Restriction Enzymes
New England Biolabs, Inc. EagI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence C^GGCC_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. SwaI – 2000 units
A restriction endonuclease that recognizes the sequence ATTT_^AAAT.
New England Biolabs, Inc. BspQI – 500 units
A restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_.
New England Biolabs, Inc. SmaI – 10000 units
A restriction endonuclease that recognizes the sequence CCC_^GGG.
New England Biolabs, Inc. NdeI – 20000 units
A restriction endonuclease that recognizes the sequence CA^TA_TG.
New England Biolabs, Inc. HindIII-HF – 50000 units
A high fidelity restriction endonuclease that recognizes the sequence A^AGCT_T. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
INTACT GENOMICS INC PEmax Enzyme 400 µg
Intact Genomics (IG) PEmax enzyme is the purified recombinant Streptococcus pyogenes Cas9 nickase mutant (H840A)-Murine Moloney Leukemia Virus-Reverse Transcriptase (MMLV-RT) fusion protein3-4 containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR prime-editing3-4. The physical purity of this enzyme is ≥85% as assessed by SDS-PAGE with Coomassie blue staining.Product Includes: PEmax enzyme 5x PEmax Nicking Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM MgCl2, 0.1 mM EDTA, pH 6.5 @ 25 °CStore all components at -20°C.
New England Biolabs, Inc. HpaII – 2000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. PciI – 1000 units
A restriction endonuclease that recognizes the sequence A^CATG_T.
New England Biolabs, Inc. EcoRV-HF – 4000 units
A high fidelity restriction endonuclease that recognizes the sequence GAT_^ATC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Terminal Transferase – 500 units
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. Protruding, recessed or blunt-ended double or single-stranded DNA molecules serve as a substrate for TdT. The 58.3 kDa enzyme does not have 5' or 3' exonuclease activity. The addition of Co2+ in the reacton makes tailing more efficient.
New England Biolabs, Inc. SphI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. BbsI – 300 units
A restriction endonuclease that recognizes the sequence GAAGACNN^NNNN_.
ABclonal Technology ClaI
5...A T/ C G A T...3 3...T A G C/ T A...5 Isoschizomers: Bsu15I Bsa29I BseCI BshVI BspDI BsuTUI
New England Biolabs, Inc. Exonuclease V (RecBCD) – 5000 units
Exonuclease V, a RecBCD complex from E. coli has several different enzyme activities, including an ATP-dependent single-stranded DNA endonuclease activity, ss- and ds- DNA exonuclease activity. The hydrolysis in each case is bi-directional (from both the 3' and 5' ends) and processive, producing oligonucleotides. All Exonuclease V activities have divalent cation requirements. Mg2+ is required for the exonuclease activity, while Ca2+ inhibits the exonuclease activity and allows double-stranded DNA unwinding (helicase activity) without hydrolysis.