Substrates
Vector Laboratories DAB Peroxidase (HRP) Substrate Kit (With Nickel), 3,3'-Diaminobenzidine 300 ml
The Vector Laboratories DAB Peroxidase Substrate Kit with Nickel includes a nickel solution that can change the reaction product color from brown to gray/black. This kit provides all of the necessary reagents in convenient dropper bottles and works well for both IHC and blot-based protein detection. Slides developed with DAB/ Ni should be dehydrated, cleared, and permanently mounted. This kit can be used to prepare 300 ml of working solution. Vector Laboratories horseradish peroxidase (HRP) substrate kits include options that are cost-effective (e.g. standard DAB), sensitive (e.g ImmPACT DAB) and convenient (ImmPACT DAB EqV). The range of vibrant colors available facilitates multiplex staining and optimum contrast with pigmented tissues and counterstains.
Surmodics Inc TMB One Component HRP Microwell Substrate, 1L
The BioFX™ one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution. Peroxidase reacts with the TMB substrate solution to produce a soluble blue reaction product that can be read at 370 nm or 620-650 nm for ELISA applications. Stop solutions can be added to prevent further color development and reading the absorbance values at the desired wavelengths.
Medchemexpress LLC Solutol HS-15, 61909-81-7, 50 g
Formula: (C₂H₄O)nC₁₈H₃₆O3. Solutol HS-15 is a permeability enhancer. Store at 4°C, sealed storage, away from moisture.
Vector Laboratories ImmPACT™ VIP Peroxidase (HRP) Substrate Kit 120 ml
Vector Laboratories ImmPACT VIP Peroxidase (HRP) Substrate Kit produces an intense violet (purple) reaction that is 2-4 times more sensitive than that of Vector VIP Substrate. The Vector ImmPACT VIP chromogen provides an excellent color contrast in pigmented tissues such as melanoma or retina and can be used for both manual and automated staining methods. This kit consists of 120 ml of diluent and concentrated stock solutions in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4 degrees C. Stained sections should be dehydrated, cleared, and permanently mounted.
Surmodics Inc TMB One Component HRP Membrane Substrate, 1L
The BioFX™ one component 3,3’,5,5’-tetramethylbenzidine (TMB) substrate is supplied in a ready-to-use solution for membrane applications. The staining pattern provides a dark blue precipitate with low background to increase the signal-to-noise ratios in western blot, dot blots and line assays.
Gold Biotechnology Inc D-Luciferin, Potassium Salt (Proven and Published™) 100 mg
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Surmodics Inc BCIP/NBT Purple One Component AP Membrane Substrate, 1L
The substrate is a ready to use, one-component formulation of 5-bromo-4-chloro-3-indolylphosphate and nitroblue tetrazolium for use in immunoblotting or other membrane applications.
Gold Biotechnology Inc D-Luciferin, Potassium Salt (Endotoxin Free) 1g
Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
Vector Laboratories ImmPACT DAB EqV Peroxidase (HRP) Substrate | 100mL
ImmPACT DAB EqV consists of equal volumes of Reagent 1 and Reagent 2, and when mixed together form a working solution. ImmPACT DAB EqV produces a dark brown reaction product and is 3 to 5 times more sensitive than the original DAB substrate.
Sigma Aldrich Fine Chemicals Biosciences Locust bean gum from Ceratonia siliqua seeds, 9000-40-2, MFCD00131257, 500 g
Locust bean gum (LBG, galactomannan polysaccharide) from Ceratonia siliqua seeds may be used as a substrate to help identify, differentiate and characterize mannanase(s), such as the β-Mannanases (EC 3.2.1.78). LBG may be used in the development of drug delivery devices.
Advansta Inc WesternBright Sirius Chemiluminescent Detection Kit 200ml
WesternBright Sirius is the most sensitive HRP substrate available from Advansta for chemiluminescent Western blotting. With attomole sensitivity and a long-lasting signal, WesternBright Sirius allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.
Seracare Life Sciences Inc TMB SUBSTRATE SOL B 1X100ML
Most sensitive chromogenic substrate for peroxidase detection, more sensitive than ABTS and OPD. It develops a deep blue color in the presence of peroxidase-labeled conjugates measurable at 650 nm. Turns yellow when stopped by acidification and read at 450 nm. Recommended for ELISA and blotting applications. 2-component format of 3, 3', 5, 5' - Tetramethylbenzidine, simply mix and use. Has longer shelf life than 1-component TMB substrates.
Vector Laboratories AEC Peroxidase (HRP) Substrate Kit 300 ml
AEC Peroxidase (HRP) Substrate Kit (3-amino-9-ethylcarbazole) produces a red reaction product that can be used as a single label or as a second color for multiple antigen labeling using either manual or automated IHC staining methods. Stained slides must be aqueously mounted (e.g. in VectaMount AQ). This kit contains stock solutions in convenient dropper bottles, including all of the reagents necessary to prepare about 300 ml of working solution (2-3000 sections). It can be also used for staining on blots. Vector Laboratories horseradish peroxidase (HRP) substrate kits include options that are cost-effective (e.g. standard DAB), sensitive (e.g. ImmPACT™ DAB) and convenient (ImmPACT DAB EqV). The range of vibrant colors available facilitates multiplex staining.
Vector Laboratories ImmPACT NovaRED™ Peroxidase (HRP) Substrate Kit 120 ml
ImmPACT NovaRED™ Peroxidase (HRP) Substrate produces an intense red reaction product, with 2-4X the sensitivity of standard VECTOR NovaRED substrate. Sections stained with NovaRED can be dehydrated, cleared, and permanently mounted. This chromogen is useful as an alternative to DAB or as a second color for multiple antigen labeling. ImmPACT NovaRED Substrate also provides excellent color contrast in pigmented tissue such as melanoma and can be used for both manual and automated staining methods. ImmPACT NovaRED™ substrate produces a red reaction product for use in immunohistochemical applications. This product generates 120 ml of working solution that is stable for up to 14 days when stored at 4 degrees C.