Crosslinking Reagents

Thermo Scientific™ Pierce™ EMCH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this mid-length (11.8A) crosslinker containing maleimide and hydrazide groups.

Thermo Scientific™ Pierce™ NHS-Azide Staudinger Ligation Reagents Available on GSA/VA Contract for Federal Government customers only.

Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.

Thermo Scientific™ Pierce™ Sulfo-NHS (N-hydroxysulfosuccinimide) No-Weigh™ Format

Thermo Scientific™ Pierce™ Sulfo-NHS (N-hydroxysulfosuccinimide) No-Weigh™ Format is a chemical modification reagent for converting carboxyl groups to amine-reactive NHS esters for bioconjugation, crosslinking, labeling and immobilization methods.

Thermo Scientific™ Pierce™ Sulfo-SANPAH Crosslinker

Crosslink amines to anything with this long crosslinker composed of Sulfo-NHS-ester and nitrophenyl azide reactive groups; photoactivates with UV light (Amax 320 to 350nm).

Thermo Scientific™ Pierce™ Sulfo-SIAB Crosslinker

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom spacer arm.

Thermo Scientific™ Pierce™ DSG Crosslinker

Crosslink amines to amines with disuccinimidyl glutarate with NHS-ester groups at the ends of a 7.7A spacer arm; membrane-permeable for intracellular protein crosslinking.

Thermo Scientific™ Pierce™ BMH Crosslinker

Crosslink sulfhydryls to sulfhydryls (cysteine residues in proteins or peptides) with this long crosslinker composed of maleimide groups and 11-atom (13.0A) spacer arm.

Thermo Scientific™ Pierce™ SMCC Crosslinker

Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 8-atom spacer arm.

Thermo Scientific™ Pierce™ Sulfo-NHS Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.

Thermo Scientific™ Pierce™ DMP Crosslinker

Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.

Thermo Scientific™ Pierce™ SPDP Crosslinker

Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups on 3-atom spacer arm; forms cleavable disulfide bonds with cysteines.

Thermo Scientific™ Pierce™ NHS-Diazirine Crosslinkers Available on GSA/VA Contract for Federal Government customers only.

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Thermo Scientific™ Pierce™ Photoreactive Amino Acids Available on GSA/VA Contract for Federal Government customers only.

Diazirine analogs of leucine and methionine to express proteins in cell culture that will crosslink their protein interactors upon UV-light activation in vivo.

Thermo Scientific™ Pierce™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) No-Weigh™ Format

Thermo Scientific™ Pierce™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) No-Weigh™ Format is a water-soluble, amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium cyclohexane spacer arm.

Thermo Scientific™ Pierce™ EDC Crosslinker

Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.

Thermo Scientific™ Pierce™ LC-SPDP Crosslinker

Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups at ends of 10-atom (15.7A) spacer arm.

Thermo Scientific™ Pierce™ SIA Crosslinker

Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 3-atom (1.5A) spacer arm.

Thermo Scientific™ Pierce™ BM(PEG)3 Crosslinker

Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 3-unit (17.8A) PEG spacer arm.

Thermo Scientific™ Pierce™ Sulfo-SMCC Crosslinker

Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.

Thermo Scientific™ Pierce™ PDPH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars via cleavable disulfide bonds using this short crosslinker containing pyridyldithiol and hydrazide groups.

Thermo Scientific™ Pierce™ PEG12-SPDP Crosslinker

Very long (54.1A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 12-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.

Thermo Scientific™ Pierce™ DSS (disuccinimidyl suberate) No-Weigh™ Format

Thermo Scientific™ Pierce™ DSS (disuccinimidyl suberate) No-Weigh™ Format is noncleavable and membrane permeable crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm.

Thermo Scientific™ Pierce™ DSP Crosslinker Available on GSA/VA Contract for Federal Government customers only.

Crosslink amines to amines with dithiobis succinimidyl propionate, composed of NHS-ester groups and disulfide-containing, 12.0A spacer arm and cleavable with reducing agents.

Thermo Scientific™ Pierce™ KMUH Crosslinker

Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (19.0A) crosslinker containing maleimide and hydrazide groups.

Thermo Scientific™ Pierce™ DMS Crosslinker

Crosslink amines to amines with dimethyl suberimidate (CAS 34490-86-3); composed of imidoester groups at either end of a membrane-permeable, 8-atom spacer arm.

Thermo Scientific™ Pierce™ BM(PEG)2 Crosslinker

Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 2-unit (14.7A) PEG spacer arm.

Thermo Scientific™ Pierce™ DTBP Crosslinker

Crosslink amines to amines with dithiobispropionimidate; composed of imidoester groups at either end of a reducible (cleavable) disulfide-containing spacer arm.

Thermo Scientific™ Pierce™ DTSSP Crosslinker

Crosslink amines to amines with Sulfo-DSP; composed of sulfo-NHS-ester groups and disulfide spacer arm (12.0A) that is cleavable with reducing agents.

Thermo Scientific Pierce™ EDC, No-Weigh™ Format Crosslinker

Thermo Scientific™ Pierce™ EDC is carboxyl- and amine-reactive zero-length crosslinker. EDC reacts with carboxyl group first and forms amine-reactive O-acylisourea intermediate that quickly reacts with amino group to form amide bond with release of isourea by-product.

Thermo Scientific™ EZ-Link™ Maleimide-PEG2-Biotin No-Weigh™ Format

Thermo Scientific™ EZ-Link™ Maleimide-PEG2-Biotin No-Weigh™ Format is a mid-length, maleimide-activated, sulfhydryl-reactive biotinylation reagent that contains a 2-unit ethylene glycol in its spacer arm for increased water-solubility characteristics.

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