Restriction Enzymes
New England Biolabs, Inc. Endo Hf – 500000 units
Endo Hf is a recombinant protein fusion of Endoglycosidase H and maltose binding protein. Endo Hf cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins equally as well as Endo H.
New England Biolabs, Inc. BsgI – 50 units
A restriction endonuclease that recognizes the sequence GTGCAGNNNNNNNNNNNNNN_NN^.
New England Biolabs, Inc. PspGI – 1000 units
A restriction endonuclease that recognizes the sequence ^CCWGG_.
New England Biolabs, Inc. HpyCH4V – 100 units
A restriction endonuclease that recognizes the sequence TG_^CA.
New England Biolabs, Inc. I-CeuI – 500 units
A restriction endonuclease that recognizes the sequence CGTAACTATAACGGTC_CTAA^GGTAGCGAA.The intron encoding I-CeuI is present in the chloroplast large rRNA gene of Chlamydomonas eugametos (1). This gene has been cloned and overexpressed in E. coli (2).
New England Biolabs, Inc. PciI – 200 units
A restriction endonuclease that recognizes the sequence A^CATG_T.
New England Biolabs, Inc. EnGen Spy dCas9 (SNAP-tag) – 400 pmol
EnGen Spy dCas9 is an inactive mutant of Cas9 nuclease that retains programmable DNA binding activity. EnGen Spy dCas9 contains two point mutations, D10A and H840A, which inactivate its RuvC and HNH nuclease domains, respectively. The N-terminal SNAP-tag allows for covalent attachment of fluorophores, biotin, and a number of other conjugates useful for visualization and target enrichment. Like with Cas9 nuclease, DNA binding by EnGen Spy dCas9 requires a guide RNA complementary to a target sequence with a protospacer adjacent motif (PAM), NGG, downstream of the target sequence. EnGen Spy dCas9 contains a SV40 T antigen nuclear localization sequence (NLS) at the C- terminus of the protein.
New England Biolabs, Inc. EnGen Spy Cas9 Nickase – 70 pmol
EnGen Spy Cas9 Nickase is a variant of Cas9 nuclease differing by a point mutation (D10A) in the RuvC nuclease domain, which enables it to nick, but not cleave, DNA). EnGen Spy Cas9 Nickase, like EnGen Cas9 NLS , targets DNA using a guide RNA complementary to a site with a 3' NGG protospacer adjacent motif (PAM). Nicking occurs on the DNA strand opposite the PAM. Double-stranded DNA breaks can be generated with reduced off-target cleavage by targeting two sites in close proximity (generally 0-20 bp apart) and with PAMs facing outward to leave 5' overhangs. Nicking by EnGen Spy Cas9 Nickase occurs on the DNA strand opposite the target sequence. EnGen Spy Cas9 Nickase contains SV40 T antigen nuclear localization (NLS) sequences on the N and C- termini.
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I FLTRD-25MG
Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.
New England Biolabs, Inc. HinfI – 25000 units
A restriction endonuclease that recognizes the sequence G^ANT_C.
New England Biolabs, Inc. MspA1I – 500 units
A restriction endonuclease that recognizes the sequence CMG_^CKG.
New England Biolabs, Inc. Hpy99I – 100 units
A restriction endonuclease that recognizes the sequence _CGWCG^.
New England Biolabs, Inc. PstI – 10000 units
A restriction endonuclease that recognizes the sequence C_TGCA^G.