General Cell Based Assays
Eurofins Discoverx PathHunter eXpress XBP1 Nuclear Translocation Assay, 10pl Kit. (93-0857E3CP5L)
The PathHunter eXpress XBP1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the IRE1/XBP1 pathway. This kit assesses ligand (e.g. thapsigargin) based pathway activation via detection of resulting XBP1 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged XBP1 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of XBP1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress VIPR2 Total Internalization Assay, 10pl Kit. (93-0824E3CP5L)
The PathHunter VIPR2 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of VIPR2 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged VIPR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the VIPR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Dojindo Molecular Technologies Inc NAD/NADH Assay Kit-WST (100TESTS), enables quantification of the amount of total NAD+/NADH, NAD+ and NADH in cells and measurement of their ratio.
Recently, it has become clear that Sirtuin is linked to longevity and plays a role in NAD+ level regulation. Also Sirtuin has been recognized as a marker necessary to understanding biological states, such as obesity & diabetes, as well as cellular differentiation. Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD+ levels can be determined by subtracting NADH levels from independently measured total NAD+/NADH levels. NAD/NADH Assay Kit-WST enables quantification of the amount of total NAD+/NADH, NADH and NAD+ in cells and measurement of their ratio. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
Meso Scale Discovery R-PLEX Human NCAM-1 ASY
The R-PLEX Human NCAM-1 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures NCAM-1 (Neural Cell Adhesion Molecule 1) in human serum plasma and cell culture supernatants This assay kit utilizes MSD s electrochemiluminescent detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The kit includes a 96-well plate pre-coated with capture antibody detection antibody Read Buffer Calibrator Blend and Sample Diluent The assay has a typical lower limit of detection of 0 05 ng/mL and an upper limit of 500 ng/mL for NCAM-1
Cell Biolabs Inc Urea Assay Kit, 200 assays
Cell Biolabs' Urea Assay Kit is a simple method for quantifying urea in a convenient 96-well microplate format. The kit detects urea levels as low as 0.78 mg/dL in plasma, serum, urine, cell lysates, or tissue homogenates. Each kit provides sufficient reagents for 200 assays including standards and unknown samples.
iQ Biosciences Human Treg Suppression Assay Kit w/ CFSE-Labeled CD8 T Cells - 5 pk
Human Treg functional kits with Regulatory T cells and CD8 T cell responders. CD8 T cells are CFSE-labeled for downstream functional readout. Treg cells are validated for regulatory function. Used to test molecules for modulation of Treg function. Carefully cryopreserved to ensure high viability (> 90%) upon thawing. All orders come with an iQ Certificate of Analysis
Cayman Chemical AconItase IsocItrIc Dehydroge
A Bax activator (Ki 43.3 nM in a fluorescence polarization assay) selectively reduces viability in wild-type but not Bax-/- MEFs at 25 UM inhibits nicotine-induced increases in phosphorylated Bax levels in A549 cells at 5 UM reduces viability in A549 cells at 5 UM induces cell cycle arrest at the G2/M phase in U87MG cells at 5 10 or 15 UM disrupts the mitochondrial membrane potential in U87MG cells at 15 UM induces cytochrome c release from mitochondria and Bax insertion into mitochondrial membranes in A549 cells at 5 or 10 UM decreases tumor volume in an A549 mouse xenograft model at 40 or 60 mg/kg per day
Eurofins Discoverx InCELL Hunter eXpress CLK1 Kinase Binding Assay, 10pl Kit. (96-0059E1CP0L)
The InCELL Hunter eXpress CLK1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of CLK1 via detection of CLK1 levels. This product uses EFC technology. The cell line included overexpresses CLK1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Montana Molecular Green Cilia-targeted cADDis cAMP Assay - 10mL
Measure real-time cAMP signaling kinetics in the primary cilia with this live cell fluorescent assay. Add 5HT6-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates ciliary cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cilia-targeted cAMP sensor BacMam and 500mM sodium butyrate in H2O.
Meso Scale Discovery RPLEX HUM PDGFRB total ASY
The R-PLEX Human PDGFR- Total Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures the total levels of human platelet-derived growth factor receptor beta (PDGFR- ) in cell and tissue lysates using electrochemiluminescence detection technology This assay kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker solutions calibrator and positive control lysate The assay has a detection range of 50-50 000 pg/mL and exhibits minimal cross-reactivity with other growth factors This product enables high-throughput quantification of total PDGFR- levels for research applications
Eurofins Discoverx InCELL Hunter eXpress HEK 293 SMARCA4 Bromodomain Assay, 10-plate Kit. (96-0060E1CP7L)
The InCELL Hunter eXpress HEK 293 SMARCA4 Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of SMARCA4 via detection of SMARCA4 levels. This product uses EFC technology. The cell line included overexpresses SMARCA4 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
TRIBIOSCIENCE INC NADH/NAD Quantification Colorimetric Assay Kit, 100 Tests
NADH/NAD Quantification Colorimetric Assay provides the easiest and most accurate approach to measuring NADH from a variety of samples, which can be measured at OD 460 nm.
Revvity Health Sciences Inc HTRF Human & Mouse Total eIF2α Detection Kit, 10,000 Assay Points
HTRF Human & Mouse Total eIF2α Detection Kit, 10,000 Assay Points
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-NF-κB (Ser536) Detection Kit, Control Lysate
HTRF Human & Mouse Phospho-NF-κB (Ser536) Detection Kit, Control Lysate
Eurofins Discoverx InCELL Hunter eXpress PIM1 Kinase Binding Assay, 2pl Kit. (96-0052E15CP0M)
The InCELL Hunter eXpress PIM1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of PIM1 via detection of PIM1 levels. This product uses EFC technology. The cell line included overexpresses PIM1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.