
DELFIA EuTDA Cytotoxicity Detection kit, 1 item
TARGETMOL CHEMICALS INC P38 MAPK INHIBITOR 5MG
Also available in 1 mg 10 mg 25 mg 50 mg 100 mg 500 mg and bulk. Please contact Fisher for quotes. p38 MAPK inhibitor is a potent inhibitor of p38 MAP kinase (IC50 = 35 nM). It inhibits senescence induced by the oncogene RAS. purity: 99%