Restriction Enzymes
New England Biolabs, Inc. RNase Inhibitor, Human Placenta – 2000 units
RNase Inhibitor, Human Placenta is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor, Human Placenta is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014.
New England Biolabs, Inc. Endonuclease V – 250 units
Endonuclease V is a repair enzyme found in E. coli that recognizes deoxyinosine, a deamination product of deoxyadenosine in DNA. Endonuclease V, often called deoxyinosine 3 endonuclease, recognizes DNA containing deoxyinosines (paired or not) on double-stranded DNA, single-stranded DNA with deoxyinosines and to a lesser degree, DNA containing abasic sites (ap) or urea, base mismatches, insertion/deletion mismatches, hairpin or unpaired loops, flaps and pseudo-Y structures. It is believed that Endonuclease V needs another protein to repair the DNA, as it does not remove the deoxyinosine or the damaged bases. Endonuclease V cleaves the second phosphodiester bonds 3' to the mismatch of deoxyinosine, leaving a nick with 3'-hydroxyl and 5'-phosphate.
New England Biolabs, Inc. CviQI – 10000 units
A restriction endonuclease that recognizes the sequence G^TA_C.
New England Biolabs, Inc. Terminal Transferase – 500 units
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.
New England Biolabs, Inc. Nt.BspQI – 1000 units
A restriction endonuclease that recognizes the sequence GCTCTTCN^.
New England Biolabs, Inc. EcoP15I – 500 units
A restriction endonuclease that recognizes the sequence CAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN^NN_.
New England Biolabs, Inc. PspXI – 1000 units
A restriction endonuclease that recognizes the sequence VC^TCGA_GB.
New England Biolabs, Inc. NmeAIII – 250 units
A restriction endonuclease that recognizes the sequence GCCGAGNNNNNNNNNNNNNNNNNNN_NN^.
New England Biolabs, Inc. FatI – 250 units
A restriction endonuclease that recognizes the sequence ^CATG_.
New England Biolabs, Inc. SalI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^TCGAC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. HindIII-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence A^AGCT_T. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BamHI-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence G^GATC_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. MluI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence A^CGCGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BtsI-v2 – 500 units
BtsI-v2 is an engineered and improved version of BtsI that recognizes the sequence GCAGTGNN^.
New England Biolabs, Inc. BssSI-v2 – 200 units
BssSI-v2 is an engineered and improved version of BssSI that recognizes the sequence VC^TCGAGB. BssSI-v2 is an engineered and improved version of BssSI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)