Substrates
Medchemexpress LLC H-Glu(AMC)-OH | 98516-76-8 | MFCD00038085 | 95.0% | 304.30 g/mol | C15H16N2O5 | 25 MG
H-Glu(AMC)-OH is an amino acid derivative used as a fluorogenic peptide substrate to measure aminopeptidase A activity. It releases the fluorescent leaving group 7-amino-4-methylcoumarin (AMC) upon enzymatic cleavage, enabling sensitive fluorometric assays for biochemical research. The material is supplied as a powder for laboratory use.
- Fluorogenic substrate for aminopeptidase A activity assays.
- Releases 7-amino-4-methylcoumarin (AMC) upon enzymatic cleavage for sensitive fluorescence detection.
- Powder form suitable for preparation of assay solutions.
- Stable when stored as a powder at -20°C (up to 3 years) or 4°C (up to 2 years).
- In-solution stability: short-term storage at -80°C (months) or -20°C (weeks) depending on solvent.
- Molecular formula C15H16N2O5; molecular weight 304.30 g/mol.
Medchemexpress LLC H-Asp(AMC)-OH | 133628-73-6 | MFCD00236804 | 99.8% | 290.27 g/mol | C14H14N2O5 | 5 MG
H-Asp(AMC)-OH is an amino acid derivative bearing a 7-amino-4-methylcoumarin (AMC) fluorophore, provided as a solid for use as a fluorescent substrate or dye in biochemical assays. It has defined molecular properties and is supplied with high reported purity suitable for research applications.
- Fluorescent AMC-labeled aspartic acid derivative.
- High purity (≈99.8%).
- Molecular weight 290.27 g/mol.
- Molecular formula C14H14N2O5.
- Solid, white to off-white powder.
- Available in milligram packaging for assay use.
- Reported not to inhibit glycine transport at 0.25 mM.
VAZYME INTERNATIONAL LLC Add&Read Phospho-ERK1/2 (Thr202/Tyr204) Kit, 500 tests
This kit is designed for the detection of phosphorylated ERK protein content in cells. It utilizes the Add&Read TR-FRET no-wash ELISA methodology, offering a streamlined two-step workflow that significantly saves time and labor compared to traditional ELISA methods.
- Based on TR-FRET methodology (Time-Resolved Fluorescence Resonance Energy Transfer).
- Employs a two-step workflow.
- Saves over 80 percent of time and labor compared to traditional ELISA methods.
- Utilizes a no-wash ELISA assay type.
- Designed for the detection of phosphorylated ERK protein content in cells.
- Provided in a 500-test size.
Medchemexpress LLC Z-Nle-Lys-Arg-AMC acetate | 1135364-14-5 | 99.6% | 706.83 | C36H50N8O7.xC2H4O2 | 10 MG
Z-Nle-Lys-Arg-AMC acetate is a fluorogenic peptide substrate that specifically monitors cathepsin B activity across a broad pH range. Supplied as a solid powder for research use, it produces a fluorescent signal upon enzymatic cleavage, enabling sensitive activity assays and inhibitor screening.
- Monitors cathepsin B activity across a broad pH range.
- Generates fluorescent signal upon enzymatic cleavage for sensitive assays.
- High purity (99.58%) with molecular weight 706.83 for consistent performance.
- White to off-white solid suitable for sealed storage.
- Stable under recommended storage: powder at -80°C (2 years) or -20°C (1 year); in solvent at -80°C (6 months) or -20°C (1 month).
- Supplied in 10 mg quantity for laboratory assays.
MEDILUMINE INC MediLumine Inc. D-Luciferin Potassium Salt, 1 g
Highly pure D-Luciferin potassium salt. This potassium salt is a water soluble form of D-Luciferin. D-Luciferin is a common bioluminescent substrate used for imaging of firefly luciferase expression with peak chemiluminescent emission at 560 nm. The ATP dependant luciferin oxidation is catalyzed by the Luciferase enzyme encoded by luc gene.
Medchemexpress LLC Boc-Gln-Ala-Arg-AMC (acetate) | 00-00-0 | 99.7% | 690.74 g/mol | C31H46N8O10 | 5 MG
Boc-Gln-Ala-Arg-AMC acetate is an AMC-labeled peptide substrate for fluorescence-based protease assays. Enzymatic cleavage liberates the AMC fluorophore, producing a measurable fluorescent signal used in activity assays and screening applications.
- Fluorogenic peptide substrate for protease activity assays.
- Releases fluorescent AMC upon enzymatic hydrolysis.
- Validated for trypsin and TMPRSS2 activity measurement.
- High purity (99.7%) by HPLC.
- Available in small pack sizes suitable for assay development.
Medchemexpress LLC D-luciferin sodium | 103404-75-7 | MFCD00044938 | 99.9% | 302.30 | C11H7N2NaO3S2 | 5MG
D-Luciferin sodium is the sodium salt of D-luciferin, a bioluminescent substrate for firefly luciferase. Upon oxidation in the presence of luciferase, ATP, and oxygen it produces a yellow-green light (peak ~560 nm), making it suitable for sensitive reporter assays and bioluminescence imaging. Supplied as a light-yellow solid with high purity and a defined molecular weight for consistent assay performance.
- High purity for reliable assay results.
- Generates a strong yellow-green bioluminescent signal (peak ~560 nm).
- Suitable for in vitro and in vivo luciferase reporter assays.
- Sodium salt form for good aqueous solubility and easy preparation.
- Supplied in small, accurately weighed quantities for precise dosing.
Medchemexpress LLC D-luciferin sodium salt | 103404-75-7 | MFCD00044938 | 99.9% | 302.3 g/mol | C11H7N2NaO3S2 | 5G
D-luciferin sodium is the water-soluble sodium salt of firefly luciferin used as a chemiluminescent substrate for luciferase-based assays and imaging. It supplies a reliable light-emitting substrate for quantitative enzymatic assays and bioluminescent imaging applications.
- Water-soluble sodium salt for easy assay preparation.
- Suitable as a chemiluminescent substrate for luciferase-based assays and imaging.
- High purity (99.9%) for reproducible results.
- Soluble in water for preparation of assay solutions.
- Stable as a solid when stored sealed, away from moisture and light.
- Solution storage: -80°C up to 6 months or -20°C up to 1 month.
- Molecular weight 302.3 g/mol; formula C11H7N2NaO3S2.
- Available in lab-scale pack sizes including 5 g.
Medchemexpress LLC D-luciferin (sodium) | 103404-75-7 | 99.9% | 302.30 | C11H7N2NaO3S2 | 500MG
D-Luciferin sodium is the sodium salt of D-luciferin, the natural substrate for firefly luciferase used to generate bioluminescent signals in reporter assays and imaging. Supplied as a light yellow solid with high purity, it is suitable for in vitro and in vivo bioluminescence applications.
- Natural substrate for firefly luciferase.
- Produces bioluminescent signal for reporter assays and imaging.
- High purity (99.9%).
- Molecular weight 302.30.
- Formula C11H7N2NaO3S2.
- Available in multiple pack sizes, including 500 MG.
- Solid, light yellow to yellow appearance.
- Store sealed, away from moisture and light; in solvent store at -80°C (6 months) or -20°C (1 month).