Protein Purification Reagents and Kits
Genscript Corporation Antibody Resin Protein Purification Protein Purification 5 ML 1/EA
GenScripts MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is designed for optimal purification of DYKDDDDK-tagged proteins.
Cayman Chemical Monoacylglycerol Lipase Inhib
Selectively and reversibly blocks MAGL activity (IC50s = 0.18 and 59 µM for MAGL and FAAH in mouse brain, respectively); does not inhibit ABHD6, ABHD12, CB1, or CB2 receptors (Kis > 10 µM); used to ameliorate disease progression in a mouse model of multiple sclerosis
AdipoGen ANGPTL8 msFc hrec
Protein. Mouse ANGPTL8 Betatrophin 22-198 is fused at the C-terminus to the Fc portion of human IgG1. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Lyophilized. Contains PBS 0.02% CHAPS. Purity >90% SDS-PAGE. ANGPTL8 Angiopoietin-like protein 8 RIFL Lipasin Betatrophin is a newly discovered secreted protein of 198 aa that was proposed to promote beta cell proliferation and improve glucose tolerance in mice. ANGPTL8 may also function in inhibition of lipase activity and on serum triglyceride regulation. ANGPTL8 is expressed in the liver and in white and brown adipose tissue of mice. In humans, ANGPTL8 is found to be predominantly expressed in the liver. ANGPTL8 levels are reduced by fasting and are elevated upon insulin resistance and during pregnancy. ANGPTL8, according to preliminary data could bind to the macrophage receptor Marco and also to RTN4R, a neuronal receptor.
Cayman Chemical 1012 ZConjUGated LInole 25mg
An isomer of linoleic acid; has been found in bovine milk products; binds to PPARα (IC50 = 200 nM) and activates PPARα in a reporter assay using COS-1 cells expressing the mouse receptor; inhibits stearoyl-CoA desaturase activity in mouse liver microsomes and TRAP activity in media from primary human CD14+ monocytes; induces apoptosis in LoVo and HT-29 colon cancer cells at 100 µM; reduces epididymal, perirenal, and subcutaneous white adipose tissue weights in hamsters fed an atherogenic diet
Genscript Corporation Antibody Resin Protein Purification Protein Purification 1 ML 1/EA
GenScript Anti-His Affinity Resin is designed for purification of His-tagged protein. It is composed of agarose and conjugated THETM anti-His monoclonal antibody. His-tagged protein in the crude samples can bind to the anti-His monoclonal antibodies coupled with the agarose and the nonspecific proteins can be eliminated by washing. As a result the His-tagged protein will finally be eluted with high recovery. Moreover this product can be used for immunoprecipitation of His-tagged protein.
Cayman Chemical Soluble EpoxID Hydrolase Cel
Mammalian sEH is a member of the α/β-hydrolase fold family of enzymes that catalyze the hydrolysis of exogenous and endogenous epoxides to vicinal diols. Endogenous substrates for sEH include EETs which exhibit vasodilatory and anti-inflammatory activity. Cayman’s sEH Cell-Based Assay provides a convenient 96-well plate, fluorescence-based method for detecting epoxide hydrolase activity in whole cells. The assay utilizes Epoxy Fluor 7, a sensitive fluorescent substrate for sEH that can be used to monitor the activity of both human and murine enzymes.
Abcam x AV939, Tankyrase inhibitor, 25MG
MW 312.31 g/mol, Purity >98%. Potent tankyrase (TNKS) inhibitor. Inhibitor of Wnt/β-catenin signaling. (IC₅₀ values are 11 and 4 nM for TNKS1 and 2, respectively). Inhibits TNKS PARP activity. Selectivity over other PARP enzymes and CRE, NK-κB and TGF-β.
The product is subject to the following: Abcam Restricted Use Statement
Cell Biolabs Inc Urinary Creatinine Assay Kit, 192 assays
Creatine and p-creatine are converted non-enzymatically to creatinine, which enters the blood and is excreted by the kidneys via glomerular filtration. The analysis of creatinine in serum and urine is an important clinical test for renal disease and dysfunction. Creatinine is removed from plasma by the glomerulus and then excreted in the urine without any appreciable resorption by the tubules. This “creatinine clearance” from the body is used to measure glomerular filtration rates. Cell Biolabs’ Urinary Creatinine Assay Kit is based on the Jaffe reaction, produces an orange-red color complex that can be measured with a standard spectrophotometric plate reader. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, creatinine standards and unknown urine samples. High concentrations of ascorbic acid, uric acid, glucose, ketones and cephalosporin antibiotics may interfere with the assay causing falsely high values.
STEMCELL Technologies Mitochondrial Isolation Kit, Size: 50 Tests
Conveniently enrich the mitochondrial or cytosolic subcellular fractions from mammalian cells or tissues using the Mitochondrial Isolation Kit. This kit offers two quick options to isolate the mitochondrial subcellular fraction and has been verified for use with western blot analysis. The first option is a reagent-based method for processing up to six samples concurrently. The second option utilizes Dounce homogenization, providing a two-fold increase in isolated mitochondria from a single sample. Both options use a microcentrifuge-based method to separate the mitochondrial fraction from the cytosolic fraction while preserving their biological activity. This separation is a valuable tool for studying apoptosis and signaling events between the two fractions. For more information on the Mitochondrial Isolation Kit, visit www.stemcell.com.
Abcam ChIP Kit - One Step, 96 Tests
Abcam's ChIP Kit - One Step (ab117138) enables the user to enrich a chromatin fraction containing specific DNA sequences from isolated chromatin to be able to investigate the interaction between proteins and DNA.
The product is subject to the following: Abcam Restricted Use Statement
Cayman Chemical 4hydroxy Nonal GlutathIon 50UG
An internal standard for the quantification of 4-HNE-GSH by GC- or LC-MS
Cayman Chemical 4-hydroxy Nonenal Glutathione
An internal standard for the quantification of 4-HNE-GSH by GC- or LC-MS
Cayman Chemical S-NItroso-L-glutathIon 100mg
S-Nitroso-L-glutathione is an S-nitrosothiol NO donor based on naturally-occurring glutathione. It is a potent smooth muscle relaxant and inhibitor of platelet aggregation. Its stability in solution varies from seconds to hours depending on temperature, buffer composition, and metal content.{1277,5064,4274,3632}
MilliporeSigma Total Protein Extraction Kit, 1 kit
Total protein extraction kit is an excellent tool for the initial purification and preparation of proteins from any tissues and cells. Total protein isolated by this kit is native and can be used for downstream applications.
Genscript Corporation Enterokinase, His, Bovine
Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.