Protein Purification Reagents and Kits
System Biosciences LLC PEG-it Virus Precipitation Solution, 100ml
PEG-it Virus Precipitation Solution (100 mL aliquot)
Takara Bio PrimeSTAR® Max DNA Polymerase, 100 Rxns
A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio's high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition. 100 Rxns
IBA LifeSciences Twin-Strep-tag Capture Kit for SPR analysis; Classic size
Twin-Strep-tag Capture Kit is intended for site-directed, reversible capture of Twin-Strep-tag fusion proteins for biomolecular interaction analysis using surface plasmon resonance (SPR) devices. The kit provides Strep-TactinXT, SPR Immobilization Buffer, Regeneration Buffer, and GFP-Twin-Strep-tag as control protein. It enables the highly stable immobilization of the ligand based on the unique Strep-TactinXT:Twin-Strep-tag interaction, which operates with pM affinity. The key benefits are:• Indirect immobilization protecting the ligand from chemical modification as well as inactivation or manipulation by the immobilization procedure• High affinity enabling measurements over a long period• Chip can be regenerated easily by an established procedure• Ligands can be captured directly from complex samples due to the high affinity and specificityThe use of unconjugated Strep-TactinXT requires a license that is provided with the purchase of the kit as limited use license.
Pfanstiehl Inc Maltose Monohydrate. High Purity, Low Endotoxin, Low Metals, USP-NF, JP, ChP (M-167) -100G GMP, Multi-compendial CAS: 6363-53-7
Pfanstiehl Maltose Monohydrate High Purity, Low Endotoxin, Low Metals is a multi-compendial carbohydrate utilized in bioprocessing applications and as an infusion stabilizer. Pfanstiehl Maltose has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Maltose meets parenteral (injectable) excipient grade standards, making it ideal as a bioprocessing feed, or as a stabilizing agent for pharmaceutical infusions. Pfanstiehl Maltose is available as USP-NF (National Formulary) JP (Japanese Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade .
IBA LifeSciences Strep-TactinXT 4Flow resin as 50% suspension; 20 ml
Strep-TactinXT 4Flow is a 4% agarose coupled with the streptavidin variant Strep-TactinXT and applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose allows for the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow enables the application of denaturing agents such as urea. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
Wl Gore And Associates 1.0 mL GORE Protein Capture Device with Protein A for Rapid mAb Purification
GORE Protein Capture Devices with immobilized Protein A are intended for small-scale affinity purification of monoclonal antibodies and other proteins demonstrating an Fc region. GORE Protein Capture Devices use a unique expanded polytetrafluoroethylene (ePTFE) membrane composite that provides a binding capacity advantage at high flow rates and improved the speed of purification. In addition the GORE Protein Capture Deivces produce highly concentrated elution pools, which may eliminate a downstream concentration step during the antibody purification process. These unique membrane devices are offered in 1 mL (≥ 30 mg capacity), 3.5 mL (≥ 105 mg capacity) and 9.0 mL (≥27 mg capacity) at 20 seconds residence time.
Protifi LLC S-Trap mini kit (10x 100 – 300 ug)
S-Trap sample processing is now available in a full kit format. The kit includes lysis, wash, and digestion buffers. The user must supply HPLC grade MeOH.
KAYAKU ADVANCED MATERIALS INC MICROPOSIT 1165 Remover, Clear colorless liquid, 4 x 4 L/CS, Solubility in water: Complete, Hazard classification: Combustible liquid, Flammable liquid, NMP concentration: 94-95%
MICROPOSIT 1165 Remover, Clear colorless liquid, 4 x 4 L/CS, Solubility in water: Complete, Hazard classification: Combustible liquid, Flammable liquid, NMP concentration: 94-95%
STEMCELL Technologies EasySep™ Mouse B Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-B cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using and EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For isolation of B cells expressing CD11b or CD43, we recommend using the EasySep™ Mouse Pan-B Cell Isolation kit (Catalog #19844 (https://www.stemcell.com/products/easysep-mouse-pan-b-cell-isolation-kit.html)).This product replaces the EasySep™ Mouse B Cell Enrichment Kit (Catalog #19754) for even faster cell isolations.
CUBE BIOTECH purecube 100 compact cartridge ni-indigo 1x1 ml
purecube 100 compact cartridge ni-indigo 1x1 ml
STEMCELL Technologies EasySep™ Human T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human T Cell Isolation Kit is designed to isolate T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This product can be used in place of the EasySep™ Human T Cell Enrichment Kit (Catalog #19051) for even faster cell isolations.
Epigentek Inc EpiMag HT (96-Well) Magnetic Separator (1/Pack)
The EpiMag HT (96-Well) Magnetic Separator is a magnetic stand (magnetic rack) that allows paramagnetic bead precipitation of liquid samples from various flat-bottom or U-bottom 96-well microplates (we recommend our EpiMag 96-Well Microplates for ideal compatibility). It can be used for isolation and purification of nucleic acids and proteins, immunoprecipitation, immunoassays (ELISA), cell sorting, and purification of biomolecules. A PCR adapter is also included for the option of using V-bottom semi-skirted/unskirted PCR microplates.
Hampton Research SOLUBILITY & STABILITY SCREEN
HR2-072/Solubility & Stability Screen - 0.5 ml, Deep Well block format. Please note items from this supplier are non-returnable.