Signal Transduction Reagents and Kits

BD Phosflow™ Violet Fluorescent Cell Barcoding Kit

Provides reagents for successfully barcoding T cell subsets from human whole blood samples for studying phosphorylated protein induction in MAP Kinase and JAK/STAT signaling pathways

Promega™ ADP-Glo™ Kinase Assay

One assay for all your kinase screening needs. The assay is well suited for measuring the effects of chemical compounds on the activity of many purified kinases, making it ideal for primary screening as well as kinase selectivity profiling.

Alfa Aesar™ Ionomycin Calcium Salt, 99%

Highly selective and potent calcium ionophore

Promega HDAC-Glo Class IIa Assay, 10ml

The HDAC-Glo Class IIa and HDAC-Glo 2 Assays are single-reagent-addition homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) Class IIa and Class I enzyme 2 from cells, extracts or purified enzyme sources.

Promega™ ONE-Glo™ Assay

A reagent that is more robust and stable when reconstituted, and less aromatic than
standard reagents created with luciferin

Promega Kinase Selectivity Profiling System: CMGC-2, 8 x 50 reactions

Easy-to-use kits for performing kinase selectivity profiling that rely on the ADP-Glo Kinase Assay technology. Each system includes kinase and substrate pairs in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.

Promega™ ADP-Glo™ Kinase Assay + AKT3 Kinase Enzyme System

For measuring effects chemical compounds have on activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling

Promega™ ADP-Glo™ Kinase Assay + CDK6/CyclinD3 Kinase Enzyme System

For measuring effects chemical compounds have on activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling

Alfa Aesar™ Sulindac Sulfide

Active metabolite of sulindac; inhibits cyclooxygenase-1

Alfa Aesar™ NF-kB Activation Inhibitor VI, BOT-64

A cell-permeable benzoxathiole compound that acts as an IKK-2 inhibitor

Promega HDAC-Glo™ I/II Assay and Screening Systems

A single-reagent-addition,homogeneous, luminescent assay that measures the relative activity of histone
deacetylase (HDAC) class I and II enzymes from cells, extracts or purified enzyme
sources.

Promega MST1 Kinase Enzyme System, 10microg

Recombinant full-length human MST1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MST1 belongs to a family of proteins that share similarity with a budding yeast serine/threonine kinase, sterile-20 (Ste20).

Promega CDK6/CyclinD3 Kinase Enzyme System, 10microg

Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.

Trypsin Inhibitor, chicken egg whites

Inhibits the pancreatic enzyme trypsin

Promega PAK4 Kinase Enzyme System, 10microg

Recombinant full-length human PAK4 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. PAK4 is a member of the p21-activated kinases (PAKs), which have been implicated in the regulation of cell morphology, motility and transformation.

Tacrolimus, ≥99%

Interacts with the immunophilins cyclophilin and FKBP-12

Promega NIK Kinase Enzyme System, 10microg

Recombinant human NIK (325-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NIK is a mitogen-activated protein kinase kinase kinase 14 (MAP3K14), which binds to TRAF2 and stimulates NF-kappaB activity.

Promega DYRK2 Kinase Enzyme System, 10microg

Recombinant full-length human DYRK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Promega TBK1 Kinase Enzyme System, 10microg

Recombinant full-length human TBK1 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases.

Promega IKK alpha Kinase Enzyme System, 10microg

Full-length recombinant human IKK alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKK alpha is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein.

Alfa Aesar™ N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide

A colorimetric substrate for caspase-3

Alfa Aesar™ Tropicamide, ≥99%

A muscarinic antagonist

RITA, p53 activator III

A cell-permeable, p53-targeting, tricyclic thiophene derivative

Promega PI:3PS Lipid Kinase Substrate, 0.5mg, 0.5ml

The lipid kinase substrates PIP2:3PS and PI:3PS are standalone substrates that are also part of the PI3K-Glo kinase profiling system.

Elastase Inhibitor II

A potent irreversible inhibitor of human neutrophil elastase

U0126, ≥99%

Selective inhibitor of mitogen-activated protein kinases MEK-1 and MEK-2

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