Restriction Enzymes
New England Biolabs, Inc. PvuI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence CG_AT^CG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. ApaI – 5000 units
A restriction endonuclease that recognizes the sequence G_GGCC^C.
New England Biolabs, Inc. BsaXI – 100 units
A restriction endonuclease that recognizes the sequence _NNN^NNNNNNNNNACNNNNNCTCCNNNNNNN_NNN^.
New England Biolabs, Inc. ApeKI – 250 units
A restriction endonuclease that recognizes the sequence G^CWG_C.
New England Biolabs, Inc. BstBI – 12500 units
A restriction endonuclease that recognizes the sequence TT^CG_AA.
New England Biolabs, Inc. HindIII – 10000 units
A restriction endonuclease that recognizes the sequence A^AGCT_T.
New England Biolabs, Inc. PvuII-HF – 25000 units
A high fidelity restriction endonuclease that recognizes the sequence CAG^CTG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. MboI – 2500 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. HinP1I – 2000 units
A restriction endonuclease that recognizes the sequence G^CG_C.
New England Biolabs, Inc. BglII – 10000 units
A restriction endonuclease that recognizes the sequence A^GATC_T.
New England Biolabs, Inc. Sau3AI – 200 units
A restriction endonuclease that recognizes the sequence ^GATC_.
New England Biolabs, Inc. XmaI – 2500 units
A restriction endonuclease that recognizes the sequence C^CCGG_G.
New England Biolabs, Inc. StuI – 1000 units
A restriction endonuclease that recognizes the sequence AGG_^CCT.
New England Biolabs, Inc. XcmI – 5000 units
A restriction endonuclease that recognizes the sequence CCANNNN_N^NNNNTGG.
New England Biolabs, Inc. TspRI – 1000 units
A restriction endonuclease that recognizes the sequence _NNCASTGNN^.