Tissue Lysates
Sino Biological Recombinant Human Claudin-4/CLDN4 Protein (Nanodisc, Full-length, Avi & His Tag), Avi-tag Biotinylated
A DNA sequence encoding the Human CLDN4 (O14493) (Ala2-Val209) was expressed with an AVI tag at the N-terminus and a polyhistidine tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.
